FlyBase Allele Report: Dmel\tilB[1]

General Information

Symbol

Dmel\tilB¹

Species

D. melanogaster

Name

FlyBase ID

FBal0050603

Feature type

allele

Associated gene

Dmel\tilB

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

X ray

Nature of the Allele

Allele class

Mutagen

X ray

(Kernan et al., 1994)

Progenitor genotype

Cytology

Description

Nucleotide substitution: C?T.

(Kavlie et al., 2010)

Amino acid replacement: Q171term.

(Kavlie et al., 2010)

Amino acid replacement: ??term.

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:22,450,517..22,450,517 [+]

Nucleotide change:

C22450517T

Amino acid change:

Q171term | tilB-PA

Reported amino acid change:

Q171term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Kavlie et al., 2010, Kavlie et al., 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult antennal nerve

(Eberl et al., 2000)

spermatid axoneme

(Eberl et al., 2000)

Detailed Description

Statement

Reference

Homozygous males lack the sound-evoked potential in the antennal nerve in response to a pulse song stimulus, indicating deafness.

(Kavlie et al., 2010)

90% of mutant files show no or only weak entrainment to temperature cycles in assays studying locomotor rhythms.

(Sehadova et al., 2009)

tilB¹ homozygous larvae have reduced touch sensitivity compared to wild-type. Locomotion in these larvae is aberrant: compared to controls they are slower and they pause, turn and retreat more often. These defects in locomotion arise at least partially from defective peristaltic motion: stride period and % positive and negative flow are altered compared to wild-type, and the larvae spend the majority of a single peristaltic cycle in a retracted position. Despite their locomoter defects, these larvae exhibit normal phototaxic behaviour.

(Caldwell et al., 2003)

Mutant males demonstrate vigorous courtship including courtship songs. Relative amplitude of the sine song is higher than normal in mutant male songs.

(Tauber and Eberl, 2001)

Mutants show complete absence of sound-evoked courtship behavior. The sound evoked response (measured via the antennal nerve) is eliminated. No ultrastructural defect is evident in the Johnston's organ. In mutant alleles the spermatid axonemes are defective. Dynein arms are missing and the nexin link may be missing. Some axonemal profiles are split, as observed in elongation stage spermatids.

(Eberl et al., 2000)

Larval behavioural phenotype: homozygous larvae have very low sensitivity to touch. Homozygous larval motility is wild type. Less than 10% of adults are viable and the survivors are sedentary, are unable to walk upside down or climb the sides of culture vials. Nervous system morphology is indistinguishable from wild type. At a high transepithelial potential (TEP) the mechanoreceptor potential (MRP) is wild type.

(Kernan et al., 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

tilB²

(Eberl et al., 2000)

Rescued by

tilB¹ is rescued by tilB^+t3.5

(Kavlie et al., 2010)

Partially rescued by

tilB¹ is partially rescued by Scer\GAL4^tilB.PK/tilB^UAS.EGFP

(Kavlie et al., 2010)

tilB¹ is partially rescued by tilB^UAS.EGFP

(Kavlie et al., 2010)

tilB¹ is partially rescued by tilB^UAS.Tag:MYC/Scer\GAL4^tilB.PK

(Kavlie et al., 2010)

tilB¹ is partially rescued by tilB^UAS.Tag:MYC

(Kavlie et al., 2010)

tilB¹ is partially rescued by Scer\GAL4^tilB.PK/tilB^UASp.Venus

(Kavlie et al., 2010)

Comments

tilB^+t3.5 fully rescues the characteristic wild-type response to a pulse song stimulus in tilB¹ homozygous males.

Expression of either tilB^{Scer\UAS.T:Avic\GFP-EGFP} or tilB^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^tilB.PK rescues the deafness of tilB¹ males (assayed by measuring the sound-evoked response to a pulse song stimulus). tilB^{Scer\UAS.T:Avic\GFP-EGFP} and tilB^{Scer\UAS.T:Hsap\MYC} each also rescue the deafness of tilB¹ males in this assay in the absence of a Scer\GAL4 driver.

Expression of either tilB^{Scer\UAS.T:Avic\GFP-EGFP} or tilB^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^tilB.PK does not rescue the sterility of tilB¹ males.

Expression of tilB^{Scer\UAS.T:Avic\GFP-YFP.Venus} under the control of Scer\GAL4^tilB.PK partially rescues the deafness of tilB¹ males (assayed by measuring the sound-evoked response to a pulse song stimulus). No rescue is seen in the absence of a Scer\GAL4 driver.

(Kavlie et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

tilB¹

(Matsuo et al., 2014, Kavlie et al., 2010, Sehadova et al., 2009, Kavlie et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (10)

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