FlyBase Allele Report: Dmel\bam[Δ86]

Clones of bam^Δ86 mutant female germline stem cells fail to properly differentiate, causing the accumulation of spectrosome-containing cells throughout the germarium.

(Dolezal et al., 2015)

bam^Δ59/bam^Δ86 adult females are completely sterile display tumorigenic phenotype in ovaries ('bag of marbles' phenotype). The full female sterility is not improved upon Wolbachia pipientis infection.

The completely sterility of bam^BG/bam^Δ86 males is not improved upon Wolbachia pipientis infection.

(Flores et al., 2015)

Expression of bam^hs.PO induced via heat shock in a bam^Δ86/bam^Δ86 background results in increased germline stem cell differentiation, as evidenced by a lack of germline stem cells 24 h after heat shock, and germaria exhibiting only fusome-containing cysts within the niche, and very few spectrosome-containing cells; however at 48 h after heat shock, a majority of germaria show some single spectrosome-containing cells.

(Liu et al., 2015)

Heterozygous bam^Δ86 testes show a ~60% 'tumor' rate.

(Chen et al., 2014)

Homozygous germline clones in the ovary result in stem cell-like tumours in the anterior region of the germarium. In contrast to wild type, the tumorous cells do not show a decrease in nucleolar size as the distance from the stem cell niche increases.

(Herzig et al., 2014)

In bam^z3-2884/bam^Δ86 mutant ovaries, the germaria and most of the egg chambers are filled with undifferentiated cystoblasts.

(Pan et al., 2014)

bam^Δ86/+ germaria have an increased number of spectrosome-containing cells compared to wild type.

(Cash and Andrews, 2012)

Approximately 27% of bam^Δ86/+ spermatocyte cysts contain 32 cells with 2% containing 64 cells.

(Monk et al., 2010)

75% of fusomes are spherical in bam^Δ86 ovaries, and the remaining 25% are short and cylindrical.

(Chau et al., 2009)

bam¹/bam^Δ86 mutant testes show large germline cysts containing 32 or more cells undergoing S-phase, some located far from the testis tip, indicating that more than the usual 4 rounds of transit amplification has occurred.

bam^Δ86/+ males usually make the transition from mitotic proliferation to spermatocyte differentiation, but often this occurs late. 43% of germline cysts complete at least one extra round of transit amplification prior to differentiation.

40% of male germline cysts prematurely differentiate at the 8-cell stage in males carrying one copy of bam^ΔPEST in a bam^Δ86/+ background. In these cases, the 8-cell cysts have large cells with typical spermatocyte morphology. Post-meiotic cysts are observed with 32 instead of 64 elongating spermatids.

(Insco et al., 2009)

Each bam^z3-2884/bam^Δ86 transheterozygous ovariole has a tumorous germarium with 0 or 1 normal egg chambers, in contrast to wild type ovarioles that have 7-10 egg chambers after the germarium.

In bam^z3-2884/bam^Δ86 ovarioles, the tumorous germaria and egg chambers contain many single germline stem cells.

bam^z3-2884 ovarioles carry a tumourous germarium and no normal egg chambers.

bam^Δ86/+ germaria show a signifiant increase in cystoblast number compared to controls.

(Shen et al., 2009)

Mutant germaria are tumorous, containing only undifferentiated germline stem cell-like single germ cells.

bam^Δ86 germline stem cell (GSC) clones outcompete normal, wild type GSCs and occupy their niches within germaria; in addition, mutant cystoblasts can invade the niche occupied by wild type GSCs and assume GSC identity. This appears to happen by the mutant GSCs simply forcing out the neighbouring wild type GSCs, as neither apoptosis nor differentiation is induced.

bam^Δ86 germline stem cells (GSCs) divide significantly faster than controls.

(Jin et al., 2008)

In bam^Δ86 homozygous females, there is a substantial increase in the numbers of both escort stems cells and escort cells per germarium.

(Decotto and Spradling, 2005)

In bam^Δ86 female mutants, the ovariole tip contains a "stem cell tumour", comprising hundreds of germline stem cells (GSCs). This is in sharp contrast to wild-type ovarioles, which contain only 2-3 GSCs. As with wild-type GSCs, bam^Δ86 GSCs delay cytokinesis. However, bam^Δ86 GSCs lack the associated cap cells seen in wild type. Additionally, the new fusome of bam^Δ86 GSCs grows to the same size as the old fusome and does not stretch out into the "exclamation point" configuration characteristic of GSCs in contact with cap cells.

(Kai et al., 2005)

Mutant females have tumorous ovarioles containing 300-1000 undifferentiated germ cells.

(Szakmary et al., 2005)

bam^Δ86 female germ cells fail to differentiate.

(Xi et al., 2005)

The cells of bam^Δ86/bam^Δ86 female germ-line cell clones do not contain round spectrosomes and do not form germline cysts.

(Casanueva and Ferguson, 2004)

bam^Δ86 homozygous females have ovaries with 'stem cell tumors' consisting of large numbers of undifferentiated germ cells.

(Gilboa et al., 2003)

Homozygous germ line clones in the testis overproliferate as spermatogonia-like cells.

(Shivdasani and Ingham, 2003)

bam^z3-2884/bam^Δ86 females are weakly fertile with small ovaries containing mostly wild-type egg chambers. Many germaria lack morphologically distinct cysts. Egg chambers containing endoreduplicating nurse cells and having a monolayer of follicle cells surrounding each egg chamber are seen.

(Ohlstein et al., 2000)

Homozygous ovarioles show a tumourous phenotype. No elongated spermatids are present in homozygous testes.

