FlyBase Allele Report: Rat\CamKII-I[Ala.hs]

General Information

Symbol

Rat\CamKII-I^Ala.hs

Species

R. unknown

Name

FlyBase ID

FBal0048075

Feature type

allele

Associated gene

Rat\CamKII-I

Associated Insertion(s)

Carried in Construct

P{CamKII-I^Ala.hs}

Also Known As

ala2, UAS-ala, ala

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Griffith et al., 1993)

Progenitor genotype

Carried in construct

P{CamKII-I^Ala.hs}

Cytology

Description

Expression of a synthetic peptide, carrying an Ala in place of the Thr that is autophosphorylated, is driven by an Hsp70 promoter.

(Griffith et al., 1993)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

Rat\CamKII-I

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior

(Griffith et al., 1994)

abnormal neuroanatomy

(Beumer et al., 2002)

abnormal neuroanatomy, with Scer\GAL4^Toll-6-D42

(Chun-Jen Lin et al., 2011)

abnormal neurophysiology

(Yao and Wu, 2001, Griffith et al., 1994, Wang et al., 1994)

Phenotype Manifest In

bouton

(Beumer et al., 2002)

larval abdominal segmental nerve

(Wang et al., 1994)

larval VL3/4 motor neuron

(Wang et al., 1994)

larval VO1 motor neuron

(Wang et al., 1994)

larval VO2 motor neuron

(Wang et al., 1994)

neuromuscular junction

(Beumer et al., 2002)

neuron

(Yao and Wu, 2001)

synapse

(Griffith et al., 1994)

synapse, with Scer\GAL4^Toll-6-D42

(Chun-Jen Lin et al., 2011)

Detailed Description

Statement

Reference

Expression of Rat\CamKII-I^Ala.hs in motor neurons under the control of Scer\GAL4^D42 decreases synapse number about two-fold.

(Chun-Jen Lin et al., 2011)

Expression of Rat\CamKII-I^Ala.hs (either alone or under the control of Scer\GAL4^hs.2sev) has little or no effect on free-running circadian period.

(Harrisingh et al., 2007)

Animals carrying Rat\CamKII-I^Ala.hs show an approximately 40% increase in bouton number at the neuromuscular junction.

(Beumer et al., 2002)

Neurons expressing Rat\CamKII-I^Ala.hs typically display prolonged action potential waveforms and abnormal spontaneous activity. The spike duration (the width of the at the inflection point during an action potential take-off in a spike train) is substantially prolonged in Rat\CamKII-I^Ala.hs neurons. During sustained step current injection, all-or-none spikes in the tonic and adaptive categories in Rat\CamKII-I^Ala.hs neurons exhibit a progressive decrease in spike size (along with an increase in spike duration

over the period of stimulation), in contrast to the well-maintained spike shape in wild-type cells. A subpopulation of Rat\CamKII-I^Ala.hs neurons fire highly irregular spike pattern in response to current injection. Long-lasting spontaneous bursting activity with sporadic occurrence and varying frequency is observed in 25-30% of Rat\CamKII-I^Ala.hs neurons compared with only 5% in wild-type cells, although the resting membrane potentials within spontaneous spike trains are also prolonged.

When Rat\CamKII-I^Ala.hs neurons are simulated by step current injections, the firing pattern, in particular the number of spikes in each spike train, varied to a much greater extent than that in wild-type cells.

(Yao and Wu, 2001)

Males do not show a block in memory, even in the apparent absence of learning, in courtship behaviour assays.

(Joiner and Griffith, 1997)

Homozygotes fail to court females. Physiological studies reveal supernumerary synaptic discharges.

(Griffith et al., 1994)

Individuals exhibit clear free-running locomotor activity rhythms.

(Levine et al., 1994)

Expression of P{CamKII-I^Ala.hs} with or without heat shock causes an impaired associative conditioning behavioural paradigm. Altered short term plasticity in synaptic transmission along with abnormal nerve terminal sprouting and directionality of outgrowth. Excitatory junctional currents (EJCs) are greater than normal.

(Wang et al., 1994)

Transformed flies fail to learn normally in two behavioural plasticity paradigms: acoustic priming (a nonassociative measure of sensitization) and courtship conditioning (a measure of associative learning).

(Griffith et al., 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Rat\CamKII-I^Ala.hs has abnormal circadian rhythm phenotype, enhanceable by Mmmm\PV^UAS.Tag:MYC/Scer\GAL4^P2.4.Pdf

(Harrisingh et al., 2007)

Suppressed by

Statement

Reference

Rat\CamKII-I^Ala.hs, Scer\GAL4^Toll-6-D42 has abnormal neuroanatomy phenotype, suppressible by Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^Toll-6-D42

(Chun-Jen Lin et al., 2011)

Other

Statement

Reference

Mmmm\PV^UAS.Tag:MYC, Rat\CamKII-I^Ala.hs, Scer\GAL4^P2.4.Pdf has abnormal circadian rhythm phenotype

(Harrisingh et al., 2007)

Phenotype Manifest In

Suppressed by

Statement

Reference

Rat\CamKII-I^Ala.hs, Scer\GAL4^Toll-6-D42 has synapse phenotype, suppressible by Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^Toll-6-D42

(Chun-Jen Lin et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC,T:Hsap\CAAX} in motor neurons with Rat\CamKII-I^Ala.hs, both under the control of Scer\GAL4^D42, suppresses the Rat\CamKII-I^Ala.hs-dependent decrease in synapse number. Synapse number is significantly increased in these double mutants and is indistinguishable from synapse number in larvae expressing Pi3K92E^{Scer\UAS.T:Hsap\MYC,T:Hsap\CAAX} alone.

(Chun-Jen Lin et al., 2011)

Flies expressing Rat\CamKII-I^Ala.hs under the control of Scer\GAL4^Oamb.4.4 in a Oamb^unspecified heterozygous background show significantly reduced ovulation levels compared to controls. Reduced ovulation levels are also seen when Scer\GAL80^ts.αTub84B is used to limit expression to 3 days prior to mating.

(Lee et al., 2009)

Flies expressing both Mmmm\PV^{Scer\UAS.T:Hsap\MYC} and Rat\CamKII-I^Ala.hs under the control of Scer\GAL4^P2.4.Pdf exhibit period lengthening that is significantly different from controls.

(Harrisingh et al., 2007)

Animals carrying Rat\CamKII-I^Ala.hs which are also mutant for mys^b9 show an approximately 20% increase in bouton number at the neuromuscular junction. Animals carrying Rat\CamKII-I^Ala.hs which are also mutant for mys^ts1 show an approximately 16% increase in bouton number at the neuromuscular junction.

(Beumer et al., 2002)

eag¹/Rat\CamKII-I^Ala.hs individuals also fail to court.

(Griffith et al., 1994)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Can be expressed without heat shock.

(Wang et al., 1994)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CamKII-I^Ala.hs

Rat\CamKII-I^Ala.hs

Name Synonyms

Secondary FlyBase IDs

References (15)

Report Sections

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