Only 34% of embryos derived from hemizygous btz1 females have abdominal defects.
Almost all embryos laid by btz1 mothers at 18oC lack pole cells but have a normal number of denticle belts. Most embryos develop into viable and fertile adults if the others are kept at 25oC. About a third of adult female progeny have only one gametic ovary. In btz1 Mutant ovaries the arrangement of microtubules appears normal.
Homozygous females yield progeny whose embryonic gonads are largely depeleted of germ cells. Phenotype is weaker at 25oC than 18oC, at 25oC embryos contain only 2.2 germ cells per gonad compared to 12.2 in wild type. As development progresses the number of germ cells increases (proliferation in late embryonic and early first instar stages) so that in adults gonads are either empty or apparently normal, all ovarioles are full of germ cells.
Mutant embryos fail to form pole cells and show weak abdominal cuticle defects.
btz1, osk2 has abdominal segment phenotype, enhanceable by CG748319
btz1 has primordial germ cell phenotype, enhanceable by CG748319
btz1, osk2 has abdominal segment phenotype
btz1, osk2/osk[+] has abdominal segment phenotype
btz[+]/btz1, osk2 has abdominal segment phenotype
The osk and stau localisation phenotypes seen in btz1 oocytes is enhanced in combination with eIF4AIII19. The loss of pole cells and the associated loss of ovaries is also enhanced: penetrance of this phenotype increases from 6% to 31%, for loss of at least one ovary. Embryos produced by btz1 females with one copy of osk2 show a partial loss of abdominal segments. This phenotype is enhanced by the presence of eIF4AIII19.
