FlyBase Allele Report: Dmel\mid[los1]

General Information

Symbol

Dmel\mid^los1

Species

D. melanogaster

Name

FlyBase ID

FBal0051368

Feature type

allele

Associated gene

Dmel\mid

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

diepoxybutane

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Gaziova and Bhat, 2009)

Mutagen

diepoxybutane

(Kolodziej et al., 1995)

Progenitor genotype

Cytology

Description

A 22 bp deletion resulting in a deletion of 7 amino acids at position 321 and a frame shift leading to a STOP codon at amino acid position 350. Thus the resulting protein is predicted to have 28 amino acids that are different from wild type, in addition to the truncation.

(Gaziova and Bhat, 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2L:5,465,933..5,465,954 [+]

Comment:

Reported as a 22 bp deletion resulting in deletion of 7 amino acids at position 321 and a frame shift leading to a stop codon after 28 novel amino acids.

(Gaziova and Bhat, 2009)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | embryonic stage

(Gaziova and Bhat, 2009)

abnormal neuroanatomy | embryonic stage (with Df(2L)Exel6012)

(Gaziova and Bhat, 2009)

abnormal neuroanatomy | embryonic stage (with mid¹)

(Gaziova and Bhat, 2009)

abnormal neuroanatomy | embryonic stage (with mid²)

(Gaziova and Bhat, 2009)

Phenotype Manifest In

chordotonal organ & axon | lateral

(Kolodziej et al., 1995)

embryonic/larval hypodermal muscle cell

(Kumar et al., 2015)

larval longitudinal connective

(Kolodziej et al., 1995)

larval RP2 motor neuron | ectopic

(Gaziova and Bhat, 2009)

larval RP2 motor neuron | ectopic (with Df(2L)Exel6012)

(Gaziova and Bhat, 2009)

larval RP2 motor neuron | ectopic (with mid¹)

(Gaziova and Bhat, 2009)

larval RP2 motor neuron | ectopic (with mid²)

(Gaziova and Bhat, 2009)

symmetrical commissure

(Kolodziej et al., 1995)

Detailed Description

Statement

Reference

mid^los1/mid^los1 embryos show loss of lateral transverse muscles.

(Kumar et al., 2015)

An additional, ectopic eve-postive neuron is observed within the nerve cord in 25-41% of hemisegments in ~14 hour-old mutant embryos. In mid^los1/mid² embryos, the ectopic neuron is seen in 24-25% of hemisegments; in mid^los1/mid¹ embryos, the ectopic neuron is seen in 30-39% of hemisegments; in mid^los1/Df(2L)Exel6012 embryos, the ectopic neuron is seen in 57-65% of hemisegments. Further experiments suggest this is an ectopic RP2 (eRP2) neuron, resulting from the transformation of a wild type neuron termed the 'M-neuron', with an ectopic GMC-1-like cell and a RP2 sib-like cell also present. This extra RP2/sib lineage is formed 2-2.5 hours after the development of the bona fide RP2 lineage.

(Gaziova and Bhat, 2009)

Mutant phenotype as assayed by Ecol\lacZ^rp staining: commissural subset and longitudinal connectives have gaps. Mutant phenotype of lateral chordotonal axons includes: shorter axons, defasciculated axons or dorsally routed axons.

(Kolodziej et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

mid^los1 has abnormal neuroanatomy | embryonic stage phenotype, suppressible by Df(2L)ED773

(Gaziova and Bhat, 2009)

mid^los1 has abnormal neuroanatomy | embryonic stage phenotype, suppressible by wg^l-17

(Gaziova and Bhat, 2009)

Other

Statement

Reference

insc²², mid^los1 has abnormal neuroanatomy | embryonic stage phenotype

(Gaziova and Bhat, 2009)

mid^los1, numb⁷⁹⁶ has abnormal neuroanatomy | embryonic stage phenotype

(Gaziova and Bhat, 2009)

Phenotype Manifest In

Suppressed by

Statement

Reference

mid^los1 has larval RP2 motor neuron | ectopic phenotype, suppressible by Df(2L)ED773

(Gaziova and Bhat, 2009)

mid^los1 has larval RP2 motor neuron | ectopic phenotype, suppressible by wg^l-17

(Gaziova and Bhat, 2009)

Other

Statement

Reference

insc²², mid^los1 has larval RP2 motor neuron | increased number phenotype

(Gaziova and Bhat, 2009)

Additional Comments

Genetic Interactions

Statement

Reference

In mid^los1, insc²² mutants, two ectopic RP2s are observed.

In mid^los1, numb⁷⁹⁶ mutants, two ectopic RP2 sib cells are observed with complete penetrance.

The ectopic RP2 neuron seen in mid^los1 mutants is not observed in any animals that are also mutant for Df(2L)ED773 (which deletes nub and pdm2), and is missing in 75-96% of the hemisegments in mid^los1, wg^l-17 double mutants.

(Gaziova and Bhat, 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

mid^los1 is partially rescued by mid^UAS.cGa/Scer\GAL4^wg.PM

(Gaziova and Bhat, 2009)

mid^los1 is partially rescued by mid^UAS.cGa/Scer\GAL4^sca.PU

(Gaziova and Bhat, 2009)

mid^los1 is partially rescued by mid^UAS.cGa/Scer\GAL4^ftz.ng

(Gaziova and Bhat, 2009)