FlyBase Allele Report: Dmel\nanos[αTub84B.UTR.Tag:HA]

General Information

Symbol

Dmel\nanos^{αTub84B.UTR.Tag:HA}

Species

D. melanogaster

Name

FlyBase ID

FBal0035300

Feature type

allele

Associated gene

Dmel\nanos

Associated Insertion(s)

Carried in Construct

P{nanos-tub3'UTR.w}

P{nanos-tub3'UTR}

Also Known As

nos-tub3'UTR, nos-tub3''UTR

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Gavis and Lehmann, 1994)

Progenitor genotype

Carried in construct

P{nanos-tub3'UTR.w}

P{nanos-tub3'UTR}

Cytology

Description

Genomic nos fragment in which the nos 3'UTR (834bp EcoRI-XhoI fragment) has been replaced with the αTub84B 3'UTR (197bp ScaI-HpaI fragment). A Tag:HA epitope has been inserted at the 5' end of the nos coding region.

(Gavis and Lehmann, 1994)

Allele components

Component

Use(s)

Regulatory region(s)

nanos

Encoded product / tool

nanos

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage

(Brechbiel and Gavis, 2008)

female sterile | dominant

(Arrizabalaga and Lehmann, 1999, Gavis and Lehmann, 1994)

lethal - all die during embryonic stage | dominant | maternal effect

(Arrizabalaga and Lehmann, 1999)

some die during embryonic stage | dominant | maternal effect | maternal effect

(Arrizabalaga and Lehmann, 1999)

Phenotype Manifest In

embryonic head

(Crucs et al., 2000)

embryonic head | maternal effect

(Arrizabalaga and Lehmann, 1999)

embryonic thoracic segment

(Crucs et al., 2000)

embryonic thoracic segment | maternal effect

(Arrizabalaga and Lehmann, 1999)

larval multidendritic class IV neuron | third instar larval stage

(Brechbiel and Gavis, 2008)

Detailed Description

Statement

Reference

Third instar larvae expressing nos^{αTub84B.UTR.T:Ivir\HA1} show a significant reduction in terminal dendritic branching of the class IV da neurons.

(Brechbiel and Gavis, 2008)

Embryos derived from nos^BN females that also carry nos^{αTub84B.UTR.T:Ivir\HA1} lack all head and thoracic segments.

(Crucs et al., 2000)

Females carrying nos^{αTub84B.UTR.T:Ivir\HA1} are completely sterile, producing embryos with head defects and lack of thoracic segments.

(Arrizabalaga and Lehmann, 1999)

One copy of P{nos-tub3'UTR} produces a phenotype that resembles mislocalisation of nos mRNA, embryos fail to hatch, they lack anterior structures and often show duplication of posterior structures at the anterior.

(Gavis and Lehmann, 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressor of

Statement

Reference

nanos^{αTub84B.UTR.Tag:HA} is a suppressor of embryonic abdominal segment phenotype of vas¹

(Crucs et al., 2000)

nanos^{αTub84B.UTR.Tag:HA} is a suppressor of embryonic abdominal segment phenotype of vas³

(Crucs et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

The lack of abdominal segments seen in embryos derived from vas¹/vas³ females is rescued if the females also carry nos^{αTub84B.UTR.T:Ivir\HA1}.

(Crucs et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

nanos^{αTub84B.UTR.Tag:HA} partially rescues nanos^BN

(Crucs et al., 2000)

Comments

Embryos derived from nos^BN females carrying nos^{αTub84B.UTR.T:Ivir\HA1} show severe defects in germ cell migration and gonad formation.

(Gavis et al., 2008)

nos^{αTub84B.UTR.T:Ivir\HA1} rescues the abdominal segmentation defect in the posterior half of embryos derived from nos^BN females.

(Crucs et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

nanos^{αTub84B.UTR.Tag:HA}

nos^{αTub84B.UTR.T:Ivir\HA1}

nos^{αTub84B.UTR.Tag:HA}

nos^αTub84B.UTR

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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