(Page et al., 2000)

Fate and proliferation of somatic cyst and hub cells is not affected in mutants testes. Mutant testes are not sexually transformed. Mutant clones induced in the cysts of male testes produce cysts with many more than 16 small germ cells, each having the morphological features of amplifying germ cells. Germ cells accumulating in the testes have a mixed identity, simultaneously retaining some aspects of stem cell fate while expressing markers of amplifying spermatogonial and primary spermatogonial cell fates. Distribution of both fusome and ring canal components indicates many mutant germ cells within a cyst are interconnected as a result of incomplete cytokinesis during the divisions leading to overproliferation. Most germ cells within a cyst undergo S and M phases in near-synchrony.

(Gonczy et al., 1997)

Homozygous females in which bam^hs.PO is expressed using heat shock begin to lay eggs within 7 days of heat shock. Some of these eggs hatch and produce viable, fertile adults. Rescued ovaries are smaller than wild-type and contain a mixture of normal and abnormal egg chambers. The females do not produce tumorous cysts once heat shocks are stopped.

(Ohlstein and McKearin, 1997)

Homozygotes exhibit tumorous egg chambers. bam^Δ86 enc^R1 double mutants also exhibit tumorous egg chambers.

(Hawkins et al., 1996)

Nearly every cell in an adult ovary contains a fusome, most often the organelles are spherical and sometimes appeared as two spheres that are close enough to touch. These look like a dumbbell and are suspected to be a fusome that has passed through a ring canal to connect two cells. Whenever a ring canal connects cells a fusome fills the canals lumen. bam^Δ86; hts¹ ovaries are phenotypically indistinguishable from bam^Δ86 ovaries.

(McKearin and Ohlstein, 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

bam^Δ86 has increased cell number | dominant phenotype, enhanceable by tut⁴

(Chen et al., 2014)

bam^Δ86 has abnormal cell cycle phenotype, enhanceable by Scer\GAL4^nanos.PG/how^L.UAS

(Monk et al., 2010)

bam^z3-2884/bam^Δ86 has female sterile phenotype, enhanceable by bgcn[+]/bgcn¹

(Ohlstein et al., 2000)

Suppressed by

Statement

Reference

bam^Δ86/bam^BG has male sterile phenotype, suppressible by Dsim\bam^bam.Venus

(Flores et al., 2015)

bam^Δ86/bam^Δ59 has female sterile phenotype, suppressible | partially by Dsim\bam^bam.Venus

(Flores et al., 2015)

bam^Δ86 has neoplasia | female | germline clone phenotype, suppressible by ball²

(Herzig et al., 2014)

bam^Δ86 has abnormal cell cycle phenotype, suppressible by how^stru-3R-3

(Monk et al., 2010)

bam^z3-2884/bam^Δ86 has female sterile phenotype, suppressible by eIF-4a[+]/eIF4A¹⁰⁰⁶

(Shen et al., 2009)

bam^z3-2884/bam^Δ86 has female sterile phenotype, suppressible by eIF-4a[+]/eIF4A¹⁰¹³

(Shen et al., 2009)

bam^z3-2884/bam^Δ86 has female sterile phenotype, suppressible by eIF-4a[+]/eIF4A^k01501

(Shen et al., 2009)

bam^z3-2884/bam^Δ86 has female sterile phenotype, suppressible by eIF-4a[+]/eIF4A⁰²⁴³⁹

(Shen et al., 2009)

bam^Δ86 has hyperplasia phenotype, suppressible by zpg^unspecified/zpg^unspecified

(Gilboa et al., 2003)

NOT suppressed by

Statement

Reference

bam^Δ86 has increased occurrence of cell division | germline clone | spermatogenesis phenotype, non-suppressible by put¹³⁵

(Shivdasani and Ingham, 2003)

Enhancer of

Statement

Reference

bam[+]/bam^Δ86 is an enhancer of increased cell number | adult stage | female | temperature conditional phenotype of Crei\I-CreI^hs.PR

(Ma et al., 2016)

bam[+]/bam^Δ86 is an enhancer of male semi-fertile phenotype of CHES-1-like^UAS.Tag:HA, Scer\GAL4^VP16.bam

(Yu et al., 2016)

bam[+]/bam^Δ86 is an enhancer of hyperplasia | adult stage | male phenotype of CHES-1-like^UAS.Tag:HA, Scer\GAL4^VP16.bam

(Yu et al., 2016)

bam[+]/bam^Δ86 is an enhancer of hyperplasia | adult stage | male phenotype of CHES-1-like^UAS.Tag:HA, Scer\GAL4^{VP16.nanos.UTR}

(Yu et al., 2016)

bam[+]/bam^Δ86 is an enhancer of increased cell number | recessive phenotype of tut⁴

(Chen et al., 2014)

bam[+]/bam^Δ86 is an enhancer of increased cell number phenotype of Scer\GAL4^VP16.bam, stg^UAS.cNa

(Insco et al., 2009)

Suppressor of

Statement

Reference

bam[+]/bam^Δ86 is a suppressor of decreased cell number phenotype of Scer\GAL4^VP16.bam, trbl^UASp.cMa

(Insco et al., 2009)

NOT Suppressor of

Statement

Reference

bam^Δ86 is a non-suppressor of decreased cell number | oogenesis phenotype of pelo¹

(Xi et al., 2005)

Other

Statement

Reference

CG6015^HMS01037, Scer\GAL4^bam.PU, bam^Δ86 has increased cell number | spermatogenesis phenotype

(Yu et al., 2021)

