FlyBase Allele Report: Dmel\abd-A[hs.PS]

General Information

Symbol

Dmel\abd-A^hs.PS

Species

D. melanogaster

Name

heat shock construct of Sampedro

FlyBase ID

FBal0030435

Feature type

allele

Associated gene

Dmel\abd-A

Associated Insertion(s)

Carried in Construct

P{HS-abd-A}

Also Known As

hs-abdA

Key Links

Transgenic product class

cDNA

(Sanchez-Herrero et al., 1994)

wild_type

(Sanchez-Herrero et al., 1994)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Sanchez-Herrero et al., 1994)

wild_type

(Sanchez-Herrero et al., 1994)

Mutagen

in vitro construct

(Moore et al., 1998, Sanchez-Herrero et al., 1994, Gonzalez-Reyes et al., 1992)

Progenitor genotype

Carried in construct

P{HS-abd-A}

Cytology

Description

abd-A cDNA, including 5' leader and 3' untranslated sequences, is under control of an inducible Hsp70 promoter.

(Sanchez-Herrero et al., 1994)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

abd-A

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death

(Cenci and Gould, 2005)

lethal - all die before end of larval stage | recessive

(Sanchez-Herrero et al., 1994)

some die during larval stage | recessive

(Sanchez-Herrero et al., 1994)

Phenotype Manifest In

Detailed Description

Statement

Reference

Induction of abd-A^hs.PS expression at 48 hours leads to the elimination of all abdominal neuroblasts by 72 hours.

(Cenci and Gould, 2005)

Expression of abd-A^hs.PS using a heat shock at 48-52 hours after larval hatching results in a 50% reduction in size in all three abdominal postembryonic neuroblast lineages compared to controls.

(Bello et al., 2003)

Ubiquitous expression of abd-A^hs.PS transforms anterior segments into extra A2 segments.

(Chauvet et al., 2000)

When heat shocked 4 hours after egg laying, a complete or almost complete pattern of ectopic A- and B- glial cells in T1-T3 develops. Heat shocks at 7 hours after egg laying have no effect.

(Prokop and Technau, 1994)

Heat induced expression between 3 and 5 hours of development causes transformation of thoracic segments to A2-A6 identity. Transformation of the first abdominal segment to a more posterior abdominal segment, A2-A6 identity. Appearance of two segments on the head as shown be the denticle belts. Posterior structures are not transformed. Same transformation is seen when coexpressed with Antp^hs.PHTA. Heat induced expression at the end of the third larval period or the beginning of pupation causes transformation of the first abdominal segment into a more posterior segment identity. Coexpression with Ubx^hs.PG shows similar transformation to expression of abd-A^hs.PS alone, thought it is difficult to ascertain the phenotypic result as abd-A^hs.PS intermediate transformations resemble those of Ubx^hs.PG embryos. Coexpression with Dfd^hs.PK suggests phenotypic suppression of Dfd by abd-A is dependent on gene dosage.

(Sanchez-Herrero et al., 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

abd-A^hs.PS has increased cell death phenotype, suppressible by grh³⁷⁰

(Cenci and Gould, 2005)

Phenotype Manifest In

Suppressed by

Statement

Reference

abd-A^hs.PS has larval abdomen & neuroblast phenotype, suppressible by grh³⁷⁰

(Cenci and Gould, 2005)

Additional Comments

Genetic Interactions

Statement

Reference

In contrast to when abd-A^hs.PS expression is induced at 48 hours in a wild-type background, this induction fails to cause apoptosis of abdominal neuroblasts in a grh³⁷⁰ background.

(Cenci and Gould, 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

abd-A^hs.PS

Name Synonyms

heat shock construct of Sampedro

Secondary FlyBase IDs

FBal0039122

References (15)

Report Sections

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