FlyBase Allele Report: Dmel\mei-41[RT1]

General Information

Symbol

Dmel\mei-41^RT1

Species

D. melanogaster

Name

FlyBase ID

FBal0046106

Feature type

allele

Associated gene

Dmel\mei-41

Associated Insertion(s)

P{}mei-41^RT1

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

PM hybrid dysgenesis

(Yamamoto et al., 1990)

P-element activity

(Banga et al., 1995, Hari et al., 1995, Banga et al., 1991)

Progenitor genotype

Associated Insertion(s)

P{}mei-41^RT1

Cytology

Description

Insertion of P-element into the same SalI-EcoRI genomic fragment that includes the insertion in mei-41^RT2, though in opposite orientation to P-element in mei-41^RT2.

(Banga et al., 1995)

Insertion of a P-element.

(Banga et al., 1991)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

X:16,393,366..16,393,366

(Kondo and Perrimon, 2011, Smolik and Jones, 2007, Banga et al., 1995, Hari et al., 1995, Banga et al., 1991, Yamamoto et al., 1990)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygous eye discs show severe defects in hydroxyurea-induced G2 arrest, with the number of mitotic cells remaining high after treatment, in contrast to wild type.

(Lee et al., 2012)

X-ray irradiation of wild-type third-instar wing imaginal discs induces cell cycle arrest within 1 hour. In contrast, X-ray irradiation of mei-41^RT1 mutant larvae fails to induce cell cycle arrest.

(Kondo and Perrimon, 2011)

Mutant larvae fail to undergo G2 arrest in response to ionizing radiation, in contrast to wild type (assayed by scoring mitotic cells in larval eye-antennal imaginal discs).

Mutant larvae fail to undergo intra-S phase arrest in response to ionizing radiation, in contrast to wild type (assayed by scoring mitotic cells in larval brains).

Mutants are hypersensitive to irradiation, showing only 3.3% eclosion after exposure to 10 Gray (90.2% of wild-type larvae eclose at this dose).

(Rickmyre et al., 2007)

Mutant larvae lack the normal checkpoint response (a steep decline in the number of G₂ cells entering mitosis) when irradiated with either 500 or 4000 rad of X rays.

(Bi et al., 2005)

Mutant wing discs do not show cell cycle block after X-ray irradiation (in contrast to wild type), with the number of mitotic cells in mutant irradiated wing discs being similar to the number of mitotic cells in untreated mutant wing discs.

(Xu et al., 2001)

The number of mitotic cells in the mei-41^RT1 eye disc is unchanged following irradiation, in contrast to wild-type discs where very few cells are in M phase after irradiation. The morphology of the eye disc and adult eye is normal in unirradiated mei-41^RT1 animals.

(Brodsky et al., 2000)

Homozygotes are sensitive to MMS and show female sterility.

(Banga et al., 1995)

Methyl methanesulfonate sensitive, exposure causes lethality. Frequency of XO males and XXY females suggests high rates of chromosome loss.

(Yamamoto et al., 1990)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

mei-41^D5/mei-41^RT1 has lethal | embryonic stage phenotype, enhanceable by MCPH1^Z1861/MCPH1[+]

(Rickmyre et al., 2007)

Suppressed by

Statement

Reference

mei-41^RT1, nej[+]/nej³ has abnormal mitotic cell cycle | dominant phenotype, suppressible by nej^+t16

(Smolik and Jones, 2007)

Enhancer of

Statement

Reference

mei-41^RT1 is an enhancer of abnormal mitotic cell cycle | third instar larval stage phenotype of tefu^unspecified

(Bi et al., 2005)

NOT Enhancer of

Statement

Reference

mei-41^RT1 is a non-enhancer of visible phenotype of Fcp1^c.UAS, Scer\GAL4^en-e16E

(Schauer et al., 2009)

mei-41^RT1 is a non-enhancer of lethal phenotype of Fcp1^RNAi.UAS.WIZ, Scer\GAL4^en-e16E

(Schauer et al., 2009)

Suppressor of

Statement

Reference

mei-41^RT1 is a suppressor of lethal - all die during pupal stage phenotype of Df(3R)d189/Df(3R)d58, moi^um.t4.6

(Komonyi et al., 2009)

NOT Suppressor of

Statement

Reference

mei-41^RT1 is a non-suppressor of visible phenotype of Fcp1^c.UAS, Scer\GAL4^en-e16E

(Schauer et al., 2009)

mei-41^RT1 is a non-suppressor of lethal phenotype of Fcp1^c.UAS, Scer\GAL4^da.G32

(Schauer et al., 2009)

mei-41^RT1 is a non-suppressor of lethal phenotype of Fcp1^RNAi.UAS.WIZ, Scer\GAL4^da.G32

(Schauer et al., 2009)

mei-41^RT1 is a non-suppressor of lethal phenotype of Fcp1^RNAi.UAS.WIZ, Scer\GAL4^en-e16E

(Schauer et al., 2009)

Other

Statement

Reference

mei-41^RT1, nej[+]/nej³ has abnormal mitotic cell cycle | dominant phenotype

(Smolik and Jones, 2007)

mei-41^RT1, tefu^unspecified has lethal | wandering third instar larval stage phenotype

(Bi et al., 2005)

mei-41^RT1, tefu^unspecified has abnormal developmental rate | wandering third instar larval stage phenotype

(Bi et al., 2005)

mei-41^RT1, tefu^unspecified has decreased occurrence of cell division | third instar larval stage phenotype

(Bi et al., 2005)

Phenotype Manifest In

Enhancer of

Statement

Reference

mei-41^RT1 is an enhancer of chromosome & neuroblast | third instar larval stage 2 phenotype of tefu^unspecified

