FlyBase Allele Report: Scer\GAL4[Ubx.PdC]

General Information

Symbol

Scer\GAL4^Ubx.PdC

Species

S. cerevisiae

Name

Ultrabithorax promoter construct of de Celis

FlyBase ID

FBal0066072

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{GAL4-lacZ-Ubx(-FRT).dC}

Also Known As

Ubx-Gal4, UbxGal4

Transgenic product class

Mutagen

FLPase

Nature of the Allele

Transgenic product class

Mutagen

FLPase

(Callejo et al., 2006, Cook et al., 2004, Sakata, 2004, Torroja et al., 2004, Baena-Lopez and Garcia-Bellido, 2003, Azpiazu and Morata, 2002, Casal et al., 2002, Moreno et al., 2002, Moreno et al., 2002, Azpiazu and Morata, 2000, Baonza et al., 2000, de Celis and Bray, 2000, Phillips et al., 1999, Rauskolb and Irvine, 1999, de Celis and Bray, 1997)

Progenitor genotype

Scer\GAL4^FRT.Ubx

(Callejo et al., 2006, Cook et al., 2004, Sakata, 2004, Torroja et al., 2004, Baena-Lopez and Garcia-Bellido, 2003, Azpiazu and Morata, 2002, Casal et al., 2002, Moreno et al., 2002, Moreno et al., 2002, Tree et al., 2002, Azpiazu and Morata, 2000, Baonza et al., 2000, de Celis and Bray, 2000, Phillips et al., 1999, Rauskolb and Irvine, 1999, de Celis and Bray, 1997)

Carried in construct

P{GAL4-lacZ-Ubx(-FRT).dC}

Cytology

Description

The FRT cassette has been excised, allowing the Ubx promoter to drive expression of a coding cassette composed of GAL4 and lacZ (the two coding regions are separated by an internal ribosome entry site).

(de Celis and Bray, 1997)

Allele components

Component

Use(s)

Encoded product / tool

GAL4

binary expression system - driver

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

wing disc

(de Celis and Bray, 1997)

third instar larval stage

wing disc

(de Celis and Bray, 1997)

adult stage

adult abdomen

(Lawrence et al., 1999)

cuticle of adult abdomen

(Lawrence et al., 1999)

Additional Information

Statement

Reference

Designed for clonal analysis in wing disc and adult wing: ScerGAL4 expressed in cells that have lost the FRT.f[+] element; used for clonal analysis the gene of interest, when appropriate UAS construct present; clones are simultaneously marked by expression of EcollacZ in the imaginal disc and by f[-] phenotype in the adult wing. Stocks are attached to progenitor construct P{GAL4-lacZ-Ubx(FRT.f⁺).dC}; see ScerGAL4^ScerFRT.Ubx elsewhere in this table.

(FlyBase Curators, 2017)

Expression in abdomen inferred on basis of EcollacZ^Ubx.PdCRA expression.

(Lawrence et al., 1999)

Used for clonal analysis in wing disc; ScerGAL4 expressed in marked clones; used to drive gene of interest.

(de Celis and Bray, 1997)

Marker for

Reflects expression of

Ubx

(de Celis and Bray, 1997)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body color | somatic clone, with bi^UAS.cLa

(Lawrence et al., 2002)

abnormal cell polarity | cell non-autonomous, with pk^UAS.cGa

(Tree et al., 2002)

abnormal cell polarity | cell non-autonomous | somatic clone, with fj^UAS.cZa

(Casal et al., 2002)

abnormal planar polarity, with en^UAS.cGa

(Lawrence et al., 1999)

abnormal planar polarity | cell non-autonomous | somatic clone, with fz¹⁵, fz^UAS.cSa

(Lawrence et al., 2004)

abnormal planar polarity | cell non-autonomous | somatic clone, with fz^UAS.cSa

(Lawrence et al., 2004)

abnormal planar polarity | cell non-autonomous | somatic clone, with mirr^UAS.cMa

(Calleja et al., 2000)

abnormal planar polarity | cell non-autonomous | somatic clone, with pnr^UAS.cHa

(Calleja et al., 2000)

abnormal planar polarity | somatic clone, with fz¹⁵, fz^UAS.cSa

(Lawrence et al., 2004)

abnormal planar polarity | somatic clone, with fz^UAS.cSa

(Lawrence et al., 2004)

cell lethal, with brk^UAS.cJa

(Moreno et al., 2002)

increased cell number | somatic clone | third instar larval stage, with wg^UAS.cLb

