FB2024_03 , released June 25, 2024
Allele: Dmel\Egfrtop-101
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General Information
Symbol
Dmel\Egfrtop-101
Species
D. melanogaster
Name
FlyBase ID
FBal0029753
Feature type
allele
Associated gene
Dmel\Egfr
Associated Insertion(s)
Carried in Construct
Also Known As
top101
Key Links
Genomic Maps

Allele class

amorphic allele - genetic evidence

hypomorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - genetic evidence

(Price et al., 1989)

hypomorphic allele - genetic evidence

(Clifford and Schupbach, 1989)
Mutagen
ethyl methanesulfonate
(Clifford and Schupbach, 1994, Clifford and Schupbach, 1989)
Progenitor genotype
Cytology
Description

Nucleotide substitution: C3666T. Amino acid replacement: P1054S.

(Clifford and Schupbach, 1994)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2R:21,558,108..21,558,108 [+]
Nucleotide change:

C21558108T

Reported nucleotide change:

C3666T

Amino acid change:

P1054S | Egfr-PA; P1103S | Egfr-PB

Reported amino acid change:

P1054S

(Clifford and Schupbach, 1994)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Malpighian tubules in homozygous embryos are shorter than wild type, posterior tubules are more strongly affected than the anterior. Reduction in size of the tubules is due to reduction in cell number, not cell death.

      (Baumann and Skaer, 1993)

      Homozygotes show a weak Egfr phenotype: the cuticle is more intact than in stronger Egfr alleles, but shows reductions in the ventral plate and H piece of the anterior head skeleton. There are variable reductions in the number and size of ventral setae and occasional ventral or dorsal midline defects. Many embryos fail to complete germ band retraction.

      (Clifford and Schupbach, 1992)

      Weak 'flb' phenotype when homozygous and hemizygous. Embryos produced from heteroallelic combination with Egfrt1 have a severe ventralised phenotype, reduction in size of their dorsal appendage. Individuals are fully viable over the pupal lethals Egfrtop-CA and Egfrtop-EC20 and show a reduction in viability over Egfrtop-EB.

      (Clifford and Schupbach, 1989)

      Head skeleton is defective, germ band fails to retract and abdominal denticle belts are reduced in width.

      (Price et al., 1989)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressor of
      Statement
      Reference

      Egfrtop-101 is a suppressor of phenotype of rhohs.sev

      (Freeman, 1994)
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Mutation of Egfr that affects the gene function required for embryogenesis, a class II lesion. The allelic series for class II lesions: Egfrtop-101 < Egfrtop-JE14 = Egfrf4 < Egfrf6.

      (Clifford and Schupbach, 1989)

      Germline clone analysis indicates that there is very little, if any, requirement for Egfr in the germline.

      (Clifford and Schupbach, 1992)

      Weak mutation.

      (Baumann and Skaer, 1993)

      Class IIA allele. Class IIA alleles do not disrupt tissue specific gene functions. Class II alleles fully or partially complement the developmental defects of Egfrt1 and Egfrtop-CA. Substantially complements the postembryonic lethality of Egfrtop-EC20. Several combinations of Egfr alleles involving Egfrtop-101, Egfrf4, Egfrf8 and Egfrf6 demonstrate interallelic complementation.

      (Clifford and Schupbach, 1994)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      References (8)