FlyBase Allele Report: Dmel\nod[4]

General Information

Symbol

Dmel\nod⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0013068

Feature type

allele

Associated gene

Dmel\nod

Associated Insertion(s)

Carried in Construct

Also Known As

nod^b27

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

loss of function allele

Mutagen

gamma ray

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Rasooly et al., 1994, Rasooly et al., 1991)

loss of function allele

(Zhang and Hawley, 1990)

Mutagen

gamma ray

(Rasooly et al., 1994, Theurkauf and Hawley, 1992, Rasooly et al., 1991)

Progenitor genotype

Cytology

Description

Deletion resulting in a frameshift that truncates the nod protein after 40 amino acids.

(Afshar et al., 1995)

8bp deletion resulting in a frameshift.

(Rasooly et al., 1994)

Putative point mutation.

(Knowles and Hawley, 1991)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

X:11,580,925..11,581,109 [+]

Comment:

Mutation consists of an 8bp deletion located in this region resulting in a frameshift and a stop codon after 40 amino acids.

(Afshar et al., 1995, Rasooly et al., 1994)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal meiotic cell cycle

(Hatsumi and Endow, 1992)

abnormal meiotic cell cycle | recessive

(Zhang and Hawley, 1990)

Phenotype Manifest In

meiosis & nuclear chromosome & oocyte

(Hatsumi and Endow, 1992)

Detailed Description

Statement

Reference

Late prometaphase I homozygous oocytes show mispositioning of chromosome 4.

(Hatsumi and Endow, 1992)

Spindle organisation in nod^a/nod⁴ oocytes is generally normal, although asymmetrical spindles or normal spindles with spurs associated with one pole are occasionally seen. The X chromosomes rarely exchange in these oocytes. Spindle orientation of nonexchange chromosomes in nod^a/nod⁴ oocytes is highly variable and always abnormal, and the nonexchange chromosomes may be found free in the cytoplasm, or with both homologs present on the same half spindle. The free nonexchange chromosomes are often associated with spindle-like microtubule bundles. Single nonexchange chromosomes that are associated with a half-spindle are always positioned at the pole, not at the wild-type position between the plate and the pole. When more than one nonexchange chromosome is associated with a single half-spindle, one chromosome is always at the pole, but the other is positioned away from the pole, towards the metaphase plate. Homozygous nod⁴ females have similar oocyte defects as nod^a/nod⁴ females, except that the X chromosomes recombine at normal frequencies in homozygous nod⁴ oocytes, and free X chromosomes are seen at a much lower frequency.

(Theurkauf and Hawley, 1992)

High levels of X and fourth chromosome nondisjunction. Hemizygotes exhibit a strong nod phenotype.

(Zhang and Hawley, 1990)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

nod⁴ has abnormal meiotic cell cycle phenotype, enhanceable by sub[+]/sub¹⁷⁹⁴

(Giunta et al., 2002)

nod⁴ has abnormal meiotic cell cycle phenotype, enhanceable by neb[+]/neb^TE8

(Ruden et al., 1997)

nod⁴ has abnormal meiotic cell cycle phenotype, enhanceable by neb[+]/neb^TE12

(Ruden et al., 1997)

nod⁴ has abnormal meiotic cell cycle phenotype, enhanceable by neb[+]/neb^TE20

(Ruden et al., 1997)

Enhancer of

Statement

Reference

nod[+]/nod⁴ is an enhancer of abnormal meiotic cell cycle phenotype of sub¹⁷⁹⁴

(Giunta et al., 2002)

nod[+]/nod⁴ is an enhancer of abnormal meiotic cell cycle phenotype of neb^TE12

(Ruden et al., 1997)

nod[+]/nod⁴ is an enhancer of abnormal meiotic cell cycle phenotype of neb^TE8

(Ruden et al., 1997)

nod[+]/nod⁴ is an enhancer of abnormal meiotic cell cycle phenotype of neb^TE20

(Ruden et al., 1997)

Other

Statement

Reference

In(1)FM7, nod⁴/nod^a has abnormal meiotic cell cycle phenotype

(Cui and Hawley, 2005)

neb^TE2, nod⁴ has female sterile | dominant phenotype

(Ruden et al., 1997)

Phenotype Manifest In

Enhanced by

Statement

Reference

nod⁴ has phenotype, enhanceable by ncd¹

(Knowles and Hawley, 1991)

Additional Comments

Genetic Interactions

Statement

Reference

In(1)FM7 nod⁴/nod^a females show high levels of X and 4th chromosome nondisjunction (53.2% and 78.9% respectively) compared to control In(1)FM7 nod⁺ females. The majority of apparent 4th chromosome nondisjunction events result from loss of the 4th chromosome.

(Cui and Hawley, 2005)

Ova from neb^TE8/+ (or neb^TE12/+ or neb^TE20/+) nod²/+ (or nod³/+ or nod⁴/+) transheterozygous females show a higher rate of 4th chromosome nondisjunction (up to 21%) than females heterozygous for either mutation alone. Transheterozygotes with neb^TE2 are sterile.

(Ruden et al., 1997)

ncd¹ nod⁴ heterozygous females show an increased level of X and fourth chromosome nondisjunction.

(Knowles and Hawley, 1991)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

nod⁴/nod^a is partially rescued by nod^UASp.EGFP/Scer\GAL4^{VP16.nanos.UTR}

(Cui and Hawley, 2005)

nod⁴/nod^a is partially rescued by Scer\GAL4^{VP16.nanos.UTR}/nod^{NDD4.UASp.EGFP}

(Cui and Hawley, 2005)

Not rescued by

nod⁴/nod^a is not rescued by Scer\GAL4^{VP16.nanos.UTR}/nod^{NDD3.UASp.EGFP}

(Cui and Hawley, 2005)

nod⁴/nod^a is not rescued by Scer\GAL4^{VP16.nanos.UTR}/nod^{NDD1-2.UASp.EGFP}

(Cui and Hawley, 2005)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

3430

FM7a, nod⁴/C(1)DX, y¹ f¹/Dp(1;Y)y⁺; sv^spa-pol

Kyoto

107038

FM7a, nod⁴/Dp(1;Y)y⁺/C(1)DX, y¹ f¹; sv^spa-pol

Notes on Origin

Discoverer

Induced on: In(1)FM7, y^31d sc⁸ w^a B v^Of.

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

nod⁴

nod⁶²⁷

(Hatsumi and Endow, 1992)

nod^b27

(Cui and Hawley, 2005, Afshar et al., 1995, Rasooly et al., 1994, Theurkauf and Hawley, 1992, Knowles and Hawley, 1991, Rasooly et al., 1991, Zhang and Hawley, 1990)

Name Synonyms

Secondary FlyBase IDs

FBal0063839

References (12)

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