FB2024_03 , released June 25, 2024
Allele: Dmel\abd-A1dB5.UAS
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General Information
Symbol
Dmel\abd-A1dB5.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0039121
Feature type
allele
Associated gene
Dmel\abd-A
Associated Insertion(s)
Carried in Construct
P{UAS-abd-A.M}
Also Known As
UAS-abd-A, UAS-abdA
Key Links
Transgenic product class
cDNA
(Michelson, 1994)
wild_type
(Michelson, 1994)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Michelson, 1994)
wild_type
(Michelson, 1994)
Mutagen
in vitro construct
(Bilder and Scott, 1998, Michelson, 1994)
Progenitor genotype
Carried in construct
P{UAS-abd-A.M}
Cytology
Description

abd-A cDNA expressed under influence of UASt regulatory sequences.

(Michelson, 1994)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
abd-A
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      abdominal segment 1, with Scer\GAL4C-765, Scer\GAL4c739

      (Casares et al., 1996)

      abdominal segment | ectopic, with Scer\GAL4C-765, Scer\GAL4pnr-MD237

      (Casares et al., 1996)

      abdominal segment | ectopic | posterior, with Scer\GAL4C-765, Scer\GAL4pnr-MD237

      (Casares et al., 1996)

      antenna, with Scer\GAL4dpp.blk1

      (Yao et al., 1999)

      antenna, with Scer\GAL4upd1-E132

      (Casares et al., 1996)

      aorta primordium, with Scer\GAL4how-24B

      (Lovato et al., 2002)

      aorta primordium, with Scer\GAL4twi.PG

      (Lovato et al., 2002)

      dorsal acute muscle cell | ectopic, with Scer\GAL4how-24B

      (Enriquez et al., 2010)

      embryo | dorsal closure stage, with Scer\GAL469B

      (Lovato et al., 2002)

      embryonic/larval oenocyte | ectopic, with Scer\GAL4ato.3.6

      (Brodu et al., 2002)

      embryonic/larval oenocyte | ectopic, with Scer\GAL4en-e16E

      (Brodu et al., 2002)

      embryonic/larval ostium | ectopic, with Scer\GAL4twi.PG

      (Lovato et al., 2002)

      embryonic/larval ostium | embryonic stage, with Scer\GAL4svp.PT

      (Trujillo et al., 2016)

      embryonic abdominal segment 1 & somatic muscle, with Scer\GAL4how-24B

      (Michelson, 1994)

      embryonic dorsal vessel, with Scer\GAL469B

      (Lovato et al., 2002)

      embryonic dorsal vessel, with Scer\GAL4how-24B

      (Lovato et al., 2002)

      embryonic dorsal vessel, with Scer\GAL4twi.PG

      (Lovato et al., 2002)

      embryonic mesothoracic segment, with Scer\GAL4ato.3.6

      (Brodu et al., 2002)

      embryonic mesothoracic segment, with Scer\GAL4en-e16E

      (Brodu et al., 2002)

      embryonic metathoracic segment, with Scer\GAL4ato.3.6

      (Brodu et al., 2002)

      embryonic metathoracic segment, with Scer\GAL4en-e16E

      (Brodu et al., 2002)

      embryonic prothoracic segment, with Scer\GAL4ato.3.6

      (Brodu et al., 2002)

      embryonic prothoracic segment, with Scer\GAL4en-e16E

      (Brodu et al., 2002)

      embryonic thoracic segment & somatic muscle, with Scer\GAL4how-24B

      (Michelson, 1994)

      embryonic visceral muscle cell | increased number, with Scer\GAL4how-24B

      (LaBeau et al., 2009)

      heart primordium, with Scer\GAL4how-24B

      (Lovato et al., 2002)

      heart primordium, with Scer\GAL4twi.PG

      (Lovato et al., 2002)

      leg | ectopic, with Scer\GAL4upd1-E132

      (Casares et al., 1996)

      metathoracic laterotergite, with Scer\GAL4C-765, Scer\GAL4pnr-MD237

      (Casares et al., 1996)

      muscle cell of A1-7 ventral acute muscle 1 | ectopic | precursor, with Scer\GAL4how-24B

