FlyBase Allele Report: Dmel\pb[27]

General Information

Symbol

Dmel\pb²⁷

Species

D. melanogaster

Name

FlyBase ID

FBal0013531

Feature type

allele

Associated gene

Dmel\pb

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - genetic evidence

loss of function allele

Mutagen

X ray

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Cribbs et al., 1992, Pultz et al., 1988)

loss of function allele

(Percival-Smith and Hayden, 1998)

Mutagen

X ray

Progenitor genotype

Cytology

Polytene chromosomes normal. (deletion)

Description

Small deletion removing the second exon of pb, which encodes a significant proportion of the protein.

(Percival-Smith and Hayden, 1998)

5kb deletion.

(Pultz et al., 1988)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible

(Percival-Smith et al., 1997)

visible | recessive

(Percival-Smith and Bondy, 1999)

visible | somatic clone

(Percival-Smith et al., 1997)

Phenotype Manifest In

arista | ectopic | heat sensitive (with pb²⁰), with pb^Q50K.hs

(Percival-Smith et al., 1997)

labellum

(Cribbs et al., 1992)

maxillary palp

(Percival-Smith and Bondy, 1999, Percival-Smith et al., 1997)

maxillary palp (with pb²⁰)

(Percival-Smith et al., 2013)

proboscis

(Percival-Smith and Bondy, 1999)

proboscis & sex comb | ectopic

(Percival-Smith and Hayden, 1998)

proboscis | somatic clone

(Percival-Smith et al., 1997)

pulvillus | ectopic | somatic clone

(Percival-Smith et al., 1997)

sex comb | ectopic

(Percival-Smith and Hayden, 1998)

tarsal segment | ectopic | somatic clone

(Percival-Smith et al., 1997)

unguis | ectopic | somatic clone

(Percival-Smith et al., 1997)

Detailed Description

Statement

Reference

The maxillary palps of pb²⁷/pb²⁰ mutant flies are reduced to vestigial stumps.

(Percival-Smith et al., 2013)

pb²⁷/pb²⁰ flies have reduced maxillary palps and a proboscis to tarsus transformation.

(Percival-Smith and Bondy, 1999)

pb²⁷ clones of ectodermal proboscis cells adopt aristal identity. Clones of pb²⁷ cells in the proboscis form sex combs.

(Percival-Smith and Hayden, 1998)

50% of mitotic clones in the proboscis adopt aristal identity, 15% a mixed aristal and tarsal identity and 35% tarsal claws and pulvilli. Infrequently clones in the tarsus exhibit a transformation. pb²⁷/pb²⁰ hemizygotes exhibit reduced maxillary palps. Heat induced expression of pb^Q50K.hs in pb²⁷/pb²⁰ mutants at 36.5^oC for 15-30 minutes between 67 and 72 hours AEL transforms the tarsal mouthparts to arista. Transformation is not associated with pseudotrachea formation. Clones of pb^αTub84B.PP in pb²⁷/pb²⁰ mutants exhibit rescue of the pb null phenotype: the antenna is transformed to a maxillary palp, the reduced maxillary palp is rescued and the tarsal mouthparts are rescued to a proboscis. Clones of pb^{Q50K.αTub84B} in pb²⁷/pb²⁰ mutants display antenna that are not transformed to maxillary palps, the reduced maxillary palps are not rescued and the tarsal mouthparts are transformed towards antenna. pb²⁷ Scr² mosaic clones in the proboscis transform to an aristae and to a third antennal segment with second antennal segment bristle. Clones in the tarsus never exhibit transformation. Clones of pb²⁷ Scr² cells in the ectoderm (proboscis precursor cells) of pb²⁷/pb²⁰ mutants adopt tarsal identity and not aristal identity as in a pb⁺ organism. pb²⁷ clones in the proximal proboscis in pb²⁷/pb²⁰ background have a pb phenotype.

(Percival-Smith et al., 1997)

The labial palp is transformed to prothoracic leg.

(Cribbs et al., 1992)

leg

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Scr², hth^5E04, pb²⁷, put^p1 has visible | somatic clone phenotype

(Joulia et al., 2006)

Scr², hth^5E04, pb²⁷, put^p1, ss^a has visible | somatic clone phenotype

(Joulia et al., 2006)

Scr², hth^5E04, pb²⁷ has visible | somatic clone phenotype

(Joulia et al., 2006)

Mmus\Hoxa2^αTub84B.PP, pb²⁷/pb²⁰ has visible phenotype

(Percival-Smith and Bondy, 1999)

Mmus\Hoxa2^αTub84B.PP, pb²⁷ has visible | recessive phenotype

(Percival-Smith and Bondy, 1999)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

pb²⁷/pb²⁰ has maxillary palp phenotype, non-suppressible by Scr^UAS.cPb/Scer\GAL4^pb.PJ

(Percival-Smith et al., 2013)

Other

Statement

Reference

Scer\GAL4^pb.PJ, Scr^{ΔLASCY.DYTQL.UAS}, pb²⁷/pb²⁰ has proboscis | ectopic phenotype

(Percival-Smith et al., 2013)

Scer\GAL4^pb.PJ, Scr^{ΔDYTQL.CTD.UAS}, pb²⁷/pb²⁰ has proboscis | ectopic phenotype

(Percival-Smith et al., 2013)

Scr², hth^5E04, pb²⁷ has bract | ectopic | somatic clone phenotype

(Joulia et al., 2006)

Scr², hth^5E04, pb²⁷ has arista | ectopic | somatic clone phenotype

(Joulia et al., 2006)

