FlyBase Allele Report: Dmel\mus304[D1]

General Information

Symbol

Dmel\mus304^D1

Species

D. melanogaster

Name

FlyBase ID

FBal0012647

Feature type

allele

Associated gene

Dmel\mus304

Associated Insertion(s)

Carried in Construct

Also Known As

mus304¹

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Boyd et al., 1981)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q539term.

(Brodsky et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:18,111,608..18,111,608 [+]

Nucleotide change:

C18111608T

Amino acid change:

Q539term | mus304-PA

Reported amino acid change:

Q539term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Brodsky et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal DNA repair

(Brodsky et al., 2000)

abnormal meiotic cell cycle | recessive

(Brodsky et al., 2000)

chemical sensitive (with Df(3L)W4)

(Lee et al., 2012)

chemical sensitive | recessive

(Boyd et al., 1981)

radiation sensitive | recessive

(Brodsky et al., 2000)

visible | recessive

(Brodsky et al., 2000)

Phenotype Manifest In

chromosome (with mus304^D3)

(Beaucher et al., 2012)

macrochaeta

(Brodsky et al., 2000)

Detailed Description

Statement

Reference

mus304^D1/Df(3L)W4 eye discs show severe defects in hydroxyurea-induced G2 arrest.

(Lee et al., 2012)

mus304^D1/mus304^D3 mutants have a similar frequency of single-strand annealing repair (SSA) compared to controls in a P{wIw.FRT} hemizygous assay to study DNA double-stranded break repair when assayed at 32^oC or 38^oC.

(Wei and Rong, 2007)

In the absence of irradiation, mus304^D1 eye discs have normal morphology and a wild-type number and distribution of mitotic cells. The number of mitotic cells is unchanged following irradiation, in contrast to wild-type discs where very few cells are in M phase after irradiation. The presence of mitotic cells in these irradiated discs is not a result of either a delayed onset of or a rapid recovery from cell cycle arrest. The defect in the DNA damage checkpoint is also seen in wing discs. mus304^D1/Df(3L)W4 and mus304^D1/Df(3L)x37 animals have macrochaetae defects (probably due genomic instability resulting in the loss of genetic material at haploinsufficient Minute loci). mus304^D1/mus304^D2 flies have wild-type eyes. The frequency of chromosome aberrations following X-ray irradiation is threefold greater in mus304^D1/Df(3L)W4 cells than in wild type. The spontaneous frequency of chromosomal aberrations in mus304^D1/Df(3L)W4 animals is more than 10-fold greater than in wild type. The majority of aberrations are chromatid or isochromatid breaks. Loss of heterozygosity is elevated compared to wild-type in homozygous, mus304^D1/Df(3L)W4 and mus304^D1/Df(3L)x37 animals. mus304^D1/Df(3L)W4 females show a meiotic chromosome segregation defect, which is manifested as a moderate increase in female exceptional progeny (derived from diplo-X ova) and a pronounced increase in male exceptional progeny (derived from nullo-X ova).

(Brodsky et al., 2000)

Flies are not sensitive to X rays.

(Oliveri et al., 1990)

Hypersensitive to methyl methanesulfonate. Sensitive to 0.008% HN2.

(Boyd et al., 1981)

female-fertile

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

mus304^D3/mus304^D1 is an enhancer of abnormal mitotic cell cycle | third instar larval stage phenotype of tefu^unspecified

(Bi et al., 2005)

Other

Statement

Reference

E(mus304)[+]/PolA1^Emus304, mus304^D1/mus304^D2 has visible phenotype

(Brodsky et al., 2000)

PolA1^Emus304, mus304^D1/mus304^D2 has visible phenotype

(Brodsky et al., 2000)

Phenotype Manifest In

Enhancer of

Statement

Reference

mus304^D1/mus304^D1 is an enhancer of chromosome & neuroblast | third instar larval stage 2 phenotype of mre11^unspecified

(Bi et al., 2005)

mus304^D3/mus304^D1 is an enhancer of chromosome & neuroblast | third instar larval stage 2 phenotype of tefu^unspecified

(Bi et al., 2005)

mus304^D1/mus304^D1 is an enhancer of chromosome & neuroblast | third instar larval stage 2 phenotype of tefu^unspecified

(Bi et al., 2005)

NOT Suppressor of

Statement

Reference

mus304^D1 is a non-suppressor of egg chorion phenotype of okr^AA/okr^RU

(Abdu et al., 2002)

mus304^D1 is a non-suppressor of dorsal appendage phenotype of okr^AA/okr^RU

(Abdu et al., 2002)

mus304^D1 is a non-suppressor of egg chorion phenotype of spn-B^BU

(Abdu et al., 2002)

mus304^D1/mus304⁶ is a non-suppressor of egg chorion phenotype of spn-B^BU

(Abdu et al., 2002)

mus304^D1/mus304⁶ is a non-suppressor of dorsal appendage phenotype of spn-B^BU

(Abdu et al., 2002)

mus304^D1 is a non-suppressor of dorsal appendage phenotype of spn-B^BU

(Abdu et al., 2002)

Other

Statement

Reference

E(mus304)[+]/PolA1^Emus304, mus304^D1/mus304^D2 has macrochaeta phenotype

(Brodsky et al., 2000)

E(mus304)[+]/PolA1^Emus304, mus304^D1/mus304^D2 has eye phenotype

(Brodsky et al., 2000)

E(mus304)[+]/PolA1^Emus304, mus304^D1/mus304^D2 has ommatidium phenotype

(Brodsky et al., 2000)

PolA1^Emus304, mus304^D1/mus304^D2 has macrochaeta phenotype

(Brodsky et al., 2000)

PolA1^Emus304, mus304^D1/mus304^D2 has eye phenotype

(Brodsky et al., 2000)

PolA1^Emus304, mus304^D1/mus304^D2 has ommatidium phenotype

(Brodsky et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

The chromosome fusion phenotype seen in third instar larval neuroblasts of tefu^unspecified mutants is enhanced by mus304^D1/mus304^D3 and strongly enhanced by mus304^D1/mus304^D1.

(Bi et al., 2005)

If mus304^D1/mus304^D2 flies are also heterozygous for E(mus304)¹ they have rough eyes and abnormal or missing bristles.

(Brodsky et al., 2000)

mus304^D1/mus304^D3 has no effect on the frequency of X-Y chromosome nondisjunction seen in Df(1)X-1-53B males, but shows a significant enhancement of meiotic drive in these flies compared to controls.

(McKee et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

922

st¹ mus304^D1/TM3, Sb¹ Ser¹

Kyoto

106126

st¹ mus304^D1/TM3, Sb¹ Ser¹

Notes on Origin

Discoverer

Boyd.

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

304^D1

(McKee et al., 2000)

mus304¹

(Abdu et al., 2002, Brodsky et al., 2000)

mus304^D1

(Beaucher et al., 2012, Lee et al., 2012, Wei and Rong, 2007, Oikemus et al., 2006, Bi et al., 2005, Brodsky et al., 2004)

Name Synonyms

Secondary FlyBase IDs

References (14)

Report Sections

Open Close