FB2024_03 , released June 25, 2024
Allele: Dmel\dac1
Open Close
General Information
Symbol
Dmel\dac1
Species
D. melanogaster
Name
FlyBase ID
FBal0031014
Feature type
allele
Associated gene
Dmel\dac
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

loss of function allele

amorphic allele - genetic evidence

Mutagen
    Nature of the Allele
    Allele class

    amorphic allele - genetic evidence

    (Shen and Mardon, 1997, Mardon et al., 1994)

    loss of function allele

    (Martini et al., 2000)
    Mutagen
    Progenitor genotype
    Cytology
    Description

    Nucleotide substitution: C?T.

    (Tavsanli et al., 2004)

    Amino acid replacement: Q225term.

    (Tavsanli et al., 2004)
    Mutations Mapped to the Genome
    Curation Data
    Type
    Location
    Additional Notes
    References
    point_mutation
    2L:16,484,070..16,484,070 [-]
    Nucleotide change:

    C16484070T

    Reported nucleotide change:

    C?T

    Amino acid change:

    Q225term | dac-PA; Q225term | dac-PB; Q225term | dac-PC; Q225term | dac-PD; Q225term | dac-PF; Q225term | dac-PG

    Reported amino acid change:

    Q225term

    (Tavsanli et al., 2004)
    Variant Molecular Consequences
    Associated Sequence Data
    DDBJ / EMBL / GenBank
    DNA sequence
    Protein sequence
     
    UniProtKB/Swiss-Prot
      UniProtKB/TrEMBL
        Expression Data
        Reporter Expression
        Additional Information
        Statement
        Reference
         
        Marker for
        Reflects expression of
        Reporter construct used in assay
        Human Disease Associations
        Disease Ontology (DO) Annotations
        Models Based on Experimental Evidence ( 0 )
        Disease
        Evidence
        References
        Modifiers Based on Experimental Evidence ( 0 )
        Disease
        Interaction
        References
        Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
         
        Disease-implicated variant(s)
         
        Phenotypic Data
        Phenotypic Class
        Phenotype Manifest In

        adult mushroom body (with dac3)

        (Martini et al., 2000)

        adult mushroom body (with Df(2L)dac-4)

        (Martini et al., 2000)

        adult mushroom body alpha-lobe (with dac3)

        (Martini et al., 2000)

        adult mushroom body alpha-lobe (with Df(2L)dac-4)

        (Martini et al., 2000)

        adult mushroom body alpha-lobe | larval stage (with dac3)

        (Martini et al., 2000)

        adult mushroom body alpha-lobe | larval stage (with Df(2L)dac-4)

        (Martini et al., 2000)

        adult mushroom body beta'-lobe (with dac3)

        (Martini et al., 2000)

        adult mushroom body beta'-lobe (with Df(2L)dac-4)

        (Martini et al., 2000)

        adult mushroom body beta-lobe (with dac3)

        (Martini et al., 2000)

        adult mushroom body beta-lobe (with Df(2L)dac-4)

        (Martini et al., 2000)

        adult mushroom body gamma-lobe (with dac3)

        (Martini et al., 2000)

        adult mushroom body gamma-lobe (with dac3), with Scer\GAL4ey-OK107, dac7C1.UAS

        (Martini et al., 2000)

        adult mushroom body gamma-lobe (with Df(2L)dac-4)

        (Martini et al., 2000)

        adult mushroom body gamma-lobe (with Df(2L)dac-4), with Scer\GAL4ey-OK107, dac7C1.UAS

        (Martini et al., 2000)

        adult mushroom body gamma-lobe (with Df(2L)dac-4), with Scer\GAL4ey-OK107, dac21.UAS

        (Martini et al., 2000)

        calyx of adult mushroom body (with dac3)

        (Martini et al., 2000)

        calyx of adult mushroom body (with Df(2L)dac-4)

        (Martini et al., 2000)

        ellipsoid body (with dac3)

        (Martini et al., 2000)

        ellipsoid body (with Df(2L)dac-4), with Scer\GAL4ey-OK107, dac21.UAS

        (Martini et al., 2000)

        eye

        (Tavsanli et al., 2004, Mardon et al., 1994)

        eye-antennal disc

        (Mardon et al., 1994)

        eye disc morphogenetic furrow

        (Shen and Mardon, 1997, Mardon et al., 1994)

        lamina tangential neuron

        (Chotard et al., 2005)

        leg

        (Tavsanli et al., 2004, Mardon et al., 1994)

        leg disc

        (Mardon et al., 1994)

        mesothoracic femur

        (Mardon et al., 1994)

        mesothoracic metatarsus

        (Mardon et al., 1994)

        mesothoracic tarsal segment 2

        (Mardon et al., 1994)

        mesothoracic tarsal segment 3

        (Mardon et al., 1994)

        mesothoracic tibia

        (Mardon et al., 1994)

        metathoracic femur

        (Mardon et al., 1994)

        metathoracic metatarsus

        (Mardon et al., 1994)

        metathoracic tarsal segment 2

        (Mardon et al., 1994)

        metathoracic tarsal segment 3

        (Mardon et al., 1994)

        metathoracic tibia

        (Mardon et al., 1994)

        mushroom body spur (with dac3)