CG6015^HMS01037, Scer\GAL4^bam.PU, bam^Δ86 has increased occurrence of cell division | spermatogenesis phenotype

(Yu et al., 2021)

Dsor1^HMS00145, Scer\GAL4^bam.PU, bam^Δ86 has increased occurrence of cell division | spermatogenesis phenotype

(Yu et al., 2021)

Scer\GAL4^C587, bam^Δ86, bam^hs.PO, dpp^JF01371 has decreased cell number | heat sensitive phenotype

(Liu et al., 2015)

Scer\GAL4^C587, bam^Δ86, bam^hs.PO, ptc^JF03223 has decreased cell number | heat sensitive phenotype

(Liu et al., 2015)

bam[+]/bam^Δ86, mei-P26¹ has female semi-sterile phenotype

(Page et al., 2000)

bam^Δ86, mei-P26¹ has male sterile phenotype

(Page et al., 2000)

Phenotype Manifest In

Enhanced by

Statement

Reference

bam^Δ86 has testis phenotype, enhanceable by tut⁴

(Chen et al., 2014)

bam^z3-2884/bam^Δ86 has cystoblast | increased number phenotype, enhanceable by CSN5^N/CSN5[+]

(Pan et al., 2014)

bam^z3-2884/bam^Δ86 has cystoblast | increased number phenotype, enhanceable by Nedd8[+]/Nedd8^AN015

(Pan et al., 2014)

bam^Δ86 has spermatocyte cyst phenotype, enhanceable by Scer\GAL4^nanos.PG/how^L.UAS

(Monk et al., 2010)

bam^Δ86 has spermatocyte phenotype, enhanceable by Scer\GAL4^nanos.PG/how^L.UAS

(Monk et al., 2010)

bam^Δ86 has cystoblast | increased number phenotype, enhanceable by eIF4A^UASp.Tag:MYC/Scer\GAL4^{VP16.nanos.UTR}

(Shen et al., 2009)

bam^z3-2884/bam^Δ86 has phenotype, enhanceable by bgcn[+]/bgcn¹

(Ohlstein et al., 2000)

NOT Enhanced by

Statement

Reference

Dsim\bam^bam.Venus, bam^Δ86/bam^Δ59 has ovary phenotype, non-enhanceable by bgcn[+]/bgcn¹

(Flores et al., 2015)

bam^Δ86 has germline cyst | germline clone phenotype, non-enhanceable by Mad¹²

(Casanueva and Ferguson, 2004)

Suppressed by

Statement

Reference

bam^z3-2884/bam^Δ86 has cystoblast | increased number phenotype, suppressible by CSN4[+]/CSN4^N

(Pan et al., 2014)

bam^z3-2884/bam^Δ86 has cystoblast | increased number phenotype, suppressible by CSN4^k08018/CSN4[+]

(Pan et al., 2014)

bam^Δ86 has female germline cell phenotype, suppressible by udd¹

(Zhang et al., 2014)

bam^Δ86 has spermatocyte cyst phenotype, suppressible by how^stru-3R-3

(Monk et al., 2010)

bam^Δ86 has spermatocyte phenotype, suppressible by how^stru-3R-3

(Monk et al., 2010)

bam^z3-2884/bam^Δ86 has ovariole phenotype, suppressible by eIF-4a[+]/eIF4A¹⁰⁰⁶

(Shen et al., 2009)

bam^z3-2884/bam^Δ86 has ovariole phenotype, suppressible by eIF-4a[+]/eIF4A¹⁰¹³

(Shen et al., 2009)

bam^z3-2884/bam^Δ86 has ovariole phenotype, suppressible by eIF-4a[+]/eIF4A^k01501

(Shen et al., 2009)

bam^z3-2884/bam^Δ86 has ovariole phenotype, suppressible by eIF-4a[+]/eIF4A⁰²⁴³⁹

(Shen et al., 2009)

bam^z3-2884/bam^Δ86 has germarium phenotype, suppressible by eIF-4a[+]/eIF4A¹⁰⁰⁶

(Shen et al., 2009)

bam^z3-2884/bam^Δ86 has germarium phenotype, suppressible by eIF-4a[+]/eIF4A¹⁰¹³

(Shen et al., 2009)

bam^z3-2884/bam^Δ86 has germarium phenotype, suppressible by eIF-4a[+]/eIF4A^k01501

(Shen et al., 2009)

bam^z3-2884/bam^Δ86 has germarium phenotype, suppressible by eIF-4a[+]/eIF4A⁰²⁴³⁹

(Shen et al., 2009)

bam^z3-2884/bam^Δ86 has female germline stem cell | increased number phenotype, suppressible by eIF-4a[+]/eIF4A¹⁰⁰⁶

(Shen et al., 2009)

bam^z3-2884/bam^Δ86 has female germline stem cell | increased number phenotype, suppressible by eIF-4a[+]/eIF4A¹⁰¹³

(Shen et al., 2009)

bam^z3-2884/bam^Δ86 has female germline stem cell | increased number phenotype, suppressible by eIF-4a[+]/eIF4A^k01501

(Shen et al., 2009)

bam^z3-2884/bam^Δ86 has female germline stem cell | increased number phenotype, suppressible by eIF-4a[+]/eIF4A⁰²⁴³⁹

(Shen et al., 2009)

bam^z3-2884/bam^Δ86 has ovariole phenotype, suppressible by vas[+]/vas^RJ36

(Shen et al., 2009)

bam^z3-2884/bam^Δ86 has ovariole phenotype, suppressible by eIF4E1^S058911/eIF-4E[+]

