FlyBase Allele Report: Dmel\N[UAS.cBa]

General Information

Symbol

Dmel\N^UAS.cBa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Baker

FlyBase ID

FBal0048888

Feature type

allele

Associated gene

Dmel\N

Associated Insertion(s)

Carried in Construct

P{UAS-N.Ba}

Also Known As

UAS-Notch

Key Links

Transgenic product class

cDNA

(Baker and Schubiger, 1996)

wild_type

(Baker and Schubiger, 1996)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Baker and Schubiger, 1996)

wild_type

(Baker and Schubiger, 1996)

Mutagen

in vitro construct

(Baker and Schubiger, 1996)

Progenitor genotype

Carried in construct

P{UAS-N.Ba}

Cytology

Description

UASt regulatory sequences drive expression of a full length N cDNA fragment.

(Baker and Schubiger, 1996)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Babaoglan et al., 2009)

Phenotype Manifest In

dMP2 neuron

(Babaoglan et al., 2009)

vMP2 neuron

(Babaoglan et al., 2009)

Detailed Description

Statement

Reference

Both daughter cells adopt a vMP2 fate and extend their axons anteriorly in 14% of hemisegments when N^Scer\UAS.cBa is over expressed under the control of Scer\GAL4^pros.PMG. In all other cases, equal specification of dMP2 and vMP2 sibling cells is observed.

Expression of N^Scer\UAS.cBa under the control of Scer\GAL4^twi.PG has little effect on eve-positive pericardial cell or DA1 muscle cell number.

(Babaoglan et al., 2009)

Presence of Scer\GAL4^twi.PBa causes a normalised epidermal development. Can also normalise some but no all aspects of myogenesis in Df(1)N-54l9 mutants.

(Baker and Schubiger, 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^pros.PMG/N^UAS.cBa is a suppressor of abnormal neuroanatomy phenotype of spdo^G104

(Babaoglan et al., 2009)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

N^UAS.cBa, Scer\GAL4^twi.PG, spdo^G104 has embryonic pericardial cell phenotype, non-enhanceable by numb²

(Babaoglan et al., 2009)

Suppressor of

Statement

Reference

Scer\GAL4^pros.PMG/N^UAS.cBa is a suppressor of dMP2 neuron | ectopic phenotype of spdo^G104

(Babaoglan et al., 2009)

Scer\GAL4^pros.PMG/N^UAS.cBa is a suppressor of vMP2 neuron phenotype of spdo^G104

(Babaoglan et al., 2009)

N^UAS.cBa/Scer\GAL4^twi.PG is a suppressor of embryonic pericardial cell phenotype of spdo^G104

(Babaoglan et al., 2009)

N^UAS.cBa, Scer\GAL4^twi.PG, numb² is a suppressor of embryonic pericardial cell phenotype of spdo^G104

(Babaoglan et al., 2009)

N^UAS.cBa/Scer\GAL4^twi.PG is a suppressor of muscle cell of dorsal acute muscle 1 phenotype of spdo^G104

(Babaoglan et al., 2009)

N^UAS.cBa is a suppressor of larval intersegmental nerve phenotype of Abl^UAS.cFa, Scer\GAL4^unspecified

(Crowner et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of N^Scer\UAS.cBa under the control of Scer\GAL4^twi.PG in a spdo^G104 background increases eve-positive pericardial cell number and decreases DA1 muscle numbers compared to embryos mutant for spdo^G104.

Expression of N^Scer\UAS.cBa under the control of Scer\GAL4^pros.PMG in a spdo^G104 mutant background restores vMP2 development in the majority of hemisegments.

Expression of N^Scer\UAS.cBa under the control of Scer\GAL4^twi.PG in a numb² and spdo^G104 mutant background increases the number of eve-positive pericardial cells, and decreases the number of eve-positive DA1 muscle numbers compared to embryos mutant for spdo^G104. However, there is no change observed in this increased number of eve-positive pericardial cells between spdo^G104 embryos also expressing N^Scer\UAS.cBa under the control of Scer\GAL4^twi.PG alone, or in combination with numb².

N^Scer\UAS.cBa over expression by Scer\GAL4^twi.PG in a spdo^G104 mutant background increases the frequency at which both daughter cells adopt the svp-positive pericardial cell fate.

(Babaoglan et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

N^UAS.cBa/Scer\GAL4^elav.PLu rescues N^l1N-ts1

(Giniger, 1998)

Partially rescues

N^UAS.cBa/Scer\GAL4^elav.PLu partially rescues N^l1N-ts1

(Crowner et al., 2003)

Fails to rescue

N^UAS.cBa/Scer\GAL4^ato.3.6 fails to rescue N^l1N-ts1

(Hassan et al., 2000)

Comments

The addition of N^Scer\UAS.cBa driven by Scer\GAL4^elav.PLu largely rescues the ISNb bypass phenotype seen in N^l1N-ts1 animals. (13% bypass in rescued embryos versus 31% in N^l1N-ts1).

(Crowner et al., 2003)

The defects in axons entering the optic lobe seen in N^l1N-ts1 larvae lacking N⁺ activity are not rescued by N^Scer\UAS.cBa expressed under the control of Scer\GAL4^ato.3.6.

(Hassan et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

N^Scer\UAS.cBa

N^UAS.cBa

UAS-N

(Giniger, 1998)

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Baker

(Baker and Schubiger, 1996)

Secondary FlyBase IDs

References (6)

Report Sections

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