FlyBase Allele Report: Dmel\sn[28]

General Information

Symbol

Dmel\sn²⁸

Species

D. melanogaster

Name

FlyBase ID

FBal0046496

Feature type

allele

Associated gene

Dmel\sn

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - genetic evidence

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Cant and Cooley, 1996, Cooley et al., 1996)

loss of function allele

(Cant et al., 1998)

Mutagen

ethyl methanesulfonate

(Cant and Cooley, 1996)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell migration

(Zanet et al., 2012)

abnormal cell polarity | embryonic stage 15

(Zanet et al., 2009)

female sterile

(Zanet et al., 2012, Zanet et al., 2009)

sterile | recessive

(Cant and Cooley, 1996)

Phenotype Manifest In

Detailed Description

Statement

Reference

In sn²⁸/sn²⁸ nurse cells, the nucleolus has a dispersed phenotype and fewer interconnected tubules than controls. The severity of the phenotype is lower at oogenesis stages 8, 9 and 10A compared to 10B, and in anterior nurse cells.

(Groen et al., 2015)

Macrophage migration is reduced in sn²⁸ mutant stage 15 embryos, with fewer macrophages dispersing from the head to the posterior 2/3 of the embryo along the ventral surface. The macrophages have fewer actin-rich filopodia and microspikes within lamellae.

Nurse cell actin bundle formation is disrupted in sn²⁸ mutants and females flies are sterile.

(Zanet et al., 2012)

Stage 14 sn²⁸ embryos show a substantial delay in the migration of plasmatocytes along the ventral midline. 28% of these embryos show "strong" migration defects. These defects persist at later stages - plasmatocytes remain aggregated in the ventral part of the embryo and are unable to reach the posterior region.

The number of plasmatocytes recruited to a laser-induced wound is significantly reduced in sn²⁸ mutants compared to controls. The migratory speed of sn²⁸ plasmatocytes is also reduced.

Plasmatocytes in stage 15 sn²⁸ embryos do not form polarized lamellar extensions, and instead exhibit an abnormal, flacid lamella that surrounds the whole cell. Cell polarity and lamellipodia dynamics are drastically reduced.

Nurse cell nuclei of stage 10 egg chambers are not sustained by actin bundles in sn²⁸ mutants.

(Zanet et al., 2009)

The phenotype is suppressed by qua^Germ.WT; sn²⁸ females carrying three copies of qua^Germ.WT are semi-fertile and produce about 50% the number of eggs produced by wild-type females. Cytoplasmic actin bundles are sparse and disorganised and mature eggs are 60-70% the size of wild-type eggs. sn²⁸ females carrying four copies of qua^Germ.WT are fully fertile. The egg phenotype is not rescued by qua^Germ.HL.

(Cant et al., 1998)

Bristles severely gnarled.

(Cant and Cooley, 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

qua^Germ.WT, sn²⁸ has female fertile phenotype

(Cant et al., 1998)

Phenotype Manifest In

Suppressed by

Statement

Reference

sn²⁸ has egg phenotype, suppressible by qua^Germ.WT

(Cant et al., 1998)

sn²⁸ has egg chamber phenotype, suppressible by qua^Germ.WT

(Cant et al., 1998)

NOT suppressed by

Statement

Reference

sn²⁸ has phenotype, non-suppressible by qua^Germ.HL

(Cant et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

The phenotype is suppressed by qua^Germ.WT; sn^S289N females carrying three copies of qua^Germ.WT are semi-fertile and produce about 50% the number of eggs produced by wild-type females. Cytoplasmic actin bundles are sparse and disorganised and mature eggs are 60-70% the size of wild-type eggs. sn^S289N females carrying four copies of qua^Germ.WT are fully fertile. The egg phenotype is not rescued by qua^Germ.HL.