Scr², hth^5E04, pb²⁷, put^p1 has labellum | somatic clone phenotype

(Joulia et al., 2006)

Scr², hth^5E04, pb²⁷, put^p1 has arista | ectopic | somatic clone phenotype

(Joulia et al., 2006)

Scr², hth^5E04, pb²⁷, put^p1, ss^a has leg | ectopic | somatic clone phenotype

(Joulia et al., 2006)

Scr², hth^5E04, pb²⁷, put^p1, ss^a has labellum | somatic clone phenotype

(Joulia et al., 2006)

Scr², hth^5E04, pb²⁷ has leg | ectopic | somatic clone phenotype

(Joulia et al., 2006)

Scr², hth^5E04, pb²⁷ has antenna | ectopic | somatic clone phenotype

(Joulia et al., 2006)

Scr², hth^5E04, pb²⁷ has unguis | ectopic | somatic clone phenotype

(Joulia et al., 2006)

Scr², hth^5E04, pb²⁷ has labellum | somatic clone phenotype

(Joulia et al., 2006)

Mmus\Hoxa2^αTub84B.PP, pb²⁷/pb²⁰ has proboscis phenotype

(Percival-Smith and Bondy, 1999)

Mmus\Hoxa2^αTub84B.PP, pb²⁷ has proboscis phenotype

(Percival-Smith and Bondy, 1999)

ct^L188, pb²⁷ has pseudotrachea phenotype

(Johnston et al., 1998)

ct^L188, pb²⁷ has labellum phenotype

(Johnston et al., 1998)

exd¹, pb²⁷ has proboscis phenotype

(Percival-Smith and Hayden, 1998)

Scr², exd¹, pb²⁷ has arista phenotype

(Percival-Smith and Hayden, 1998)

Scr², exd¹, pb²⁷ has antenna phenotype

(Percival-Smith and Hayden, 1998)

Scr², exd¹, pb²⁷ has proboscis phenotype

(Percival-Smith and Hayden, 1998)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Scr^Scer\UAS.cPb under the control of Scer\GAL4^pb.PJ does not suppress the vestigial maxillary palp phenotype seen in pb²⁷/pb²⁰ mutant flies.

Expression of Scr^Scer\UAS.cPb under the control of Scer\GAL4^pb.PJ in a pb²⁷/pb²⁰ mutant background results in a normal antenna.

Expression of Scr^{ΔDYTQL.CTD.Scer\UAS} under the control of Scer\GAL4^pb.PJ in a pb²⁷/pb²⁰ mutant background results in an antenna to proboscis transformation: deformed antennae with patches of tricombless, convoluted cuticle are seen. Tricombless cuticle is also seen in the pb²⁷/pb²⁰ mutant vestigial maxillary palp. No evidence of a labial cap and chemosensory bristles is observed in theses patches of tricombless cuticle.

Expression of Scr^{ΔLASCY.DYTQL.Scer\UAS} under the control of Scer\GAL4^pb.PJ in a pb²⁷/pb²⁰ mutant background results in an antenna to proboscis transformation: deformed antennae with patches of tricombless, convoluted cuticle are seen. Tricombless cuticle is also seen in the pb²⁷/pb²⁰ mutant vestigial maxillary palp. No evidence of a labial cap and chemosensory bristles is observed in theses patches of tricombless cuticle.

(Percival-Smith et al., 2013)

pb²⁷ Scr² hth^5E04 clones in the labium result in transformation of the labium to a compact leg appendage identified by claws and bracted bristles in 81% of cases, while in the remaining 19% of cases the transformed structure contains a mix of leg and antennal tissue.

pb²⁷ Scr² hth^5E04 put^p1 clones in the labium show transformation to antennal aristae in 76% of cases and mixed transformations are not seen.

pb²⁷ Scr² hth^5E04 put^p1 ss^a clones in the labium show a fully penetrant transformation to stunted legs.

(Joulia et al., 2006)

Clones triple mutant for pb²⁷, Scr² and exd¹ generated 30-60 hours after egg laying show transformations of arista to tarsus, antenna to second leg and rostrum to ectopic eye, with associated leg. pb²⁷, Scr² clones of ectodermal proboscis cells adopt aristal identity. However, both pb²⁷, exd¹ and pb²⁷, Scr², exd¹ clones of ectodermal proboscis cells adopt tarsal (second leg) identity. Clones of pb²⁷, exd¹ cells in the proboscis are transformed to tarsal identity and in addition they form sex combs. When the clones are of the genotype pb²⁷, Scr², exd¹, no sex combs form.

(Percival-Smith and Hayden, 1998)

Xenogenetic Interactions

Statement

Reference

The pb²⁷/pb²⁰ reduced maxillary palp phenotype is not rescued by expression of Mmus\Hoxa2^αTub84B.PP and the proboscis to tarsus transformation is replaced with a partial transformation of proboscis to arista in these flies.

(Percival-Smith and Bondy, 1999)

Complementation and Rescue Data

Rescued by

pb²⁷/pb²⁰ is rescued by pb^αTub84B.PP

(Percival-Smith and Bondy, 1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

2178

pb²⁷/TM3, Sb¹

Notes on Origin

Discoverer

Pultz.

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

map13

(Cribbs et al., 1992)

pb27

(Jordan et al., 2002)

pb²⁷

(Enriquez et al., 2015, Kuert et al., 2014, Percival-Smith et al., 2013, Joulia et al., 2006, Yasunaga et al., 2006)

pb^map13

(Pultz et al., 1988)

Name Synonyms

Secondary FlyBase IDs

References (14)

Report Sections

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