(de Celis and Bray, 1997)

increased occurrence of cell division | somatic clone | third instar larval stage, with wg^UAS.cLb

(de Celis and Bray, 1997)

neoplasia | adult stage | somatic clone, with Delta^UAS.cJa

(Baonza and Garcia-Bellido, 2000)

visible, with Delta^UAS.cDa

(de Celis and Bray, 1997)

visible, with Delta^UAS.cJa

(Baonza and Garcia-Bellido, 2000)

visible, with dsh^UAS.cAa

(Phillips et al., 1999)

visible, with en^UAS.cGa

(Lawrence et al., 1999)

visible, with N^ECN.UAS

(de Celis and Bray, 1997)

visible, with N^int.G.UAS

(de Celis and Bray, 1997)

visible, with N^UAS.cBb

(de Celis and Bray, 1997)

visible, with Ser^UAS.cSa

(Baonza and Garcia-Bellido, 2000, de Celis and Bray, 1997)

visible, with wg^UAS.cLa

(Baonza and Garcia-Bellido, 2000)

visible, with wg^UAS.cLb

(de Celis and Bray, 1997)

visible | cell non-autonomous | somatic clone, with hth^UAS.cPa

(Azpiazu and Morata, 2002)

visible | cell non-autonomous | somatic clone, with N^int.G.UAS

(Baonza and Garcia-Bellido, 2000)

visible | somatic clone, with E(spl)m4-BFM^UAS.cAa

(Apidianakis et al., 1999)

visible | somatic clone, with hh^N.UAS.GFP

(Callejo et al., 2006)

visible | somatic clone, with hth^UAS.cPa

(Azpiazu and Morata, 2000)

visible | somatic clone, with tsh^UAS.cGCa

(Azpiazu and Morata, 2000)

visible | somatic clone, with vg^UAS.cZa

(Baena-Lopez and Garcia-Bellido, 2003)

Phenotype Manifest In

adult cuticle & abdomen | somatic clone, with bi^UAS.cLa

(Lawrence et al., 2002)

adult cuticle | cell non-autonomous | somatic clone, with fj^UAS.cZa

(Casal et al., 2002)

costal cell | somatic clone, with hh^N.UAS.GFP

(Callejo et al., 2006)

embryonic epidermis, with pnr^UAS.cHa

(Herranz and Morata, 2001)

femur | cell non-autonomous | somatic clone, with hth^UAS.cPa

(Azpiazu and Morata, 2002)

larval ventral nerve cord, with pnr^UAS.cHa

(Herranz and Morata, 2001)

leg | cell non-autonomous | somatic clone, with hth^UAS.cPa

(Azpiazu and Morata, 2002)

leg | somatic clone, with vg^UAS.cZa

(Baena-Lopez and Garcia-Bellido, 2003)

macrochaeta, with dsh^UAS.cAa

(Phillips et al., 1999)

macrochaeta & thorax | somatic clone, with Ser^{Y1248S.F2I.UAS}

(Li and Baker, 2004)

macrochaeta & thorax | supernumerary | somatic clone, with Ser^{Y1248S.F2I.UAS}

(Li and Baker, 2004)

macrochaeta & thorax | supernumerary | somatic clone | cell autonomous, with Delta::Ser^{Delta-E.Ser-I.UAS}

(Li and Baker, 2004)

macrochaeta | cell non-autonomous | somatic clone, with fj^UAS.cZa

(Casal et al., 2002)

macrochaeta | ectopic | somatic clone, with hh^N.UAS.GFP

(Callejo et al., 2006)

macrochaeta | ectopic | somatic clone, with pnr^UAS.cHa

(Calleja et al., 2000)

mesothoracic tergum | cell autonomous | ectopic | somatic clone, with pnr^UAS.cHa

