FlyBase Allele Report: Dmel\Su(var)205[4]

General Information

Symbol

Dmel\Su(var)205⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0016506

Feature type

allele

Associated gene

Dmel\Su(var)205

Associated Insertion(s)

Carried in Construct

Also Known As

Su(var)2-5⁰⁴, Su(var)205⁰⁴, Su(var)2-5⁴

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Eissenberg and Hartnett, 1993, Eissenberg et al., 1992)

Progenitor genotype

Cytology

Description

Nonsense mutation in the C-terminal domain leading to deletion of the domain required for its localisation to the nucleus.

(Kellum and Alberts, 1995)

Amino acid replacement: K169term.

(Eissenberg and Hartnett, 1993, Eissenberg et al., 1992)

Nucleotide substitution: A1201T.

(Eissenberg and Hartnett, 1993, Eissenberg et al., 1992)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:8,209,951..8,209,951 [-]

Nucleotide change:

A8209951T

Reported nucleotide change:

A1201T

Amino acid change:

K169term | Su(var)205-PA; K169term | Su(var)205-PB

Reported amino acid change:

K169term

(Eissenberg et al., 1992)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle | larval stage (with Su(var)205⁵)

(Cenci et al., 2015)

abnormal neuroanatomy | larval stage (with Su(var)205⁵)

(Kuang et al., 2014)

increased cell death (with Su(var)205⁵)

(Perrini et al., 2004)

lethal (with Su(var)205²)

(Norwood et al., 2004)

lethal | recessive

(Lu et al., 2000, Eissenberg and Hartnett, 1993, Eissenberg et al., 1992)

lethal - all die during embryonic stage | maternal effect | recessive

(Kellum and Alberts, 1995)

non-enhancer of variegation, with P{hsp26-pt-T}39C-5

(Boivin et al., 2003)

non-enhancer of variegation, with P{hsp26-pt-T}39C-27

(Boivin et al., 2003)

non-suppressor of variegation, with P{hsp26-pt-T}39C-5

(Boivin et al., 2003)

non-suppressor of variegation, with P{hsp26-pt-T}39C-27

(Boivin et al., 2003)

non-suppressor of variegation | dominant, with P{hsp26-pt-T}39C-5

(Doheny et al., 2008)

some die during embryonic stage | recessive | maternal effect | recessive

(Kellum and Alberts, 1995)

suppressor of variegation

(Cryderman et al., 2011)

suppressor of variegation | dominant, with In(1)w^m4

(Doheny et al., 2008)

suppressor of variegation | dominant, with In(1)w^m4h

(Eissenberg et al., 1992)

suppressor of variegation | dominant, with P{SUPor-P}KV00108

(Glenn and Geyer, 2019)

suppressor of variegation | dominant, with P{SUPor-P}KV00135

(Glenn and Geyer, 2019)

suppressor of variegation | dominant, with P{SUPor-P}KV00590

(Glenn and Geyer, 2019)

Phenotype Manifest In

chromosome, telomeric region

(Perrini et al., 2004)

chromosome, telomeric region (with Su(var)205⁵)

(Perrini et al., 2004, Cenci et al., 2003)

chromosome | larval stage (with Su(var)205⁵)

(Cenci et al., 2015)

imaginal disc

(Fanti et al., 1998)

imaginal disc (with Su(var)205⁵)

(Perrini et al., 2004)

larval neuroblast (with Su(var)205⁵)

(Cenci et al., 2015)

meiosis & nuclear chromosome | male

(Fanti et al., 1998)

melanotic mass (with Df(2L)TE29Aa-11)

(Minakhina and Steward, 2006)

melanotic mass (with Su(var)205²)

(Minakhina and Steward, 2006)

mitosis & nuclear chromosome (with Su(var)205⁵)

(Cenci et al., 2003)

neuroblast & larval brain

(Fanti et al., 1998)

neuroblast | larval stage (with Su(var)205⁵)

(Kuang et al., 2014)

polytene chromosome (with Su(var)205⁵)

(Deng et al., 2007)

polytene chromosome | male (with Su(var)205⁵)

(Spierer et al., 2005)

Detailed Description

Statement

Reference

Su(var)205⁴ partially suppresses the abdominal body or eye pigmentation variegation induced by P{SUPor-P}KV00135, P{SUPor-P}KV00108 or P{SUPor-P}KV00590.