(Shen et al., 2009)

bam^Δ86 has female germline stem cell | germline clone phenotype, suppressible by E2f1⁰⁷¹⁷²

(Jin et al., 2008)

bam^Δ86 has female germline stem cell | germline clone phenotype, suppressible by E2f1^rM729

(Jin et al., 2008)

bam^Δ86 has female germline stem cell phenotype, suppressible by pelo¹

(Xi et al., 2005)

bam^Δ86 has female germline stem cell phenotype, suppressible by zpg^unspecified/zpg^unspecified

(Gilboa et al., 2003)

NOT suppressed by

Statement

Reference

bam^Δ86 has female germline stem cell | increased number | somatic clone phenotype, non-suppressible by lili²

(Dolezal et al., 2015)

bam^Δ86 has female germline stem cell | increased number | somatic clone phenotype, non-suppressible by lili³

(Dolezal et al., 2015)

bam^Δ86 has germarium phenotype, non-suppressible by eIF-4a[+]/eIF4A¹⁰⁰⁶

(Shen et al., 2009)

bam^Δ86 has germarium phenotype, non-suppressible by eIF-4a[+]/eIF4A¹⁰¹³

(Shen et al., 2009)

bam^Δ86 has germarium phenotype, non-suppressible by eIF-4a[+]/eIF4A^k01501

(Shen et al., 2009)

bam^Δ86 has germarium phenotype, non-suppressible by eIF-4a[+]/eIF4A⁰²⁴³⁹

(Shen et al., 2009)

bam^Δ86 has ovariole phenotype, non-suppressible by eIF-4a[+]/eIF4A¹⁰⁰⁶

(Shen et al., 2009)

bam^Δ86 has ovariole phenotype, non-suppressible by eIF-4a[+]/eIF4A¹⁰¹³

(Shen et al., 2009)

bam^Δ86 has ovariole phenotype, non-suppressible by eIF-4a[+]/eIF4A^k01501

(Shen et al., 2009)

bam^Δ86 has ovariole phenotype, non-suppressible by eIF-4a[+]/eIF4A⁰²⁴³⁹

(Shen et al., 2009)

bam^Δ86 has female germline stem cell | germline clone phenotype, non-suppressible by put¹⁰⁴⁶⁰

(Jin et al., 2008)

bam^Δ86 has female germline stem cell | germline clone phenotype, non-suppressible by put¹³⁵

(Jin et al., 2008)

bam^Δ86 has female germline stem cell phenotype, non-suppressible by pelo[+]/pelo¹

(Xi et al., 2005)

bam^Δ86 has germline cyst | germline clone phenotype, non-suppressible by Mad¹²

(Casanueva and Ferguson, 2004)

bam^Δ86 has spermatogonium | increased number | germline clone phenotype, non-suppressible by put¹³⁵

(Shivdasani and Ingham, 2003)

bam^Δ86 has testis | germline clone phenotype, non-suppressible by put¹³⁵

(Shivdasani and Ingham, 2003)

Enhancer of

Statement

Reference

bam^Δ86 is an enhancer of male germline cell phenotype of CG6015^HMS01037, Scer\GAL4^bam.PU

(Yu et al., 2021)

bam^Δ86 is an enhancer of male germline cell phenotype of Dsor1^HMS00145, Scer\GAL4^bam.PU

(Yu et al., 2021)

bam^Δ86 is an enhancer of testis phenotype of Dhps^HMS02169, Scer\GAL4^bam.PU

(Chen et al., 2020)

bam^Δ86 is an enhancer of male fusome phenotype of Dhps^HMS02169, Scer\GAL4^bam.PU

(Chen et al., 2020)

bam^Δ86 is an enhancer of testis phenotype of Scer\GAL4^bam.PU, cnc^HMS00650

(Chen et al., 2020)

bam^Δ86 is an enhancer of testis phenotype of Scer\GAL4^bam.PU, cnc^HMS02021

(Chen et al., 2020)

bam[+]/bam^Δ86 is an enhancer of cystoblast | adult stage | increased number | temperature conditional phenotype of Crei\I-CreI^hs.PR

(Ma et al., 2016)

bam[+]/bam^Δ86 is an enhancer of testis | adult stage phenotype of CHES-1-like^UAS.Tag:HA, Scer\GAL4^VP16.bam

(Yu et al., 2016)

bam[+]/bam^Δ86 is an enhancer of testis | adult stage phenotype of CHES-1-like^UAS.Tag:HA, Scer\GAL4^{VP16.nanos.UTR}

(Yu et al., 2016)

bam[+]/bam^Δ86 is an enhancer of spermatid phenotype of CHES-1-like^UAS.Tag:HA, Scer\GAL4^{VP16.nanos.UTR}

(Yu et al., 2016)

bam[+]/bam^Δ86 is an enhancer of testis phenotype of tut⁴

(Chen et al., 2014)

bam[+]/bam^Δ86 is an enhancer of spermatogonium phenotype of tut⁴

(Chen et al., 2014)

bam[+]/bam^Δ86 is an enhancer of spermatogonial cyst phenotype of Scer\GAL4^VP16.bam, stg^UAS.cNa

(Insco et al., 2009)

bam[+]/bam^Δ86 is an enhancer of ovary phenotype of mei-P26¹

(Page et al., 2000)

