FlyBase Allele Report: Dmel\Ras85D[Q13.hs]

General Information

Symbol

Dmel\Ras85D^Q13.hs

Species

D. melanogaster

Name

FlyBase ID

FBal0033979

Feature type

allele

Associated gene

Dmel\Ras85D

Associated Insertion(s)

Carried in Construct

P{hs-Ras1^Q13}

Key Links

Transgenic product class

missense_variant

(Lu et al., 1993)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Lu et al., 1993)

Mutagen

in vitro construct

(Reichman-Fried et al., 1994, Lu et al., 1993)

Progenitor genotype

Carried in construct

P{hs-Ras1^Q13}

Cytology

Description

Expression of an activated Ras85D is driven by an Hsp70 promoter.

(Reichman-Fried et al., 1994)

Hsp70 promoter drives expression of Ras85D that has been mutated to carry a G13Q amino acid replacement.

(Lu et al., 1993)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

Ras85D

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell death

(Kurada and White, 1998)

lethal - all die during embryonic stage | maternal effect

(Lu et al., 1993)

some die during embryonic stage | maternal effect

(Lu et al., 1993)

Phenotype Manifest In

border follicle cell

(Lee and Montell, 1997)

embryonic/first instar larval cuticle | dorsal

(Harden et al., 1999)

embryonic head | maternal effect

(Lu et al., 1993)

filzkorper | embryonic stage | maternal effect

(Lu et al., 1993)

Detailed Description

Statement

Reference

Expression of Ras85D^Q13.hs using heat shock results in a high frequency of defects in the dorsal cuticle.

(Harden et al., 1999)

Expression in the embryo inhibits, to large extent, naturally occurring cell death. Apoptosis only remains in the CNS.

(Kurada and White, 1998)

Partial rescue of tracheal migration defect in btl homozygotes.

(Reichman-Fried et al., 1994)

One copy of the P{hs-Ras1^Q13} construct causes a low hatching rate. Some unhatched embryos display a range of abdominal defects and duplicated filzkorper material. Other display defects in head involution, germband retraction or dorsal/ventral patterning.

(Lu et al., 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Ras85D^Q13.hs has border follicle cell phenotype, suppressible by slbo^UAS.cLa/Scer\GAL4^hs.PB

(Lee and Montell, 1997)

Additional Comments

Genetic Interactions

Statement

Reference

Border cell migration defect is rescued by Scer\GAL4^hs.PB-mediated expression of slbo^Scer\UAS.cLa.

(Lee and Montell, 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Lu.

(Reichman-Fried et al., 1994)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Ras1^Q13

(Lu et al., 1993)

Ras85D^Q13.hs

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

Open Close