Encodes a maternal hb mRNA in which the endogenous wild-type nos response elements (NREs) have been deleted, and two copies of a mutant NRE (each bearing the nucleotide substitutions T2G and T3G) have been inserted.
Construct: Tandem dinucleotide substitution within the NRE.
viable
Embryos expressing hbNRE5 have 7-8 abdominal segments, and most embryos develop into viable adults.
Embryos exhibit a normal abdominal segmentation pattern.