FB2024_03 , released June 25, 2024
Allele: Dmel\Adh+t3.2
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General Information
Symbol
Dmel\Adh+t3.2
Species
D. melanogaster
Name
FlyBase ID
FBal0038104
Feature type
allele
Associated gene
Dmel\Adh
Associated Insertion(s)
Carried in Construct
P{1010}
P{HS22}
P{HS24}
Key Links
Genomic Maps

Transgenic product class
genomic_DNA
(Posakony et al., 1985)
wild_type
(Posakony et al., 1985)
Mutagen
Nature of the Allele
Transgenic product class
genomic_DNA
(Posakony et al., 1985)
wild_type
(Posakony et al., 1985)
Mutagen
in vitro construct
(Kirkpatrick et al., 1994, Ayer and Benyajati, 1992, Kirkpatrick and Martin, 1992, Sass, 1990, Wilson et al., 1989, Pirrotta, 1988, Martin et al., 1986, Posakony et al., 1985)
Progenitor genotype
Adh+t11.8
(Posakony et al., 1985)
Carried in construct
P{1009}
P{1010}
P{Adh3.2XbaI}
P{Adh3.2A}
P{Adh3.2-ry}
P{HS22}
P{HS24}
P{PA-1}
P{PA-2}
Cytology
Description

A 3.2kb XbaI fragment that carries the AdhF allele.

(Pirrotta, 1988)

3.2kb XbaI Adh fragment which has 0.66kb of 5' flanking sequences and 0.64kb of 3' flanking sequences, all of the cis-acting DNA sequences necessary for the major tissue specificities of Adh expression.

(Posakony et al., 1985)
Allele components
Component
Use(s)
Regulatory region(s)
Adh
Encoded product / tool
Adh
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
rescue_region
2L:14,614,895..14,618,070 [+]
Linked to:
XbaI-XbaI restriction fragment
Comment:

The 3.2kb XhoI rescue fragment contains all of the cis-acting DNA sequences necessary for the major tissue specificities of Adh expression; position of restriction fragment on reference sequence inferred by FlyBase curator.

(Posakony et al., 1985)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Adh expression has correct tissue specificity but Adh protein levels of adult flies is reduced by two fold and levels in third instar larval by 10-fold.

      (Posakony et al., 1985)

      Carried in a plasmid, expressed in E.coli cells and injected into preblastoderm embryos. Larvae and adults developed from these embryos are dissected and stained for Adh activity.

      (Martin et al., 1986)

      Carried in a plasmid and used in a transient expression assay.

      (Kirkpatrick et al., 1994)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Adh+t3.2
      Name Synonyms
      Secondary FlyBase IDs
      • FBal0033362
      References (25)