FB2024_03 , released June 25, 2024
Allele: Dmel\mus304D3
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General Information
Symbol
Dmel\mus304D3
Species
D. melanogaster
Name
FlyBase ID
FBal0012649
Feature type
allele
Associated gene
Dmel\mus304
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Boyd et al., 1981)
Progenitor genotype
Cytology
Description

Amino acid replacement: V695E.

(Brodsky et al., 2000)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3L:18,112,077..18,112,077 [+]
Nucleotide change:

T18112077A

Amino acid change:

V695E | mus304-PA

Reported amino acid change:

V695E

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Brodsky et al., 2000)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      chromosome (with mus304D1)

      (Beaucher et al., 2012)
      Detailed Description
      Statement
      Reference

      mus304D1/mus304D3 mutants have a similar frequency of single-strand annealing repair (SSA) compared to controls in a P{wIw.FRT} hemizygous assay to study DNA double-stranded break repair when assayed at 32oC or 38oC.

      mus304D3 mutants have a similar frequency of single-strand annealing repair (SSA) compared to controls in a P{wIw.FRT} hemizygous assay to study DNA double-stranded break repair when assayed at 38oC.

      (Wei and Rong, 2007)

      mus304D3 animals show a partial loss of the DNA damage checkpoint in an eye disc assay. Loss of heterozygosity is elevated sixfold compared to wild-type in mus304D3/Df(3L)W4 animals. mus304D3/Df(3L)W4 females show a decrease in meiotic recombination on the second chromosome compared to wild type. The decrease is not uniform in the intervals tested, being most severe in distal and medial intervals, and there is actually a slight increase in the interval that spans the centromere. Homozygous and mus304D3/Df(3L)W4 females also show a meiotic chromosome segregation defect, which is manifested as a moderate increase in female exceptional progeny (derived from diplo-X ova) and a pronounced increase in male exceptional progeny (derived from nullo-X ova).

      (Brodsky et al., 2000)

      A partial deficiency in the excision repair of pyrimidine dimers originally observed in this mutant is now attributed to the presence of a secondary phr mutation, phrmus304-D3.

      (Harris and Boyd, 1993)

      Cells derived from homozygous embryos synthesise DNA at a reduced rate after UV irradiation compared to wild-type cells. Homozygous larval brain ganglia have a reduced ability to synthesise DNA both from an undamaged template and after exposure to N-acetoxy-2-acetylaminofluorene compared to wild-type.

      (Boyd and Shaw, 1982)

      Hypersensitive to methyl methanesulfonate. Not sensitive to 0.008% HN2.

      (Boyd et al., 1981)

      female-fertile sensitive only to MMS

      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhancer of
      Statement
      Reference

      mus304D3/mus304D3 is an enhancer of chromosome & neuroblast | third instar larval stage 2 phenotype of tefuunspecified

      (Bi et al., 2005)

      mus304D3/mus304D1 is an enhancer of chromosome & neuroblast | third instar larval stage 2 phenotype of tefuunspecified

      (Bi et al., 2005)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The chromosome fusion phenotype seen in third instar larval neuroblasts of tefuunspecified mutants is weakly enhanced by mus304D3/mus304D3 and more strongly enhanced by mus304D1/mus304D3.

      (Bi et al., 2005)

      mus304D1/mus304D3 has no effect on the frequency of X-Y chromosome nondisjunction seen in Df(1)X-1-53B males, but shows a significant enhancement of meiotic drive in these flies compared to controls.

      (McKee et al., 2000)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer

      Boyd.

      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      304D3
      (McKee et al., 2000)
      mus3043
      (Brodsky et al., 2000)
      mus304D3
      (Beaucher et al., 2012, Wei and Rong, 2007, Bi et al., 2005)
      Name Synonyms
      Secondary FlyBase IDs
        References (10)