4.8kb EcoRI Adh fragment from position -1320 through to the 3' flanking sequences, all of the cis-acting DNA sequences necessary for the major tissue specificities of Adh expression.
Adh expression has correct tissue specificity but Adh protein level of adult flies is reduced by two fold and levels in third instar larval by 10-fold.
Carried in a plasmid, expressed in E.coli cells and injected into preblastoderm embryos. Larvae and adults developed from these embryos are dissected and stained for Adh activity.
Adh expression has correct tissue specificity but Adh protein level of adult flies is reduced by two fold and levels in third instar larval by 10-fold.
Carried in a plasmid, expressed in E.coli cells and injected into preblastoderm embryos. Larvae and adults developed from these embryos are dissected and stained for Adh activity.