(Calleja et al., 2000)

mesothoracic tergum | lateral | somatic clone, with pnr^UAS.cHa

(Calleja et al., 2000)

mesothoracic tergum | somatic clone, with hh^N.UAS.GFP

(Callejo et al., 2006)

mesothoracic tergum | somatic clone, with vg^UAS.cZa

(Baena-Lopez and Garcia-Bellido, 2003)

mesothoracic tergum | somatic clone, with wg^UAS.cLa

(Baonza and Garcia-Bellido, 2000)

metatarsus | cell non-autonomous | somatic clone, with hth^UAS.cPa

(Azpiazu and Morata, 2002)

microchaeta | cell non-autonomous | somatic clone, with fj^UAS.cZa

(Casal et al., 2002)

microchaeta | ectopic | somatic clone, with pnr^UAS.cHa

(Calleja et al., 2000)

microchaeta | somatic clone, with E(spl)m4-BFM^UAS.cAa

(Apidianakis et al., 1999)

postnotum | cell autonomous | ectopic | somatic clone, with pnr^UAS.cHa

(Calleja et al., 2000)

tarsal segment | cell non-autonomous | somatic clone, with hth^UAS.cPa

(Azpiazu and Morata, 2002)

tergite | cell autonomous | somatic clone, with mirr^UAS.cMa

(Calleja et al., 2000)

tergite | cell non-autonomous | somatic clone, with fj^UAS.cZa

(Casal et al., 2002)

tergite anterior compartment, with en^UAS.cGa

(Lawrence et al., 1999)

tergite posterior compartment, with en^UAS.cGa

(Lawrence et al., 1999)

tibia | cell non-autonomous | somatic clone, with hth^UAS.cPa

(Azpiazu and Morata, 2002)

trichome & pleural membrane | somatic clone, with fz¹⁵, fz^UAS.cSa

(Lawrence et al., 2004)

trichome & pleural membrane | somatic clone, with fz^UAS.cSa

(Lawrence et al., 2004)

trichome & pleural membrane | somatic clone | cell non-autonomous, with fz¹⁵, fz^UAS.cSa

(Lawrence et al., 2004)

trichome & pleural membrane | somatic clone | cell non-autonomous, with fz^UAS.cSa

(Lawrence et al., 2004)

ventral denticle belt, with pnr^UAS.cHa

(Herranz and Morata, 2001)

wing, with btl::Egfr^λ.UAS

(Wang et al., 2000)

wing, with N^ECN.UAS

(de Celis and Bray, 1997)

wing, with N^int.G.UAS

(de Celis and Bray, 1997)

wing, with N^UAS.cBb

(de Celis and Bray, 1997)

wing, with wg^UAS.cLb

(de Celis and Bray, 1997)

wing | cell non-autonomous | somatic clone, with N^int.G.UAS

(Baonza and Garcia-Bellido, 2000)

wing | dorsal, with Delta^UAS.cDa

(de Celis and Bray, 1997)

wing | ectopic | somatic clone, with wg^UAS.cLa

(Baonza and Garcia-Bellido, 2000)

wing | somatic clone, with hth^UAS.cPa

(Azpiazu and Morata, 2000)

wing | somatic clone, with pnr^UAS.cHa

(Calleja et al., 2000)

wing | somatic clone, with Ser^UAS.cSa

(Baonza and Garcia-Bellido, 2000)

wing | somatic clone, with tsh^UAS.cGCa

(Azpiazu and Morata, 2000)

wing | somatic clone, with vg^UAS.cZa

(Baena-Lopez and Garcia-Bellido, 2003)

wing | ventral, with Ser^UAS.cSa

(de Celis and Bray, 1997)

wing blade dorsal compartment | somatic clone, with Delta^UAS.cJa

(Baonza and Garcia-Bellido, 2000)

wing disc, with brk^UAS.cJa

(Moreno et al., 2002)

wing disc | somatic clone, with egr^UAS.cMa

(Moreno et al., 2002)

wing hair, with pk^UAS.cGa

(Tree et al., 2002)

wing hair | ectopic | somatic clone, with vg^UAS.cZa

(Baena-Lopez and Garcia-Bellido, 2003)

wing hinge | ectopic, with tsh^UAS.cGCa

(Azpiazu and Morata, 2000)

wing hinge | somatic clone, with wg^UAS.cLa

(Baonza and Garcia-Bellido, 2000)

wing hinge primordium | somatic clone | third instar larval stage, with wg^UAS.cLb