(Glenn and Geyer, 2019)

In Su(var)205⁴/Su(var)205⁵ homozygous larvae, neuroblasts exhibit telomeric fusions, particularly in autosomes.

(Cenci et al., 2015)

Severe neuroblast loss is seen in the brains of Su(var)205⁴/Su(var)205⁵ mutant larvae. Many of the remaining neuroblasts exhibit necrosis markers, including aggregation of mitochondria and the presence of ubiquitin-conjugated protein aggregates.

(Kuang et al., 2014)

Su(var)205⁴ suppresses the position effect variegation of the w gene that is seen in the P{hsp26-pt-T}Dyrk3^118E-15 insertion.

(Cryderman et al., 2011)

One copy of Su(var)205⁴ strongly suppresses position effect variegation (PEV) at the w locus caused by In(1)w^m4.

One copy of Su(var)205⁴ is unable to suppress the telomeric position effect (TPE) in stocks carrying a variegating P{hsp26-pt-T}39C-5 insertion at the telomere of the left arm of chromosome two.

(Doheny et al., 2008)

Polytene chromosomes of Su(var)205⁴/Su(var)205⁵ larvae partly lose the distinct pattern of band-interband regions, especially in the male X chromosome.

(Deng et al., 2007)

Su(var)205²/Su(var)205⁴ mutant third instar larvae have melanotic nodules.

(Minakhina and Steward, 2006)

The X chromosome is bloated in polytene chromosome squashes from male Su(var)205⁴/Su(var)205⁵ third instar larvae.

(Spierer et al., 2005)

Larval imaginal discs of Su(var)205⁴/Su(var)205⁵ mutants exhibit extensive apoptosis. Telomeres in Su(var)205⁴ heterozygous strains are elongated with respect to the wild-type phenotype. In comparison with wild-type telomeres, the telomeres from Su(var)205⁴ heterozygous strains show an increase in HeT-A sequences and a terminal addition of TART-element sequences. Multiple telomere fusions are seen in metaphase neuroblasts of Su(var)205⁴/Su(var)205⁵ larvae.

(Perrini et al., 2004)

Su(var)205⁴ has no effect on the telomeric position effect (TPE) of P{hsp26-pt-T}39C-5 or P{hsp26-pt-T}39C-27.

(Boivin et al., 2003)

Su(var)205⁴/Su(var)205⁵ larval mitotic chromosomes show telomeric fusions.

(Cenci et al., 2003)

Heterozygotes show no effect on crossing over between kni and p^p.

(Westphal and Reuter, 2002)

Neuroblasts in the brain of Su(var)205¹/Su(var)205⁴ or Su(var)205⁴/Su(var)205⁵ larvae show a high frequency of abnormal metaphase configurations, including telomere-telomere fusions. The imaginal discs of Su(var)205⁴/Su(var)205⁵ larvae are reduced in size. Abnormal metaphase configurations showing telomeric fusions are seen in imaginal disc cells. Abnormal meiotic chromosomes are seen in the male germline.

(Fanti et al., 1998)

Homozygous embryos are found to be mostly devoid of intact nuclei. Any remaining nuclei have defects in chromosome segregation and abnormal morphology (chromatin bridges, chromosome lagging during anaphase and under- or over-condensed nuclei). Mitotic synchrony is lost in a high proportion of embryos and defective nuclei are seen beneath the normal plane of nuclei, they appear to be falling into the interior of the embryo.

(Kellum and Alberts, 1995)

mod(mdg4)^neo129 suppresses the strong dominant suppressor effect.