Suppressor of

Statement

Reference

bam[+]/bam^Δ86 is a suppressor of egg chamber phenotype of aurB¹⁶⁸⁹

(Mathieu et al., 2013)

bam[+]/bam^Δ86 is a suppressor of germline cell phenotype of aurB¹⁶⁸⁹

(Mathieu et al., 2013)

bam[+]/bam^Δ86 is a suppressor of ring canal phenotype of aurB¹⁶⁸⁹

(Mathieu et al., 2013)

bam[+]/bam^Δ86 is a suppressor of nurse cell phenotype of aurB¹⁶⁸⁹

(Mathieu et al., 2013)

bam[+]/bam^Δ86 is a suppressor of female germline cyst phenotype of aurB¹⁶⁸⁹

(Mathieu et al., 2013)

bam[+]/bam^Δ86 is a suppressor of female fusome phenotype of aurB¹⁶⁸⁹

(Mathieu et al., 2013)

bam[+]/bam^Δ86 is a suppressor of spermatogonial cyst phenotype of Scer\GAL4^VP16.bam, trbl^UASp.cMa

(Insco et al., 2009)

bam^Δ86 is a suppressor of phenotype of CycA^Δ.Tag:HA

(Lilly et al., 2000)

Other

Statement

Reference

CG6015^HMS01037, Scer\GAL4^bam.PU, bam^Δ86 has spermatid phenotype

(Yu et al., 2021)

CG6015^HMS01037, Scer\GAL4^bam.PU, bam^Δ86 has male germline cell phenotype

(Yu et al., 2021)

CG6015^HMS01037, Scer\GAL4^bam.PU, bam^Δ86 has male fusome phenotype

(Yu et al., 2021)

CG6015^HMS01037, Scer\GAL4^bam.PU, bam^Δ86 has cyst cell of testis | increased number phenotype

(Yu et al., 2021)

Dsor1^HMS00145, Scer\GAL4^bam.PU, bam^Δ86 has male germline cell phenotype

(Yu et al., 2021)

Dsor1^HMS00145, Scer\GAL4^bam.PU, bam^Δ86 has male fusome phenotype

(Yu et al., 2021)

CHES-1-like^UAS.Tag:HA, Scer\GAL4^VP16.bam, bam[+]/bam^Δ86 has spermatid phenotype

(Yu et al., 2016)

Dsim\bam^bam.Venus, bam^Δ86/bam^Δ59 has ovary phenotype

(Flores et al., 2015)

Dsim\bam^bam.Venus, bam^Δ86/bam^Δ59 has female germline stem cell phenotype

(Flores et al., 2015)

Scer\GAL4^C587, bam^Δ86, bam^hs.PO, dpp^JF01371 has germarium | heat sensitive phenotype

(Liu et al., 2015)

Scer\GAL4^C587, bam^Δ86, bam^hs.PO, ptc^JF03223 has germarium | heat sensitive phenotype

(Liu et al., 2015)

ball², bam^Δ86 has female germline stem cell | germline clone phenotype

(Herzig et al., 2014)

CSN4^UASp.Tag:HA, Scer\GAL4^nanos.PU, bam[+]/bam^Δ86 has cystoblast | increased number phenotype

(Pan et al., 2014)

CSN5^N/CSN5[+], bam^Δ86 has cystoblast | increased number phenotype

(Pan et al., 2014)

Pdcd4¹, bam[+]/bam^Δ86 has germarium phenotype

(Cash and Andrews, 2012)

bam[+]/bam^Δ86, nanos^ALL.Tag:MYC has germarium phenotype

(Chau et al., 2012)

bam^Δ86, pum⁹/pum⁰²⁰⁰³ has ovary phenotype

(Chen and McKearin, 2005)

bam^Δ86, pum⁹/pum⁰²⁰⁰³ has germline cell | female phenotype

(Chen and McKearin, 2005)

bam^z3-2884/bam^Δ86, bgcn[+]/bgcn¹ has germline cell phenotype

(Ohlstein et al., 2000)

bam^z3-2884/bam^Δ86, bgcn¹ has germline cell phenotype

(Ohlstein et al., 2000)

bam^Δ86, mei-P26¹ has spermatid phenotype

(Page et al., 2000)

bam^Δ86, mei-P26¹ has egg chamber phenotype

(Page et al., 2000)

bam[+]/bam^Δ86, mei-P26¹ has egg chamber phenotype

(Page et al., 2000)

bam^Δ86, bgcn^QS2 has germline cell phenotype

(Gonczy et al., 1997)

Additional Comments

Genetic Interactions

Statement

Reference

The reduced male fertility as well as morphological defects in the adult testes (including but not limited to hyperplastic cyst overgrowth) observed in adult males expressing CHES-1-like^{Scer\UAS.T:Ivir\HA1} under the control of either the Scer\GAL4^{nos.UTR.T:Hsim\VP16} or the Scer\GAL4^{bam.T:Hsim\VP16} driver are enhanced to a variable degree by combination with a single copy of bam^Δ86.

(Yu et al., 2016)

Expression of dpp^JF01371 or ptc^JF03223, but not ci^GD1403, under the control of Scer\GAL4^C587 in flies expressing bam^hs.PO induced via heat shock in a bam^Δ86/bam^Δ86 background suppresses the recovery of spectrosome-containing cells at 48 h after heat shock seen in flies expressing bam^hs.PO alone in a bam^Δ86/bam^Δ86 background.

(Liu et al., 2015)

Introducing bam^Δ86/+ into the tut⁴ background blocks germline development at spermatogonial stage.

(Chen et al., 2014)

ball² bam^Δ86 double mutant germline cells in the ovary do not form tumours. The double mutant cells are lost from the stem cell niche, with the rate of stem cell loss being similar to that seen in ball² single mutants. Double mutant germline cells that have been displaced from the niche do not differentiate into germline cysts and they eventually degenerate.

