FB2024_03 , released June 25, 2024
Allele: Dmel\scat1
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General Information
Symbol
Dmel\scat1
Species
D. melanogaster
Name
FlyBase ID
FBal0028547
Feature type
allele
Associated gene
Dmel\scat
Associated Insertion(s)
P{PZ}scat1
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Patel et al., 2020, Fári et al., 2016)
Mutagen
P-element activity
(Castrillon et al., 1993)
Progenitor genotype
Associated Insertion(s)
P{PZ}scat1
Cytology
Description

P{PZ} insertion in the third exon.

(Fári et al., 2016)

P{PZ} insertion in an exon.

(Levis, 2000.10.25)
Allele components
Component
Use(s)
Inserted element
P{PZ}
Encoded product / tool
lacZ
Tagged with
Tag:NLS(P)
Mutations Mapped to the Genome
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  motor neuron disease
      CEA
      (Wilkinson et al., 2021, Patel et al., 2020)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      scat1/scat1 and scat1/Df(2L)ED680 males are completely sterile and their seminal vesicles are devoid of mature sperm, but scat1/scat1 females are fertile. All early steps of spermatogenesis appear to be normal in scat1/scat1 mutants, but in post-meiotic stages, scattered spermatid bundles are observed in post-meiotic cysts and very few needle-shaped spermatid nuclei are observed, although no abnormalities are seen in partially elongated cysts; acrosomes appear to be missing and there are defects in the acroblasts; there is a significant decrease in the number of investment cones, the process of individualization fails to start, the elongated cysts lose their integrity and the cells scatter and die, but this is not accompanied by a defect in chromatin condensation, as compared to controls.

      (Fári et al., 2016)

      Mutants show male sterility, with highly elongated, immobile, blebbed sperm tails, disrupted individualisation complexes and scattered spermatid nuclei. Mild nuclear shaping defects are evident.

      (Fabrizio et al., 1998)

      Elongated spermatid nuclei are dispersed.

      (Castrillon et al., 1993)

      sterile when heterozygous with Df(2L)30A-C

      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      FBal0363142:, scat1 is rescued by scatTag:HA/scatTag:HA

      (Patel et al., 2020)

      scat1 is rescued by scatUASp.RFP(Unk)/Scer\GAL4bam.PU

      (Fári et al., 2016)
      Comments

      Expression of scatScer\UAS.P\T.T:Disc\RFP under the control of Scer\GAL4bam.PU completely rescues the male sterility seen in scat1/scat1 mutants.

      (Fári et al., 2016)
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer

      A. Spradling.

      Comments
      Comments

      Complements: numb03235.

      (BDGP Project Members, 1994-1999)

      Excision of the insertion completely reverts the male sterile phenotype.

      (Fári et al., 2016)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      ms(2)05289
      (Levis, 2000.10.25, Spradling et al., 1999)
      ms(2)0528905289
      scat05289
      scat1
      (O'Brien et al., 2023, Wilkinson et al., 2021, Patel et al., 2020, Fári et al., 2016)
      Name Synonyms
      Secondary FlyBase IDs
        References (10)