(Cant et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

sn²⁸ is rescued by sn^UASp.EGFP/Scer\GAL4^VP16.osk

(Groen et al., 2015)

sn²⁸ is rescued by sn^UASp.EGFP/Scer\GAL4^{VP16.nanos.UTR}

(Zanet et al., 2012)

sn²⁸ is rescued by sn^UASp.EGFP/Scer\GAL4^sn.PZ

(Zanet et al., 2012)

sn²⁸ is rescued by Scer\GAL4^sn.PZ/sn^{S289A.UASp.GFP}

(Zanet et al., 2012)

sn²⁸ is rescued by sn^UASp.EGFP/Scer\GAL4^{VP16.nanos.UTR}

(Zanet et al., 2009)

sn²⁸ is rescued by Scer\GAL4^{VP16.nanos.UTR}/sn^{S52A.UASp.EGFP}

(Zanet et al., 2009)

sn²⁸ is rescued by Scer\GAL4^{VP16.nanos.UTR}/sn^{S52E.UASp.EGFP}

(Zanet et al., 2009)

Partially rescued by

sn²⁸ is partially rescued by Scer\GAL4^{VP16.nanos.UTR}/sn^{S289A.UASp.GFP}

(Zanet et al., 2012)

sn²⁸ is partially rescued by Scer\GAL4^sn.PZ/sn^{S289D.UASp.GFP}

(Zanet et al., 2012)

Not rescued by

sn²⁸ is not rescued by Scer\GAL4^{VP16.nanos.UTR}/sn^{S289D.UASp.GFP}

(Zanet et al., 2012)

Comments

Expressing sn^UASp.EGFP under the control of Scer\GAL4^VP16.osk rescues the disperse nucleolar phenotype in sn²⁸/sn²⁸ nurse cells.

(Groen et al., 2015)

Expression of sn^{Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^sn.PZ rescues the macrophage migration defects seen in sn²⁸ mutant stage 15 embryos. The reduction in the number of actin rich filopodia and microspikes within the lamellae of sn²⁸ mutant macrophages is also rescued.

Expression of sn^{Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} rescues the female sterility and actin bundle formation defects seen in sn²⁸ mutant nurse cells.

Expression of sn^{Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^sn.PZ rescues the macrophage migration defects seen in Df(1)C128 mutant stage 15 embryos.

Expression of sn^{S289A.Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^sn.PZ rescues the macrophage migration defects seen in sn²⁸ mutant stage 15 embryos. The reduction in the number of actin-rich filopodia and microspikes within the lamellae of sn²⁸ mutant macrophages is also rescued.

Expression of sn^{S289A.Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} rescues the actin bundle formation defects seen in sn²⁸ mutant nurse cells. Female sterility is restored in 80% of sn²⁸ mutant flies.

Expression of sn^{S289A.Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^sn.PZ rescues the macrophage migration defects seen in Df(1)C128 mutant stage 15 embryos.

Expression of sn^{S289D.Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^sn.PZ partially rescues the macrophage migration defects seen in sn²⁸ mutant stage 15 embryos.

Expression of sn^{S289D.Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} fails to rescue the female sterility and actin bundle formation defects seen in sn²⁸ mutant nurse cells.

Expression of sn^{S289D.Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^sn.PZ partially rescues the macrophage migration defects seen in Df(1)C128 mutant stage 15 embryos. The reduction in the number of actin-rich filopodia seen in sn²⁸ mutant macrophages is not rescued.

(Zanet et al., 2012)

Scer\GAL4^{nos.UTR.T:Hsim\VP16}-driven expression of sn^{Scer\UAS.P\T.T:Avic\GFP-EGFP} fully restores the formation of actin bundles around nurse cell nuclei that is normally lacking in sn²⁸ mutants. Female ferility is also restored.

Scer\GAL4^{nos.UTR.T:Hsim\VP16}-driven expression of sn^{S52A.Scer\UAS.P\T.T:Avic\GFP-EGFP} fully restores the formation of actin bundles around nurse cell nuclei that is normally lacking in sn²⁸ mutants. Female ferility is also restored.

Scer\GAL4^{nos.UTR.T:Hsim\VP16}-driven expression of sn^{S52E.Scer\UAS.P\T.T:Avic\GFP-EGFP} fully restores the formation of actin bundles around nurse cell nuclei that is normally lacking in sn²⁸ mutants. Female ferility is also restored

(Zanet et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

fascin^sn28

(Groen et al., 2015)

sn²⁸

(Dobramysl et al., 2021, Groen et al., 2015, Zanet et al., 2012, Zanet et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (8)

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