(Dorn et al., 1993)

homozygous lethal

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Su(var)205⁵/Su(var)205⁴ has abnormal neuroanatomy | larval stage phenotype, suppressible | partially by Scer\GAL4^wor.PA/fzy^UAS.cKa

(Kuang et al., 2014)

Su(var)205²/Su(var)205⁴ has lethal phenotype, suppressible | partially by Hsap\CBX5^hs.PN

(Norwood et al., 2004)

NOT suppressed by

Statement

Reference

Su(var)205⁵/Su(var)205⁴ has lethal phenotype, non-suppressible by JIL-1^z2

(Deng et al., 2007)

NOT Suppressor of

Statement

Reference

Su(var)205[+]/Su(var)205⁴ is a non-suppressor of lethal | recessive phenotype of JIL-1^z2

(Deng et al., 2007)

Other

Statement

Reference

Su(var)205⁴, peo¹/peo[+] has abnormal mitotic cell cycle | larval stage phenotype

(Cenci et al., 2015)

Su(var)205⁴, Sxl^fP7B0 has partially lethal - majority die | dominant | maternal effect phenotype

(Li et al., 2011)

Su(var)205⁴, Sxl^f1 has partially lethal - majority die | dominant | maternal effect phenotype

(Li et al., 2011)

P{P-Sal}Pak3^P-Sal, P{lacW}Dplac, Su(var)205⁴ has non-suppressor of variegation phenotype

(Bushey and Locke, 2004)

Phenotype Manifest In

Suppressed by

Statement

Reference

Su(var)205⁵/Su(var)205⁴ has neuroblast | larval stage phenotype, suppressible | partially by Scer\GAL4^wor.PA/fzy^UAS.cKa

(Kuang et al., 2014)

NOT suppressed by

Statement

Reference

Su(var)205⁵/Su(var)205⁴ has polytene chromosome phenotype, non-suppressible by JIL-1[+]/JIL-1^z2

(Deng et al., 2007)

Enhancer of

Statement

Reference

Su(var)205⁴ is an enhancer of eye phenotype of Hers^UAS.Tag:FLAG, Scer\GAL4^GMR.PU

(Ito et al., 2012)

NOT Suppressor of

Statement

Reference

Su(var)205[+]/Su(var)205⁴ is a non-suppressor of polytene chromosome phenotype of JIL-1^z2

(Deng et al., 2007)

Su(var)205⁵/Su(var)205⁴ is a non-suppressor of polytene chromosome phenotype of JIL-1^z2

(Deng et al., 2007)

Other

Statement

Reference

Su(var)205⁴, peo¹/peo[+] has larval neuroblast phenotype

(Cenci et al., 2015)

Su(var)205⁴, peo¹/peo[+] has FlyBase_internalproperty type:chromosome:chromosome | larval stage phenotype

(Cenci et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Flies carrying one copy of T(2;3)Sb^V frequently display a mosaic thoracic bristle phenotype, wherein bristles are both short and long, a phenotype that is enhanced by one copy of Su(var)205⁴.

(Glenn and Geyer, 2019)

peo¹, Su(var)205⁴ double heterozygosity leads to telomeric fusions in larval neuroblasts.

(Cenci et al., 2015)

Expression of fzy^Scer\UAS.cKa under the control of Scer\GAL4^wor.PA suppresses the neuroblast loss seen in Su(var)205⁴/Su(var)205⁵ mutant larval brains. However, these surviving neuroblasts display phenotypes indicative of mitotic catastrophe including multinucleation and enlargement of cell diameter.

(Kuang et al., 2014)

The Scer\GAL4^GMR.PU, Hers^{Scer\UAS.T:Zzzz\FLAG} rough eye phenotype is significantly enhanced by Su(var)205⁴.

(Ito et al., 2012)

In progeny from Su(var)205⁴ heterozygous females crossed to Sxl^fP7B0/Y males, female viability is dramatically reduced.

In progeny from Su(var)205⁴ heterozygous females crossed to Sxl^f1/Y males, female viability is dramatically reduced.

(Li et al., 2011)

The defects in polytene chromosome morphology seen in Su(var)205⁴/Su(var)205⁵ larvae are not altered by one copy of JIL-1^z2.

