FB2024_03 , released June 25, 2024
Allele: Dmel\esgG66
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General Information
Symbol
Dmel\esgG66
Species
D. melanogaster
Name
FlyBase ID
FBal0039323
Feature type
allele
Associated gene
Dmel\esg
Associated Insertion(s)
P{enG}esgG66
Carried in Construct
Also Known As
esgG66B, esg-lacZ
Key Links
Allele class

amorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - genetic evidence

(Fuse et al., 1996, Samakovlis et al., 1996, Tanaka-Matakatsu et al., 1996)
Mutagen
Delta2-3 transposase
(Kassis, 1994, Whiteley et al., 1992)
Progenitor genotype
P{enG}6
(Kassis, 1994, Whiteley et al., 1992)
Associated Insertion(s)
P{enG}esgG66
Cytology
Description

The 3.5kb of esg sequences between esg coordinates 680 and 4200 have been deleted. A copy of the P{enG} transposon of the progenitor has flipped orientation and is now inserted at esg coordinate 4200.

(Kassis, 1994)

Contains the P{enG} transposon in the opposite orientation from the progenitor, downstream of the esg coding region. Approximately 3kb DNA of esg coding sequence between nucleotides 800 and 4100 has been deleted.

(Whiteley et al., 1992)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage 17
      gonadal sheath proper primordium
      (Le Bras and Van Doren, 2006)
      adult stage
      hub cell
      (Le Bras and Van Doren, 2006)
      Additional Information
      Statement
      Reference

      Expressed in a tight cluster of somatic gonadal precursor cells at the anterior of embryonic stage 17 male gonads. It is also expressed in the adult hub. It is not expressed in female gonads.

      (Le Bras and Van Doren, 2006)
       
      Marker for
      Reflects expression of
      esg
      (Le Bras and Van Doren, 2006)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      No defects in dendrite pruning of ddaC dorsal dendritic arborising neurons are seen in homozygous clones at 18 hours after puparium formation.

      (Loncle and Williams, 2012)

      2o and 3o pigment cells in esgG66 clones of the eye form a cell lattice at ~36 hours APF as occurs in wild-type eyes. However, the cells in the clone fail to undergo the apical restriction that usually follows the lattice stage. Cells within the clone show no obvious defects in actin cytoskeleton or cellular morphology.

      esgG66 clones in the adult eye show a dramatic loss of 2o and 3o pigment cells resulting in gaps between ommatidia.

      (Lim and Tomlinson, 2006)

      Transplantation experiments in which prospective germ cells from homozygous esgG66 embryos are transplanted into agametic hosts show that esg is dispensible for gametogenesis in both the male and the female germline as fertile male and female host animals which have integrated homozygous esgG66 donor germ cells have been recovered.

      (Streit et al., 2002)

      The size of the primary branches of the tracheal system is largely unaffected in mutant embryos, even though segments of the dorsal trunk are disconnected.

      (Beitel and Krasnow, 2000)

      Homozygous clones induced in the leg disc at the late second larval instar stage are sometimes associated with ectopic fold formation.

      (Goto and Hayashi, 1999)

      The dorsal branch fusion cells form discrete branches that never meet or join. These cells go on to form additional branches that resemble normal terminal branches in the larva. Lateral trunk fusion branches are completely missing leaving a gap in each segment of the lateral trunk.

      (Samakovlis et al., 1996)

      Failure of tracheal fusion in embryos. Tip cells do not accumulate shg in their filopodia, which migrate in other directions and no fusion occurs. Cell motility is hyperactivated in tip cells of both thin and thick tracheal branches. esgG66 P{HS-esg.F} embryos heat shocked seven times exhibit restoration of shg expression and adhesion, the trachea completely fuse.

      (Tanaka-Matakatsu et al., 1996)

      10-50% of homozygotes die as embryos, the rest die as larvae. esgG66/esgG66-R flies are viable.

      (Whiteley et al., 1992)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      esgG66, sna1 clones that express both wordsRNA.842.Scer\UAS and wordsRNA.1701.Scer\UAS, under the control of Scer\GAL4Act5C.PP, in the eye show ectopic photoreceptor and cone cells and a significant reduction of the pigment rim.

      esgG66, sna1 clones show the same loss of 2o and 3o pigment cells as esgG66 clones. This phenotype is not enhanced in esgG66, sna1 Scer\GAL4Act5C.PP>wordsRNA.842.Scer\UAS, wordsRNA.1701.Scer\UAS clones.

      (Lim and Tomlinson, 2006)

      The wing disc is absent in esgG66, sna1 double mutants, and the apical constrictions do not occur in the wing primordium cells. The haltere discs are similarly affected, but leg discs are unaffected. There is no sign of cell death in the prospective wing disc of the double mutant embryo. The mutant phenotypes can be rescued by either snahs.PF or esghs.PF.

      (Fuse et al., 1996)

      shghs.PU; esgG66 embryos heat shocked twice also exhibit fused trachea.

      (Tanaka-Matakatsu et al., 1996)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      esgG66 is rescued by esghs.PF

      (Fuse et al., 1996)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Excision of the P{enG} element can revert the mutant phenotype.

      (Ashburner et al., 1999)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      References (29)