(de Celis and Bray, 1997)

wing margin | ectopic | somatic clone, with hth^UAS.cPa

(Azpiazu and Morata, 2000)

wing margin | ectopic | somatic clone, with N^int.G.UAS

(Baonza and Garcia-Bellido, 2000)

wing margin | ectopic | somatic clone, with wg^UAS.cLa

(Baonza and Garcia-Bellido, 2000)

wing margin | somatic clone, with hth^UAS.cPa

(Azpiazu and Morata, 2000)

wing sclerite | ectopic, with tsh^UAS.cGCa

(Azpiazu and Morata, 2000)

wing vein, with Delta^UAS.cDa

(de Celis and Bray, 1997)

wing vein, with N^ECN.UAS

(de Celis and Bray, 1997)

wing vein, with Ser^UAS.cSa

(de Celis and Bray, 1997)

wing vein | cell non-autonomous | ectopic | somatic clone, with ab^UAS.cBa

(Cook et al., 2004)

wing vein | ectopic, with Ser^UAS.cSa

(de Celis and Bray, 1997)

wing vein | ectopic | somatic clone, with hh^N.UAS.GFP

(Callejo et al., 2006)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^Ubx.PdC, egr^UAS.cMa has increased cell death phenotype, suppressible by Scer\GAL4^Ubx.PdC/puc^UAS.cMa

(Moreno et al., 2002)

Scer\GAL4^Ubx.PdC, egr^UAS.cMa has increased cell death phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^Ubx.PdC

(Moreno et al., 2002)

Scer\GAL4^Ubx.PdC, brk^UAS.cJa has cell lethal phenotype, suppressible by Scer\GAL4^Ubx.PdC/puc^UAS.cMa

(Moreno et al., 2002)

Scer\GAL4^Ubx.PdC, brk^UAS.cJa has cell lethal phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^Ubx.PdC

(Moreno et al., 2002)

Suppressor of

Statement

Reference

Scer\GAL4^Ubx.PdC/puc^UAS.cMa is a suppressor of increased cell death phenotype of Scer\GAL4^Ubx.PdC, egr^UAS.cMa

(Moreno et al., 2002)

BacA\p35^UAS.cHa, Scer\GAL4^Ubx.PdC is a suppressor of increased cell death phenotype of Scer\GAL4^Ubx.PdC, egr^UAS.cMa

(Moreno et al., 2002)

Scer\GAL4^Ubx.PdC/puc^UAS.cMa is a suppressor of cell lethal phenotype of Scer\GAL4^Ubx.PdC, brk^UAS.cJa

(Moreno et al., 2002)

BacA\p35^UAS.cHa, Scer\GAL4^Ubx.PdC is a suppressor of cell lethal phenotype of Scer\GAL4^Ubx.PdC, brk^UAS.cJa

(Moreno et al., 2002)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^Ubx.PdC, egr^UAS.cMa has wing disc phenotype, suppressible by Scer\GAL4^Ubx.PdC/puc^UAS.cMa

(Moreno et al., 2002)

Scer\GAL4^Ubx.PdC, egr^UAS.cMa has wing disc phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^Ubx.PdC

(Moreno et al., 2002)

Scer\GAL4^Ubx.PdC, brk^UAS.cJa has wing disc phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^Ubx.PdC

(Moreno et al., 2002)

Scer\GAL4^Ubx.PdC, brk^UAS.cJa has wing disc phenotype, suppressible by Scer\GAL4^Ubx.PdC/puc^UAS.cMa

(Moreno et al., 2002)

Suppressor of

Statement

Reference

Scer\GAL4^Ubx.PdC/puc^UAS.cMa is a suppressor of wing disc phenotype of Scer\GAL4^Ubx.PdC, egr^UAS.cMa

(Moreno et al., 2002)

BacA\p35^UAS.cHa, Scer\GAL4^Ubx.PdC is a suppressor of wing disc phenotype of Scer\GAL4^Ubx.PdC, egr^UAS.cMa

(Moreno et al., 2002)

BacA\p35^UAS.cHa, Scer\GAL4^Ubx.PdC is a suppressor of wing disc phenotype of Scer\GAL4^Ubx.PdC, brk^UAS.cJa

(Moreno et al., 2002)

Scer\GAL4^Ubx.PdC/puc^UAS.cMa is a suppressor of wing disc phenotype of Scer\GAL4^Ubx.PdC, brk^UAS.cJa

(Moreno et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Exists in clones, not reported as germ-line transmitted. Scer\GAL4 and Ecol\lacZ are expressed from a dicistronic transcript in this construct.

(de Celis and Bray, 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

GAL4^Ubx.PdC

Scer\GAL4^Ubx.PdC

Name Synonyms

Ultrabithorax promoter construct of de Celis

(de Celis and Bray, 1997)

Secondary FlyBase IDs

References (32)

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