(Herzig et al., 2014)

One copy of CSN4^N dramatically suppresses the increase in the number of undifferentiated cystoblasts seen in bam^z3-2884/bam^Δ86 mutants, dramatically increasing the number of differentiated cysts containing a branched fusome and the number of normal egg chambers per ovariole.

One copy of CSN4^k08018 dramatically suppresses the increase in the number of undifferentiated cystoblasts seen in bam^z3-2884/bam^Δ86 mutants, dramatically increasing the number of differentiated cysts containing a branched fusome and the number of normal egg chambers per ovariole.

Expression of CSN4^{Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^nos.PU in a bam^Δ86/+ mutant background results in an increase in cystoblast and two cell cyst number in the ovary compared to controls. Germline stem cell number is unaffected.

One copy of CSN5^N drastically and significantly enhances the differentiation defect of the bam^Δ86/+ mutants. The number of cystoblasts and two cell pairs is significantly increased compared to either heterozygote alone.

One copy of CSN5^N drastically enhances the differentiation defects seen in bam^z3-2884/bam^Δ86 mutant cystoblasts.

One copy of Nedd8^AN015 drastically enhances the differentiation defects seen in bam^z3-2884/bam^Δ86 mutant cystoblasts.

(Pan et al., 2014)

udd¹ partially suppresses the differentiation defects seen in bam^Δ86 mutant female germline cells. Many four- and eight-cell cysts with branched fusomes and ring canals are seen, but mature 16 cell cysts are not observed.

(Zhang et al., 2014)

The germarium phenotype of bam^Δ86 Df(3R)Exel6198/twin⁴¹ bam^Δ86 double mutant females is a mixture of a bam mutant phenotype (accumulation of germline stem cell (GSC)-like cells containing a spectrosome) and a twin mutant phenotype (lack of GSCs or lack of germ cells). The phenotype changes over time, with the percentage of bam mutant phenotype decreasing and the percentage of twin mutant phenotype increasing as the flies age. The double mutant GSCs are able to differentiate.

(Joly et al., 2013)

Removal of one copy of bam, through a bam^Δ86/+ strongly suppresses the aurB¹⁶⁸⁹ phenotype (from 17% to 4% of eight cell cysts), indicating that the phenotype is caused by the reduction of aurB activity in the cyst and not in the germline stem cell.

(Mathieu et al., 2013)

Pdcd4¹/Pdcd4¹, bam^Δ86/+ germaria contain significantly more spectrosome-containing cells compared to either Pdcd4¹/Pdcd4¹ or bam^Δ86/+ alone.

(Cash and Andrews, 2012)

3 day old females expressing nos^{ALL.T:Hsap\MYC} in a bam^Δ86/+ background show a modest but consistent increase in the average number of spectrosome-containing cells per germarium which is accompanied by a statistically significant loss of differentiated cysts. This effect is more pronounced in 20 day old females.

(Chau et al., 2012)

Expression of how^L.Scer\UAS under the control of the weak driver Scer\GAL4^nos.PG enhances the bam^Δ86/+ phenotype, as shown by the fact that 65% of cysts containing 32 spermatocytes, compared to 27% in bam^Δ86/+ single mutants.

A how^stru-3R-3 heterozygous background results in the generation of 8-cell cysts of spermatocytes and suppresses the extra divisions present in bam^Δ86/+ males.

(Monk et al., 2010)

The fusome phenotype of snf¹⁴⁸ ; bam^Δ86 double mutant ovaries is more similar to that of the snf¹⁴⁸ single mutant phenotype than that of the bam^Δ86 single mutant phenotype.

(Chau et al., 2009)

The delayed differentiation of 16-cell male germline cysts until they are greater than 32-cells/cyst in animals expressing stg^Scer\UAS.cNa under the control of Scer\GAL4^{bam.T:Hsim\VP16} is enhanced from 11% to 27% in a bam^Δ86/+ mutant background.

The premature differentiation of 8-cell male germline cysts observed in animals expressing trbl^{Scer\UAS.P\T.cMa} under the control of Scer\GAL4^{bam.T:Hsim\VP16} is suppressed, from 63% to 10%, in a bam^Δ86/+ mutant background, and the frequency of 16-cell cysts is increased from 37% to 87%.

(Insco et al., 2009)

Most bam^z3-2884/bam^Δ86; eIF-4a/+ ovarioles contain 4-6 normal egg chambers, while the germaria contain fewer single germ cells compared to bam^z3-2884/bam^Δ86 ovarioles.

All of the eIF-4a alleles tested fail to dominantly suppress the tumourous phenotype of bam^Δ86 ovarioles.

The vas^RJ36 or eIF-4E^S058911 allele dominantly suppresses the bam^z3-2884/bam^Δ86 ovariole phenotype.

Scer\GAL4^{nos.UTR.T:Hsim\VP16}-driven expression of eIF-4a^{Scer\UAS.P\T.T:Hsap\MYC} in a bam^Δ86/+ background results in significantly more cystoblasts in the germarium compared to bam^Δ86/+.

(Shen et al., 2009)

bam^Δ86 E2f1⁰⁷¹⁷² double mutant germline stem cell (GSC) clones are much less competitive than bam^Δ86 single mutant GSCs.

bam^Δ86 E2f1^rM729 double mutant germline stem cell (GSC) clones are much less competitive than bam^Δ86 single mutant GSCs.

bam^Δ86 put¹⁰⁴⁶⁰ double mutant germline stem cell (GSC) clones retain a similar ability to bam^Δ86 single mutant GSCs to outcompete wild type GSCs in the same niches.

bam^Δ86 put¹³⁵ double mutant germline stem cell (GSC) clones retain a similar ability to bam^Δ86 single mutant GSCs to outcompete wild type GSCs in the same niches.

