FB2024_03 , released June 25, 2024
Allele: Ecol\lacZUbx.35UZ
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General Information
Symbol
Ecol\lacZUbx.35UZ
Species
E. coli
Name
FlyBase ID
FBal0042137
Feature type
allele
Associated gene
Ecol\lacZ
Associated Insertion(s)
Carried in Construct
P{35UZ}
Also Known As
Ubx-lacZ
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Irvine et al., 1993)
Progenitor genotype
Carried in construct
P{35UZ}
Cytology
Description

lacZ expression is governed by 35.4kb from the upstream control region of Ubx, extending from the start of transcription in a 5' direction.

(Irvine et al., 1991)
Allele components
Component
Use(s)
Encoded product / tool
lacZ
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage
      parasegment 6 -- parasegment 12 of larval ventral nerve cord
      (Matthews, 1995.11.17)
      epidermis of parasegment 6 -- parasegment 12
      (Matthews, 1995.11.17)
      embryonic/larval midgut of parasegment 7
      (Matthews, 1995.11.17)
      embryonic stage 5
      parasegment 6
      (Irvine et al., 1991)
      embryonic stage 7
      parasegment 6 & parasegment 10 & parasegment 12
      (Irvine et al., 1991)
      embryonic stage 8
      parasegment 6 & parasegment 8 & parasegment 10 & parasegment 12
      (Irvine et al., 1991)
      lateral ectoderm of parasegment 8 -- parasegment 12
      (Irvine et al., 1991)
      embryonic stage 9
      parasegment 5 -- parasegment 12
      (Irvine et al., 1991)
      embryonic stage 9 -- 17
      head
      (Irvine et al., 1991)
      embryonic stage 10 -- 17
      lateral ectoderm of parasegment 5 -- parasegment 13
      (Irvine et al., 1991)
      embryonic stage 11 -- 17
      somatic mesoderm of parasegment 6 -- parasegment 12
      (Irvine et al., 1991)
      embryonic stage 12 -- 17
      anal pad
      (Irvine et al., 1991)
      embryonic/larval hindgut
      (Irvine et al., 1991)
      embryonic stage 14 -- 15
      parasegment 7 of presumptive embryonic/larval central nervous system
      (Irvine et al., 1991)
      parasegment 8 of presumptive embryonic/larval central nervous system
      (Irvine et al., 1991)
      parasegment 9 of presumptive embryonic/larval central nervous system
      (Irvine et al., 1991)
      parasegment 10 of presumptive embryonic/larval central nervous system
      (Irvine et al., 1991)
      parasegment 11 of presumptive embryonic/larval central nervous system
      (Irvine et al., 1991)
      parasegment 12 of presumptive embryonic/larval central nervous system
      (Irvine et al., 1991)
      parasegment 6 of presumptive embryonic/larval central nervous system
      (Irvine et al., 1991)
      embryonic stage 14 -- 17
      visceral mesoderm of parasegment 7
      (Irvine et al., 1991)
      third instar larval stage
      metathoracic leg disc | posterior
      (Irvine et al., 1991)
      haltere disc | posterior
      (Irvine et al., 1991)
      wing disc posterior compartment
      (Docquier et al., 1999)
      haltere disc posterior compartment
      (Docquier et al., 1999)
      leg disc posterior compartment
      (Docquier et al., 1999)
      Additional Information
      Statement
      Reference

      In embryos, EcollacZ expression closely mimics that of Ubx transcripts. Earliest detectable stage (stage 5) corresponds to that observed for the Ubx mRNA, as opposed to that of the Ubx protein, which cannot be detected until stage 9. Ectopic expression in head and tail reigons observed.In late embryos, differs from wild-type Ubx in that expression in CNS of parasegment 5 is not observed.In metathoracic discs, expression in regions corresponding to parasegment 5 (anterior) is not observed.

      (Irvine et al., 1991)
       
      Marker for
      Reflects expression of
      Ubx
      (Docquier et al., 1999, Matthews, 1995.11.17, Irvine et al., 1991)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Overexpression of Ubx via a heat shock promoter represses Ecol\lacZ ("Ubx") expression.

      (Irvine et al., 1993)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (22)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Presence of trx is able to maintain long term Ubx expression.

      (Chinwalla et al., 1995)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      35UZ
      (Hacohen et al., 1998)
      Ecol\lacZUbx.35UZ
      lacZUbx.35UZ
      Name Synonyms
      Secondary FlyBase IDs
        References (19)