FlyBase Allele Report: Ecol\lacZ[Mef2.4.0]

General Information

Symbol

Ecol\lacZ^Mef2.4.0

Species

E. coli

Name

FlyBase ID

FBal0047442

Feature type

allele

Associated gene

Ecol\lacZ

Associated Insertion(s)

Carried in Construct

P{Mef2-lacZ-I}

Also Known As

dMEF2-lacZ

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Cripps et al., 1998, Schulz et al., 1996, Lilly et al., 1995)

Progenitor genotype

Carried in construct

P{Mef2-lacZ-I}

Cytology

Description

A 4kb EcoRI fragment containing Mef2 sequences from -3564bp to +521bp drives lacZ expression.

(Cripps et al., 1998)

lacZ expression is driven by a 4.0kb EcoRI fragment which includes 3.4kb of the Mef2 5' flanking region and 0.6kb of (non-protein coding) exon 1.

(Lilly et al., 1995)

Allele components

Component

Use(s)

Regulatory region(s)

Mef2_4kb-EcoRI/EcoRI

Encoded product / tool

lacZ

reporter enzyme

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 6 -- 10

mesoderm

(Lilly et al., 1995)

embryonic stage 11 -- 17

visceral mesoderm

(Lilly et al., 1995)

somatic mesoderm

(Lilly et al., 1995)

embryonic stage 14

somatic muscle primordium

(Lilly et al., 1995)

visceral muscle primordium

(Lilly et al., 1995)

Additional Information

Statement

Reference

Reporter gene expression is first observed in the ventral mesoderm at the late cellular blastoderm stage. During germband extension, it is expressed at high levels in all of the precursors of the visceral and somatic musculature and later throughout the differentiated muscles. No expression is observed in the dorsal vessel.

(Lilly et al., 1995)

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Ecol\lacZ^Mef2.4.0

lacZ^Mef2.4.0

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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