(Jin et al., 2008)

Approximately 80% of stwl⁹⁵ bam^Δ86 double mutant female germaria contain germ cells interconnected by branched fusomes, indicating that cystoblasts have formed and initiated cyst differentiation. On average, 30% of germ cells in these ovaries are in clusters penetrated by branched fusomes.

(Maines et al., 2007)

pum⁰²⁰⁰³ bam^Δ86/pum⁹ bam^Δ86 double mutant ovaries have a complex phenotype that is distinct from that of either single mutant. A mixture of apparently undifferentiated cells and overtly polyploid cells is seen. In many cases, the polyploid chromosomes are thick and expanded like nurse cell chromosomes. Occasionally these pseudo-nurse cells are organised within an epithelial layer of follicle cells, like a cyst, although these cysts never contain a full complement of 16 cystocytes. These cysts contain ring canals.

(Chen and McKearin, 2005)

piwi¹ ; bam^Δ86 double mutant ovarioles have mildly tumorous germaria filled with 50-300 undifferentiated germ cells with no apparent egg chamber development. fs(1)Yb⁷² ; bam^Δ86 double mutant ovaries display the same phenotype as seen in bam^Δ86 single mutants. Most germaria are devoid of germ cells in pum¹ bam^Δ86/pum⁹ bam^Δ86 double mutant females.

(Szakmary et al., 2005)

In pelo¹;bam^Δ86 double mutant germaria, germ cells are able to differentiate as cystocytes can form. This is evidenced by branched fusomes, although the morphology of these fusomes is abnormal. However, some germ cells remain undifferentiated, as abnormally enlarged spectrosomes persist. Germ cell differentiation is not rescued when bam^Δ86 homozygous mutants are pelo¹/+; these germaria also show the rapid loss of GSCs observed in pelo¹ germaria.

(Xi et al., 2005)

Clones of female germ cells doubly homozygous for bam^Δ86 and Mad¹² are identical in morphology to bam^Δ86 single mutant germ cells, and do not form germline cysts.

(Casanueva and Ferguson, 2004)

The 'stem cell tumor' phenotype seen in the ovaries of bam^Δ86 homozygous females is suppressed by zpg^unspecified/zpg^unspecified.

(Gilboa et al., 2003)

bam^Δ86 ovarian tumor cells transplanted into the dorsal mesoderm of stage 11-12 embryos from osk^unspecified mothers can repopulate the ovaries of the resulting adults, forming tumorous ovaries. The fertility of these flies can be rescued by heat shock if the transplanted cells carry bam^hs.PO.

(Niki and Mahowald, 2003)

bam^Δ86 put¹³⁵ double mutant germ line clones in the testis behave as bam^Δ86 single mutant clones and overproliferate as spermatogonia-like cells, suggesting that bam is epistatic to put.

(Shivdasani and Ingham, 2003)

bam^Δ86 can dominantly suppress the 32 cell cyst phenotype seen in heatshocked CycA^{Δ.T:Ivir\HA1} flies, reducing the proportion seen five-fold.

(Lilly et al., 2000)

bgcn¹/+ ; bam^z3-2884/bam^Δ86 females show germ cell hyperplasia. None of the germ cells contain the polyploid DNA complement that is diagnostic of nurse cell formation. These females are sterile.

(Ohlstein et al., 2000)

mei-P26¹/Y ; bam^Δ86/+ males are completely sterile. Spermatid differentiation fails to progress in these males. mei-P26¹/mei-P26¹ ; bam^Δ86/+ females show a severe decrease in fertility compared to mei-P26¹/mei-P26¹ females, due to an increase in the formation of ovarian tumours and other egg chamber defects. Recombination is also severely decreased in these females. mei-P26¹/+ ; bam^Δ86/+ females have normal fertility.

(Page et al., 2000)

bgcn^QS2; bam^Δ86 double mutant germ cells also are interconnected and undergo S phase in synchrony. The double mutant germ cells, just like germ cells in either single mutant behave as amplifying primary or secondary spermatogonial cells, and not as stem cells, indicating that bam and bgcn are not partially redundant with each other.

(Gonczy et al., 1997)

Xenogenetic Interactions

Statement

Reference

Crei\I-CreI^hs.PR germaria in which DNA damage has been evoked by heat-shock accumulate cystoblast-like single cells and this accumulation is strongly enhanced by bam^Δ86 heterozygosity.

(Ma et al., 2016)

Expression of Dsim\bam^{bam.T:Avic\GFP-YFP.Venus} restores fertility of the otherwise sterile bam^BG/bam^Δ86 males (even under sperm exhaustion conditions).

Expression of Dsim\bam^{bam.T:Avic\GFP-YFP.Venus} partially restores fertility of the otherwise sterile bam^Δ59/bam^Δ86 females. The ovaries of the rescue females show multiple morphological defects that accumulate with age (loss of germline stem cells, improper number of cyst divisions, mitotic synchrony defects). However, when two copies of the Dsim\bam^{bam.T:Avic\GFP-YFP.Venus} transgene are used, the fertility of the rescue females is reduced (compared to one copy rescue) and they become virtually sterile by day 15. They also show accelerated loss of the germline stem cells from the ovaries and often lack them or any germline cells altogether. These effects are alleviated even by a single copy of endogenous wild-type bam. The partial fertility of bam^Δ59/bam^Δ86;Dsim\bam^{bam.T:Avic\GFP-YFP.Venus} xenogenetic rescue flies is also improved by Wolbachia pipientis infection.