        (Martini et al., 2000)

        ommatidium

        (Mardon et al., 1994)

        photoreceptor cell R1

        (Mardon et al., 1994)

        photoreceptor cell R2

        (Mardon et al., 1994)

        photoreceptor cell R3

        (Mardon et al., 1994)

        photoreceptor cell R4

        (Mardon et al., 1994)

        photoreceptor cell R5

        (Mardon et al., 1994)

        photoreceptor cell R6

        (Mardon et al., 1994)

        photoreceptor cell R7

        (Mardon et al., 1994)

        photoreceptor cell R8

        (Mardon et al., 1994)

        prothoracic femur

        (Mardon et al., 1994)

        prothoracic metatarsus

        (Mardon et al., 1994)

        prothoracic tarsal segment 2

        (Mardon et al., 1994)

        prothoracic tarsal segment 3

        (Mardon et al., 1994)

        prothoracic tibia

        (Mardon et al., 1994)
        Detailed Description
        Statement
        Reference

        Although dac1 lamina precursor cells are able to undergo mitosis and are integrated into columns, they fail to express a late neuronal differentiation marker.

        (Chotard et al., 2005)

        Mutant animals have almost no eye tissue, and small fused legs.

        (Tavsanli et al., 2004)

        The mushroom bodies of dac1/dac3 or dac1/Df(2L)dac-4 adults show a number of defects. The α lobes are reduced in size (this phenotype is completely penetrant). Aberrant projections are often seen emanating from the calyx along the peduncle and in the direction of the tips of the α lobes. The medial (β, β' and γ) lobes appear grossly disorganised (this phenotype is completely penetrant). Aberrant projections are often seen emanating from the γ lobe and projections from the heel are occasionally seen in dac1/dac3 adults. The ellipsoid body is severely disorganised in dac1/dac3 adults. Only 3.2% of dac1/dac3 and 8.9% of dac1/Df(2L)dac-4 third instar larvae show a marked reduction in size of the α-type lobe and the defect is frequently unilateral. No aberrant projections are evident from the β-type lobe, the peduncle or the calyx.

        (Martini et al., 2000)

        Homozygous adults exhibit severely reduced or no eyes due to failure of the morphogenetic furrow to initiate.

        (Shen and Mardon, 1997)

        Eyes of homozygotes are severely reduced and roughened, or absent. Ommatidia have either too many or too few photoreceptors, and the ommatidial array is disrupted. Morphogenetic furrow movement does not occur in about half mutant eye discs, and is dramatically reduced in the rest. Mutants have short, little legs. Femur, tibia and proximal three tarsi are severely condensed. Cell death is increased in mutant leg discs.

        (Mardon et al., 1994)
        External Data
        Interactions
        Show genetic interaction network for Enhancers & Suppressors
        Phenotypic Class
        Phenotype Manifest In
        Additional Comments
        Genetic Interactions
        Statement
        Reference

        dac1; eyD1Da and dac1; eyJD double heterozygotes have normal mushroom bodies and eyes.

        (Martini et al., 2000)

        Modifies the EgfrE3 mutant eye phenotype.

        (Mardon et al., 1994)
        Xenogenetic Interactions
        Statement
        Reference
        Complementation and Rescue Data
        Rescued by

        dac1 is rescued by Scer\GAL4dpp.blk1/dacUAS.cSa

        (Shen and Mardon, 1997)
        Partially rescued by

        dac1/Df(2L)dac-4 is partially rescued by dac21.UAS/Scer\GAL4ey-OK107

        (Martini et al., 2000)

        dac1/Df(2L)dac-4 is partially rescued by Scer\GAL4ey-OK107/dac7C1.UAS

        (Martini et al., 2000)

        dac1/dac3 is partially rescued by Scer\GAL4ey-OK107/dac7C1.UAS

        (Martini et al., 2000)
        Comments

        dac7C1.Scer\UAS expressed under the control of Scer\GAL4OK107 rescues the mushroom body defects of dac1/dac3 or dac1/Df(2L)dac-4 animals to very close to wild type; the γ lobe shows minor disorganisation. The rescue is temperature dependent (greater rescue at 25oC compared to 18oC). dac21.Scer\UAS expressed under the control of Scer\GAL4OK107 rescues the mushroom body defects of dac1/Df(2L)dac-4 animals to very close to wild type; the γ lobe shows minor disorganisation. The rescue is temperature dependent (greater rescue at 25oC compared to 18oC). The ellipsoid body defects are not rescued.

        (Martini et al., 2000)

        Scer\GAL4dpp.blk1 mediated expression of dacScer\UAS.cSa rescues eye development (fully rescues the morphogenetic furrow initiation defect) but the ommatidial array is disorganised.

        (Shen and Mardon, 1997)
        Images (0)
        Mutant
        Wild-type
        Stocks (2)
        Notes on Origin
        Discoverer
        External Crossreferences and Linkouts ( 0 )
        Synonyms and Secondary IDs (1)
        Reported As
        Symbol Synonym
        dac1
        (Apitz and Salecker, 2015, Ahn et al., 2011, Salzer et al., 2010, Emmons et al., 2007, Chotard et al., 2005)
        Name Synonyms
        Secondary FlyBase IDs
          References (13)