Amino acid replacement: S99N. S99N falls in helix 3 of the DNA-binding domain.
G18133027A
S99N | Hsf-PA; S99N | Hsf-PB; S99N | Hsf-PC; S99N | Hsf-PD
Homozygotes arrest at the 1st or 2nd larval instar stage and die after 2-3 days without any further growth or development. Viability is rescued in homozygous or hemizygous Hsf2/Df(2R)PclP2 flies carrying Hsf+t8. Females mosaic for homozygous clones in the germ line are sterile.