The ovarian defects observed in bam^Δ59/bam^Δ86;Dsim\bam^{bam.T:Avic\GFP-YFP.Venus} xenogenetic rescue females cannot be exacerbated by combination with a single copy of bgcn¹.

(Flores et al., 2015)

Complementation and Rescue Data

Fails to complement

bam^z3-2884

(Ohlstein et al., 2000)

Rescued by

bam¹/bam^Δ86 is rescued by bam^hs.PO

(Kim et al., 2017)

bam^Δ86/bam^Δ59 is rescued by bam^4.1.Venus

(Flores et al., 2015)

bam^Δ86 is rescued by bam^-198+133.GFP

(Chen and McKearin, 2003)

bam^Δ86 is rescued by bam^UASp.GFP/Scer\GAL4^VP16.bam

(Chen and McKearin, 2003)

bam^Δ86 is rescued by bam^-898+133.GFP

(Chen and McKearin, 2003)

bam^Δ86 is rescued by bam^-498+133.GFP

(Chen and McKearin, 2003)

Partially rescued by

bam^Δ86/bam^BG is partially rescued by bam^4.1.Venus

(Flores et al., 2015)

bam^Δ86 is partially rescued by bam^hs.PO

(Ohlstein and McKearin, 1997)

Not rescued by

bam^Δ86 is not rescued by bam^-96+133.GFP

(Chen and McKearin, 2003)

Comments

Expression of bam^{4.1.T:Avic\GFP-YFP.Venus} restores fertility of the otherwise sterile bam^Δ59/bam^Δ86 females and bam^BG/bam^Δ86 males, although under sperm exhaustion conditions the rescue is not complete. Ovaries of the rescue females have wild-type morphology.

The fertility of bam^Δ59/bam^Δ86;bam^{4.1.T:Avic\GFP-YFP.Venus} rescue flies is neither increased nor diminished by Wolbachia pipientis infection.

(Flores et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

5427

ry⁵⁰⁶ e¹ bam^Δ86/TM3, ry^RK Sb¹ Ser¹

Kyoto

108227

ry⁵⁰⁶ e¹ bam^Δ86/TM3, ry^RK Sb¹ Ser¹

Notes on Origin

Discoverer

D. McKearin.

(Bopp et al., 1993)

Comments

Distribution of Sxl product in ovaries is abnormal, the transtition from cytoplasmic to nuclear distribution does not occur. Most if not all of the small germ cells that populate the tumorous cysts show high levels of Sxl protein. Small amount of male specific Sxl splicing can be detected in the ovaries but the level of Sxl protein is not affected.

(Bopp et al., 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

Bam^Δ86

(Easwaran et al., 2022, Osman and Pek, 2018)

bam⁸⁶

(Liu et al., 2023, Ji et al., 2017, Liu et al., 2017, Chang et al., 2013, Hernández et al., 2013, Chen et al., 2009, Maines et al., 2007, Yang et al., 2007, Kalmykova et al., 2005, Xi et al., 2005, Gilboa et al., 2003, Page and Hawley, 2000, Gonczy et al., 1997, McKearin and Ohlstein, 1995)

bam^D86

(Niki, 2006, Casanueva and Ferguson, 2004)

bam^Delta86

(Cook et al., 2009.11.25)

bam^delta86

(Laktionov et al., 2018)

bam^Δ86

(Huang et al., 2024, Baker et al., 2023, Gui et al., 2023, Monteiro et al., 2023, Wenzel and Aquadro, 2023, Zhang et al., 2023, Berry et al., 2022, Ilyin et al., 2022, Kitzman et al., 2022, Lim et al., 2022, Mathieu et al., 2022, Chen et al., 2021, Yu et al., 2021, DeLuca et al., 2020, Ji et al., 2019, Primus et al., 2019, Flora et al., 2018, Maksimov et al., 2018, Kim et al., 2017, Ma et al., 2017, Shan et al., 2017, Carreira-Rosario et al., 2016, Li et al., 2016, Ma et al., 2016, Sanchez et al., 2016, Dolezal et al., 2015, Flores et al., 2015, Kao et al., 2015, Liu et al., 2015, Luo et al., 2015, Sieber and Spradling, 2015, Sun et al., 2015, Chen et al., 2014, Herzig et al., 2014, Pan et al., 2014, Tseng et al., 2014, Zhang et al., 2014, Barton et al., 2013, Eun et al., 2013, Joly et al., 2013, Mathieu et al., 2013, Cash and Andrews, 2012, Chau et al., 2012, Insco et al., 2012, Eliazer et al., 2011, Kirilly et al., 2011, Pek and Kai, 2011, Zhou et al., 2011, Gan et al., 2010, Monk et al., 2010, Tastan et al., 2010, Chau et al., 2009, Insco et al., 2009, Insco et al., 2009, Iovino et al., 2009, Li et al., 2009, Rhiner et al., 2009, Shen et al., 2009, Jiang et al., 2008, Jin et al., 2008, Neumuller et al., 2008, Maines et al., 2007, Roper, 2007, Mikhaylova et al., 2006, Niki et al., 2006)

bam^Δ[supi]86

(Mikhaylova et al., 2006)

bamD86

(Blatt et al., 2021)

bamΔ86

(Lieber et al., 2019, Liu et al., 2010)

bamΔ⁸⁶

(Wylie et al., 2014)

Δ86

(Chen et al., 2020, Yu et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (108)

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