The defects in polytene chromosome morphology seen in JIL-1^z2 homozygous larvae are not altered by one copy of Su(var)205⁴ or Su(var)205⁵.

The polytene chromosome morphology of Su(var)205⁴/Su(var)205⁵ ; JIL-1^z2/JIL-1^z2 double mutants resembles that of JIL-1^z2/JIL-1^z2 single mutants.

(Deng et al., 2007)

When P{P-Sal}89D is present, the w gene of the P{lacW}ci^Dplac insertion variegates, resulting (in a w^- background) in an eye that is mostly white with a few patches of red ommatidia. This P-element-dependent silencing of P{lacW}ci^Dplac is not dominantly suppressed by Su(var)205⁴.

(Bushey and Locke, 2004)

Has no effect on the frequency of X-Y chromosome nondisjunction seen in Df(1)X-1-53B males.

(McKee et al., 2000)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\Cbx5^hs.PN using daily heat shock in Su(var)205²/Su(var)205⁴ flies results in rescue of flies to adulthood (56% of the expected number of flies are rescued).

(Norwood et al., 2004)

Complementation and Rescue Data

Fails to complement

Su(var)205¹⁴⁹

(Lu et al., 2000)

Rescued by

Su(var)205²/Su(var)205⁴ is rescued by Su(var)205^hs.lacI(DBD)

(Li et al., 2003)

Su(var)205⁴ is rescued by Su(var)205^hs.PE

(Kellum and Alberts, 1995)

Partially rescued by

Su(var)205⁵/Su(var)205⁴ is partially rescued by Su(var)205^{Tag:BTS,Tag:proteinA}

(Alekseyenko et al., 2014)

Su(var)205⁵/Su(var)205⁴ is partially rescued by Su(var)205^hs.PE

(Fanti et al., 1998)

Comments

Su(var)205^{T:Zzzz\BTS,T:Saur\A} rescues the lethality of Su(var)205⁴/Su(var)205⁵ to 58%.

(Alekseyenko et al., 2014)

The abnormal metaphase configurations seen in the brains of Su(var)205⁴/Su(var)205⁵ larvae are partly rescued by expression of Su(var)205^hs.PE, although a high frequency of chromosome aberrations are seen.

(Fanti et al., 1998)

Lethality is rescued by expression of the heat shock activated Su(var)205 construct. Rescued adults behave lethargically, walk slowly and never fly, and die several days after eclosion.

(Eissenberg and Hartnett, 1993)

Images (0)

Mutant

Wild-type

Stocks (2)

Kyoto

101823

In(1)w^m4h; Su(var)205⁴ / In(2L)t In(2R)Cy, Duox^Cy amos^Roi-1 pr¹ cn¹

101824

In(1)w^m4h ; Su(var)205⁴ / In(2L)t In(2R)Cy, Duox^Cy amos^Roi-1 pr¹ cn¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

2-5⁰⁴

(McKee et al., 2000)

HP1⁰⁴

(Johansson et al., 2007)

HP1a⁰⁴

(Wu and Yan, 2022)

Su(var)2-5⁴

(Doheny et al., 2008, Boivin et al., 2003, Eissenberg and Hartnett, 1993, Eissenberg et al., 1992)

Su(var)205⁰⁴

(Schoelz et al., 2021, Cenci et al., 2015, Xing and Li, 2015, Alekseyenko et al., 2014, Frydrychova et al., 2008, Minakhina and Steward, 2006, Shaffer et al., 2006, Bi et al., 2005, Raffa et al., 2005, Marin et al., 2000, Pak et al., 1997)

Su(var)205³

Su(var)205⁴

(Kuang et al., 2014, Ito et al., 2012, Simmons et al., 2012, Li et al., 2011, Simmons et al., 2010, Haigh and Lloyd, 2007)

Suvar2-5⁰⁴

(Kellum and Alberts, 1995)

su(var)205⁰⁴

(Minakhina et al., 2007)

Name Synonyms

Secondary FlyBase IDs

FBal0016505

References (57)

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