FlyBase Allele Report: Dmel\esg[UAS.cFa]

General Information

Symbol

Dmel\esg^UAS.cFa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Fuse

FlyBase ID

FBal0045610

Feature type

allele

Associated gene

Dmel\esg

Associated Insertion(s)

Carried in Construct

P{UAS-esg.F}

Also Known As

UAS-esg

Key Links

Transgenic product class

cDNA

(Fuse et al., 1994)

wild_type

(Fuse et al., 1994)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Fuse et al., 1994)

wild_type

(Fuse et al., 1994)

Mutagen

in vitro construct

(Fuse et al., 1994)

Progenitor genotype

Carried in construct

P{UAS-esg.F}

Cytology

Description

esg cDNA, from the initiator Met to the terminator codon plus about 500 bases of 3' untranslated sequences, is expressed under the control of UASt regulatory sequences.

(Fuse et al., 1994)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

esg

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of larval stage | heat sensitive, with Scer\GAL4^l(1)BP-1

(Fuse et al., 1994)

some die during larval stage | heat sensitive, with Scer\GAL4^l(1)BP-1

(Fuse et al., 1994)

visible, with Scer\GAL4^455.2

(Fuse et al., 1999)

visible, with Scer\GAL4^elav-C155

(Fuse et al., 1999)

visible | somatic clone, with Scer\GAL4^Act5C.PI

(Goto and Hayashi, 1999)

Phenotype Manifest In

adult tracheal air sac | third instar larval stage | heat sensitive, with Scer\GAL4^btl.PS, Scer\GAL80^ts.αTub84B

(Wang et al., 2010)

dorsal air sac primordium tip cell | third instar larval stage | heat sensitive, with Scer\GAL4^btl.PS, Scer\GAL80^ts.αTub84B

(Wang et al., 2010)

embryonic/larval salivary gland | embryonic stage, with Scer\GAL4^l(1)BP-1

(Fuse et al., 1994)

embryonic/larval tracheal system, with Scer\GAL4^C38, Scer\GAL4^tracheal

(Samakovlis et al., 1996)

eye, with Scer\GAL4^elav-C155

(Fuse et al., 1999)

interommatidial bristle, with Scer\GAL4^elav-C155

(Fuse et al., 1999)

leg & trichome | somatic clone, with Scer\GAL4^Act5C.PI

(Goto and Hayashi, 1999)

leg | somatic clone, with Scer\GAL4^Act5C.PI

(Goto and Hayashi, 1999)

leg sensillum | somatic clone, with Scer\GAL4^Act5C.PI

(Goto and Hayashi, 1999)

ommatidium, with Scer\GAL4^elav-C155

(Fuse et al., 1999)

scutellar bristle & tormogen cell, with Scer\GAL4^455.2

(Fuse et al., 1999)

scutellar bristle & trichogen cell, with Scer\GAL4^455.2

(Fuse et al., 1999)

tracheal system, with Scer\GAL4^C38

(Samakovlis et al., 1996)

tracheal system, with Scer\GAL4^tracheal

(Samakovlis et al., 1996)

Detailed Description

Statement

Reference

Expression of esg^Scer\UAS.cFa under the control of Scer\GAL4^Act.PU the eyFLP technique does not result in an altered targeting pattern of CO[[2]] neurons.

(Hartl et al., 2011)

Expression of esg^Scer\UAS.cFa under the control of Scer\GAL4^btl.PS (by inactivating Scer\GAL80^{ts.alphaTub84B} through heat-shock), results in a multi-tip air sac phenotype.

(Wang et al., 2010)

Expression of esg^Scer\UAS.cFa under the control of Scer\GAL4^elav-C155 results in a roughening of the eye and a reduction in the number of interommatidial bristles. Expression of esg^Scer\UAS.cFa under the control of Scer\GAL4^455.2 results in a complete loss of shaft and socket of the macrochaetae of the scutellum.

