Midline glia do not completely ensheath the axon commissures, and there is an absence of membrane projections into the commissures in stage 15 embryos expressing en^Scer\UAS.cGa under the control of Scer\GAL4^sim.P3.7.

Expression of en^Scer\UAS.cGa under the control of Scer\GAL4^T155 results in transformation of anterior compartment to posterior compartment structures in the tergites.

When expression is driven by Scer\GAL4^twi.PG and Scer\GAL4^how-24B pericardial and muscle progenitors are missing and only a few mesodermal cells remain. Visceral mesodermal cells and fat body are unaffected.

When expression is driven by Scer\GAL4^Ubx.PdC in clones in the Posterior compartment of the developing adult abdominal tergite there is generally little effect, with some exceptions. The abnormal clones are elongated and abut the A/P border for many cell diameters from the posterior side. They make a6/p3 cuticle, with irregular hairs. These clones originate in the A compartment and merge later into the P compartment. When expression is driven by Scer\GAL4^Ubx.PdC in clones in the Anterior compartment of the developing adult abdominal tergite, clones are large and have reversed polarity (and induce reversed polarity) with an ectopic P region. New A/P and P/A borders are formed at the anterior and posterior limits of the clone. Marked bristles are rare, suggesting a posterior identity. In the most posterior parts of the A compartment larger clones are rare, and, when expression is driven by Scer\GAL4^69B, have p3 identity (with hairs). Hairs in front of the clone are oriented normally, behind the clone they are reversed. Clones induced in pupal stages are small and p1/p2 identity (in the anterior domain) or a6/p3, with varying polarities (in the posterior domain). The type of cuticle is characteristic of the segment in which it is found.

Scer\GAL4^twi.PGa-mediated overexpression of en leads to an expansion of the primary clusters resulting in a continuous dorsolateral fat body primordium in parasegments 4-9. Expression also causes expansion of the visceral mesoderm primordia and somatic gonad primordia. Primordia of the somatic muscles and heart are abolished. At stage 11 Scer\GAL4^zen.Kr.PF-mediated expression expands the dorsolateral fat body primordium in parasegments 6-8, the secondary central fat body clusters are missing in parasegments 6-8.

Scer\GAL4^arm.PS mediated expression causes small and spherical embryos, unsegmented with a central stripe of weak denticles of row 1 type.

When expression is driven by Scer\GAL4^Bx-MS1096, anterior dorsal wing develops a posterior pattern. Double row hairs and the axillary cord appear in the anterior compartment. When expression is driven by Scer\GAL4^71B or Scer\GAL4^C-734, vein pattern of part of the anterior compartment is transformed into posterior pattern. When expression is driven by Scer\GAL4^30A, anterior wing patterns are duplicated, the costa is transformed into axillary cord, along with a duplication of adjacent anterior patterns. Scer\GAL4^Bx-MS1096 can also generate anterior pattern in posterior positions, such that posterior compartments resemble those of en¹/en¹ or en¹/en².