FlyBase Allele Report: Dmel\dx[hs.PB]

General Information

Symbol

Dmel\dx^hs.PB

Species

D. melanogaster

Name

heat shock construct of Busseau

FlyBase ID

FBal0039305

Feature type

allele

Associated gene

Dmel\dx

Associated Insertion(s)

Carried in Construct

P{Hsp70-dx}

Also Known As

hs-dx

Key Links

Transgenic product class

cDNA

(Busseau et al., 1994)

wild_type

(Busseau et al., 1994)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Busseau et al., 1994)

wild_type

(Busseau et al., 1994)

Mutagen

in vitro construct

(Matsuno et al., 1995, Busseau et al., 1994)

Progenitor genotype

Carried in construct

P{Hsp70-dx}

Cytology

Description

A dx cDNA extending from 33 nucleotides 5' of the translation start site to a sole SpeI site within the 3' non-coding region expressed from an Hsp70 promoter.

(Busseau et al., 1994)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible

(Nagel et al., 2000, Matsuno et al., 1995)

Phenotype Manifest In

eo neuron

(Nagel et al., 2000)

macrochaeta

(Nagel et al., 2000)

ommatidium

(Matsuno et al., 1995)

thecogen cell | ectopic

(Nagel et al., 2000)

thorax & macrochaeta

(Matsuno et al., 1995)

tormogen cell

(Nagel et al., 2000, Matsuno et al., 1995)

tormogen cell | ectopic

(Nagel et al., 2000)

trichogen cell

(Nagel et al., 2000)

wing vein

(Matsuno et al., 1995)

Detailed Description

Statement

Reference

Overexpression of dx^hs.PB shortly after pupariation results in nearly bald flies. Remaining bristles show the normal set of external cells. Overexpression at later stages causes the formation of double sockets, also a cell fate change of the neuron into a sheath cell is observed.

(Nagel et al., 2000)

Induces a complex array of mutant phenotypes affecting eye, wing and bristle morphology. Overexpression between 0-24 hours after puparium formation causes a rough eye phenotype characterised by irregular ommatidia and lens disruptions indicative of cell death. Overexpression during the first six hours of pupariation results in loss of external sensory organ structures on the thorax giving a bald phenotype. Expression 24 hours after pupariation results in a 'double socket' and 'double shaft/single socket' phenotypes. Overexpression at late third instar or prepupal stages causes an extra bristle phenotype. Expression 0-24 hours after pupariation causes wing vein gaps, expression 24-48 hours after pupariation causes thickened veins.

(Matsuno et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

dx^hs.PB is a non-enhancer of visible phenotype of H^hs.PM

(Nagel et al., 2000)

NOT Suppressor of

Statement

Reference

dx^hs.PB is a non-suppressor of visible phenotype of H^hs.PM

(Nagel et al., 2000)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

dx^hs.PB is a non-enhancer of macrochaeta phenotype of H^hs.PM

(Nagel et al., 2000)

NOT Suppressor of

Statement

Reference

dx^hs.PB is a non-suppressor of macrochaeta phenotype of H^hs.PM

(Nagel et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Carried in plasmid "pCaSpeRhs-dx", expressed in S2 cells and used in a colocalisation assay of N and dx proteins.

(Matsuno et al., 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

dx^hs.PB

Name Synonyms

heat shock construct of Busseau

(Busseau et al., 1994)

Secondary FlyBase IDs

References (5)

Report Sections

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