(Bi et al., 2005)

mei-41^RT1 is an enhancer of chromosome & neuroblast | third instar larval stage 2 phenotype of nbs¹

(Bi et al., 2005)

NOT Enhancer of

Statement

Reference

mei-41^RT1 is a non-enhancer of wing phenotype of Fcp1^c.UAS, Scer\GAL4^en-e16E

(Schauer et al., 2009)

Suppressor of

Statement

Reference

Df(1)BSC772/mei-41^RT1 is a suppressor | maternal effect of dorsal appendage | maternal effect phenotype of CycG^HR7

(Nagel et al., 2012)

mei-41^RT1 is a suppressor of FlyBase_internalproperty type:chromosome:chromosome phenotype of Df(3R)d189/Df(3R)d58, moi^um.t4.6

(Komonyi et al., 2009)

mei-41^RT1 is a suppressor of FlyBase_internalproperty type:chromosome:chromosome phenotype of Df(3R)d189/moi^S096713, moi^um.t4.6

(Komonyi et al., 2009)

mei-41^D3/mei-41^RT1 is a suppressor of dorsal appendage phenotype of mus301⁰⁹⁴

(Masrouha et al., 2003)

mei-41^D3/mei-41^RT1 is a suppressor of dorsal appendage phenotype of spn-B^unspecified

(Masrouha et al., 2003)

mei-41^D3/mei-41^RT1 is a suppressor of egg chorion | maternal effect phenotype of spn-B^BU

(Ghabrial and Schupbach, 1999)

mei-41[+]/mei-41^RT1 is a suppressor of egg chorion | maternal effect phenotype of spn-B^BU

(Ghabrial and Schupbach, 1999)

NOT Suppressor of

Statement

Reference

mei-41^RT1 is a non-suppressor of wing phenotype of Fcp1^c.UAS, Scer\GAL4^en-e16E

(Schauer et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

The ventralised eggshell phenotype seen in eggs derived from homozygous CycG^HR7 females is suppressed in more than 90% of eggs if the females are also carrying mei-41^RT1/Df(1)BSC772.

(Nagel et al., 2012)

Df(3R)d58/Df(3R)d189, P{CaSpeR-DTLu^m} (i.e. moi mutants) die at later phases or even hatch when they are also mutant for mei-41^RT1.

P{lacW}moi^S096713/Df(3R)d189, P{CaSpeR-DTLu^m} (i.e. moi mutant) neuroblasts show fewer anaphase chromosome bridges when mei-41^RT1 is present.

Df(3R)d58/Df(3R)d189, P{CaSpeR-DTLu^m} (i.e. moi mutant) neuroblasts show fewer anaphase chromosome bridges when mei-41^RT1 is present.

(Komonyi et al., 2009)

MCPH1^Z1861 dominantly enhances the embryonic lethality caused by mei-41^RT1/mei-41^D5.

(Rickmyre et al., 2007)

The number of mitotic cells is slightly but significantly higher in hydroxyurea-treated discs from mei-41^RT1/nej³ transheterozygotes compared with heterozygous mei-41^RT1 controls.

Expression of nej^+t16 suppresses the mitosis phenotypes in response to hydroxyurea seen in mei-41^RT1/nej³ transheterozygotes.

(Smolik and Jones, 2007)

mei-41^RT1 ; tefu^stg double mutant larvae show complete loss of the checkpoint response when irradiated with either 500 or 4000 rad of X rays.

(Bi et al., 2005)

The chromosome fusion phenotype seen in third instar larval neuroblasts of tefu^unspecified mutants is enhanced from an average of approximately 3 fusions per nucleus to an average of 7 fusions per nucleus.

The nuclei of neuroblasts in nbs¹; mei-41^RT1 double mutant third instar larvae have 2.5 times as many as nbs¹ single mutants. Over 12% of the double mutant nuclei are polyploid (n = 106).

(Bi et al., 2005)

mei-41^D3/mei-41^RT1 partially suppresses the dorsal appendage defects of mus301⁰⁹⁴ homozygotes; 70% of eggs derived from double mutant females have wild-type dorsal appendages, 21% have fused dorsal appendages and 9% have no dorsal appendages. mei-41^D3/mei-41^RT1 partially suppresses the dorsal appendage defects of eggs derived from spn-B^unspecified females.

(Masrouha et al., 2003)

The eggshell patterning defects of eggs derived from spn-B^BU/spn-B^BU females are suppressed if the females also carry mei-41^RT1/+ or mei-41^D3/mei-41^RT1.

(Ghabrial and Schupbach, 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

4169

107611

Notes on Origin

Discoverer

Comments

mei-41^RT1 and mei-41^RT2 reside within 400bp of each other. Transposase induced reversion demonstrates a P-element insertion is responsible for the mutant phenotype.

(Yamamoto et al., 1990)

The chromosome carrying mei-41^RT1 is relatively stable in the absence of P\T^Δ2-3 (which encodes a functional transposase), however in the presence of P\T^Δ2-3 the stability of the mei-41^RT1 chromosome is severely reduced. This instability is reversed if P\T^Sal is also present.

(Banga et al., 1991)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

mei-41^RT1

(Neitzel et al., 2018, Lee et al., 2012, Nagel et al., 2012, Kondo and Perrimon, 2011, Christensen et al., 2009.2.28, Komonyi et al., 2009, Schauer et al., 2009, Rickmyre et al., 2007, Bi et al., 2005, Bi et al., 2005)

mei-41^RT

(Smolik and Jones, 2007)

Name Synonyms

Secondary FlyBase IDs

References (25)

Report Sections

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