(Fuse et al., 1999)

Clones in the leg expressing esg^Scer\UAS.cFa under the control of Scer\GAL4^Act5C.PI invaginate to form a vesicle inside the leg cuticle in a cell-autonomous manner. These clones are often associated with malformation. Proximal to the clone, genetically wild-type bristles and epidermal hairs, which normally point distally, are often reversed. Clones expressing esg^Scer\UAS.cFa under the control of Scer\GAL4^Act5C.PI induced in the distal tarsal region of the leg disc during the second larval instar stage show compact morphology, are round in shape and have smooth borders. Clones expressing esg^Scer\UAS.cFa under the control of Scer\GAL4^Act5C.PI induced in the coxa and trochanter regions of the leg disc have ragged borders.

(Goto and Hayashi, 1999)

Scer\GAL4^C38 or Scer\GAL4^tracheal induced expression causes ectopic tracheal connections, linking up terminal branches that normally never join.

(Samakovlis et al., 1996)

At 25^oC most larvae die before second larval instar. Reduction in salivary gland and salivary gland nuclei size.

(Fuse et al., 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

esg^UAS.cFa/Scer\GAL4^{Delta-05151-G} is a suppressor of increased occurrence of cell division | adult stage phenotype of Scer\GAL4^{Delta-05151-G}, sc^UAS.cUa

(Li et al., 2017)

esg^UAS.cFa/Scer\GAL4^{Delta-05151-G} is a suppressor of increased cell number | adult stage phenotype of Scer\GAL4^{Delta-05151-G}, sc^UAS.cUa

(Li et al., 2017)

esg^UAS.cFa/Scer\GAL4^{Delta-05151-G} is a suppressor of increased cell number | adult stage phenotype of Scer\GAL4^{Delta-05151-G}, ase^UAS.cUa

(Li et al., 2017)

esg^UAS.cFa/Scer\GAL4^elav.PLu is a suppressor of bang sensitive phenotype of jus^iso7.8

(Hekmat-Scafe et al., 2005)

esg^UAS.cFa/Scer\GAL4^elav.PLu is a suppressor of bang sensitive phenotype of para^bss1

(Hekmat-Scafe et al., 2005)

esg^UAS.cFa/Scer\GAL4^elav.PLu is a suppressor of bang sensitive phenotype of eas²

(Hekmat-Scafe et al., 2005)

esg^UAS.cFa is a suppressor of visible phenotype of Scer\GAL4^455.2, ase^UAS.cBa

(Fuse et al., 1999)

Phenotype Manifest In

Suppressor of

Statement

Reference

esg^UAS.cFa/Scer\GAL4^{Delta-05151-G} is a suppressor of enteroendocrine cell | adult stage phenotype of Scer\GAL4^{Delta-05151-G}, sc^UAS.cUa

(Li et al., 2017)

esg^UAS.cFa/Scer\GAL4^{Delta-05151-G} is a suppressor of adult midgut | adult stage phenotype of Scer\GAL4^{Delta-05151-G}, sc^UAS.cUa

(Li et al., 2017)

esg^UAS.cFa/Scer\GAL4^{Delta-05151-G} is a suppressor of enteroendocrine cell | adult stage phenotype of Scer\GAL4^{Delta-05151-G}, ase^UAS.cUa

(Li et al., 2017)

esg^UAS.cFa is a suppressor of macrochaeta | ectopic phenotype of Scer\GAL4^455.2, ase^UAS.cBa

(Fuse et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of esg^Scer\UAS.cFa suppresses the increased enteroendocrine cell number and the dividing cell number in adult midguts expressing sc^Scer\UAS.cUa under the control of Scer\GAL4^Dl-05151-G.

Co-expression of esg^Scer\UAS.cFa suppresses the increased enteroendocrine cell number in adult midguts expressing ase^Scer\UAS.cUa under the control of Scer\GAL4^Dl-05151-G.

(Li et al., 2017)

The bang sensitivity of bss¹/+ flies is partially suppressed by the expression of esg^Scer\UAS.cFa under the control of Scer\GAL4^elav.PLu.

(Hekmat-Scafe et al., 2005)

Expression of ase^Scer\UAS.cBa under the control of Scer\GAL4^455.2 results in extra macrochaetae. This phenotype is strongly suppressed by co-expression of esg^Scer\UAS.cFa.

(Fuse et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

esg^Scer\UAS.cFa

esg^UAS.cFa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Fuse

(Fuse et al., 1994)

Secondary FlyBase IDs

References (14)

Report Sections

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