      (Michelson, 1994)

      muscle cell of A2-7 ventral transverse muscle 1 | ectopic, with Scer\GAL4how-24B

      (Michelson, 1994)

      muscle cell of ventral oblique muscle 6 | ectopic, with Scer\GAL4how-24B

      (Michelson, 1994)

      neuroblast, with Scer\GAL4elav-C155

      (Maurange et al., 2008)

      neuroblast | somatic clone, with Scer\GAL4αTub84B.PL

      (Bello et al., 2007)

      scutum, with Scer\GAL4C-765, Scer\GAL4pnr-MD237

      (Casares et al., 1996)

      thoracic neuroblast | larval stage, with Scer\GAL4αTub84B.PL

      (Bello et al., 2003)

      thoracic neuroblast | larval stage, with Scer\GAL4αTub84B.PL, Scer\GAL4elav-C155

      (Bello et al., 2003)

      wing, with Scer\GAL4Bx-MS1096, Scer\GAL4C-765

      (Casares et al., 1996)
      Detailed Description
      Statement
      Reference

      Expression of abd-A1dB5.Scer\UAS driven by Scer\GAL4svp.PT causes ostia cells to be more elongated; this effect is even stronger when driven by Scer\GAL4twi.PG.

      (Trujillo et al., 2016)

      Scer\GAL4how-24B-mediated expression of abd-A1dB5.Scer\UAS results in an ectopic DA3 muscle in thoracic segment 1. The number of nuclei in this ectopic muscle is identical to that in wild type abdominal segments.

      (Enriquez et al., 2010)

      Embryos ectopically expressing Scer\GAL4how-24B driven abd-A1dB5.Scer\UAS throughout the mesoderm show an increased number of alary muscle pairs, from the seven normally observed in wild-type, to approximately ten. The additional muscles are located in the more anterior region.

      (LaBeau et al., 2009)

      Expression of abd-A1dB5.Scer\UAS under the control of Scer\GAL4elav-C155 results in neuroblast apoptosis in the thorax and abdomen in larvae at 96 hours.

      (Maurange et al., 2008)

      Expression of abd-A1dB5.Scer\UAS under the control of Scer\GAL4αTub84B.PL in clones in the larval central nervous system results in clones that are smaller than wild-type controls and which comprise only small cells (neuroblasts are lacking).

      (Bello et al., 2007)

      Postembryonic neuroblast (pNB) clones in the larval thorax expressing abd-A1dB5.Scer\UAS under the control of Scer\GAL4elav-C155 and Scer\GAL4αTub84B.PL are reduced in size compared to wild-type clones (from 58 to 20 neurons). In most of the mutant clones, no identifiable neuroblast is visible at 96 hours after larval hatching. Cells in the clones are always located close to the neuropil and have a morphology typical of early-born neurons. Postembryonic neuroblast (pNB) clones in the larval thorax expressing abd-A1dB5.Scer\UAS under the control of Scer\GAL4αTub84B.PL are reduced in size compared to wild-type clones and the clones show loss of the pNB in at least 82% of cases.

      (Bello et al., 2003)

      Expression of abd-A1dB5.Scer\UAS under the control of Scer\GAL4en-e16E results in the formation of ectopic oenocytes in the T1-T3 segments (they are normally only found in segments A1-A7). Expression of abd-A1dB5.Scer\UAS under the control of Scer\GAL4ato.3.6 results in the formation of ectopic oenocytes in the T1-T3 segments. Expression of abd-A1dB5.Scer\UAS under the control of Scer\GAL4salm-459.2 does not result in the formation of ectopic oenocytes in the T1-T3 segments.

      (Brodu et al., 2002)

      Embryos expressing abd-A1dB5.Scer\UAS under the control of Scer\GAL469B do not undergo dorsal closure to form a linear heart tube, although heart and aorta cells can still be distinguished. Expression of abd-A1dB5.Scer\UAS under the control of Scer\GAL4how-24B or Scer\GAL4twi.PG results in transformation of the aorta into heart. The lumen of the dorsal vessel is wider than normal. Variations in dorsal vessel diameter similar to that normally found in the heart are seen in anterior locations in embryos expressing abd-A1dB5.Scer\UAS under the control of Scer\GAL4twi.PG and occasionally tiny perforations in the heart wall are seen, consistent with the interpretation that ectopic ostia are being formed in anterior locations.

      (Lovato et al., 2002)

      Scer\GAL4E132 induced expression in the eye-antennal disc causes transformation of the antenna into leg. Scer\GAL4Bx-MS1096 or Scer\GAL4C-765 induced expression in the wing disc causes transformation of the wing into haltere-like tissue. Scer\GAL4C-765 and Scer\GAL4pnr-MD237 induced expression also transforms the mesonotum and metanotum into an abdominal segment. Scer\GAL4c739 and Scer\GAL4C-765 induced expression in the abdomen transforms A1 into a posterior abdominal segment.

      (Casares et al., 1996)

      When expressed via Scer\GAL4how-24B in embryos, an ectopic precursor for abdominal muscle 26 appears in the ventral region of T1-T3. Ventral longitudinal muscles of thoracic segments assume the identity of the abdominal segments. Abdominal segment 1 often gain two muscles that it normally lacks: external ventral oblique muscles 17 and 25. Some atypical muscles appear in T1 and T2. Insertion sites of transformed muscles are sometimes aberrant. The thoracic cuticle remains unaffected. There is no affect on the development of the gut.

      (Michelson, 1994)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The neuroblast apoptosis seen in the thorax and abdomen of 96 hour larvae expressing abd-A1dB5.Scer\UAS under the control of Scer\GAL4elav-C155 is suppressed by co-expression of casScer\UAS.cKa.

      (Maurange et al., 2008)

      Expression of abd-A1dB5.Scer\UAS results in an almost complete rescue of the transformed haltere phenotype of Ubxabx-1 Ubxbx-3 Ubxpbx-1/UbxLDN flies. The P{GawB}UbxLDN insertion within the UbxLDN flies drives expresssion of abd-A1dB5.Scer\UAS.

      Expression of abd-A1dB5.Scer\UAS cannot suppress the absence of genitalia in Abd-BLDN/Abd-BM1 female flies, not the presence of a large seventh tergite in both male and female Abd-BLDN/Abd-BM1 flies. abd-A1dB5.Scer\UAS expression is driven by the P{GawB}Abd-BLDN insertion in the Abd-BLDN flies.

      (de Navas et al., 2006)

      Postembryonic neuroblast (pNB) clones in the larval thorax expressing abd-A1dB5.Scer\UAS under the control of Scer\GAL4elav-C155 and Scer\GAL4αTub84B.PL and that are also mutant for Df(3L)H99 continue to proliferate throughout larval life and adopt the large size typical of wild-type lineages in the thorax. All these mutant clones retain their pNB at 96 hours after larval hatching.

      (Bello et al., 2003)

      Co-expression of hthScer\UAS.cPa partially suppresses the antenna to leg transformation phenotypes produced by ectopic expression of abd-A1dB5.Scer\UAS driven by Scer\GAL4dpp.blk1. The antennae produced are aristaless and occasionally duplicated, similar to the hthScer\UAS.cPa phenotype.

      (Yao et al., 1999)

      Co-expression of exdScer\UAS.cGa (induced by Scer\GAL4Bx-MS1096) does not significantly modify the wing transformation. Ubxabx-1 Ubxbx-3 Ubxpbx-1/Ubxlac1 combination transforms the metathorax into mesothorax (including transformation of halteres into wings), Scer\GAL4Bx-MS1096 induced expression allows full rescue.

      (Casares et al., 1996)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Expression dependent on Scer\GAL4.

      (Michelson, 1994)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      abd-A1dB5.Scer\UAS
      abd-A1dB5.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (21)