FlyBase Allele Report: Dmel\Pen[mbmB]

FB2024_03 , released June 25, 2024

FB2024_03 , released June 25, 2024

Allele: Dmel\Pen^mbmB

General Information

Symbol

Dmel\Pen^mbmB

Species

D. melanogaster

Name

FlyBase ID

FBal0012106

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

Also Known As

mbmB¹

Key Links

Genomic Maps

Allele class

amorphic allele - molecular evidence

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Serway et al., 2020)

loss of function allele

(Serway et al., 2020)

Mutagen

ethyl methanesulfonate

Progenitor genotype

Cytology

Description

Amino acid replacement: W262term.

(Serway et al., 2020)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

2L:10,058,607..10,058,607 [+]

Nucleotide change:

G10058607A

Reported nucleotide change:

G?A

Amino acid change:

W262term | Pen-PA

Reported amino acid change:

W262term

Comment:

Nonsense mutation (TGG to TGA) at Trp codon.

(Serway et al., 2020)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior

(Heisenberg et al., 1985)

abnormal learning | adult stage | recessive

(Serway et al., 2020)

abnormal locomotor behavior | female limited

(Serway et al., 2009)

abnormal long-term memory | adult stage | recessive

(Serway et al., 2020)

abnormal neuroanatomy | adult stage | recessive

(Serway et al., 2020)

decreased cell number | adult stage

(Serway et al., 2020)

decreased cell number | third instar larval stage

(Serway et al., 2020)

decreased size | adult stage (with Df(2L)BSC50)

(Serway et al., 2020)

decreased size | adult stage (with Pen^c05212)

(Serway et al., 2020)

decreased size | adult stage (with Pen^D14)

(Serway et al., 2020)

decreased size | adult stage | recessive

(Serway et al., 2020)

(de Belle and Heisenberg, 1996)

female sterile (with Df(2L)BSC50)

(Serway et al., 2020)

female sterile (with Pen^c05212)

(Serway et al., 2020)

female sterile (with Pen^D14)

(Serway et al., 2020)

partially lethal - majority die | female limited

(Serway et al., 2009)

Phenotype Manifest In

adult central complex

(de Belle and Heisenberg, 1996)

adult central complex | male

(Serway et al., 2020)

adult Kenyon cell | decreased number

(Serway et al., 2020)

adult mushroom body

(de Belle and Heisenberg, 1996)

adult mushroom body (with Df(2L)BSC50)

(Serway et al., 2020)

adult mushroom body (with Pen^c05212)

(Serway et al., 2020)

adult mushroom body (with Pen^D14)

(Serway et al., 2020)

antennal lobe | female

(Serway et al., 2020)

calyx of adult mushroom body

(Serway et al., 2020, de Belle and Heisenberg, 1996)

calyx of larval mushroom body

(Serway et al., 2009)

(de Belle and Heisenberg, 1996)

larval Kenyon cell | decreased number | third instar larval stage

(Serway et al., 2020)

lobe system of adult mushroom body

(Serway et al., 2020, de Belle and Heisenberg, 1996)

(de Belle and Heisenberg, 1996)

(Serway et al., 2020)

Detailed Description

Statement

Reference

Pen^mbmB homozygous adults exhibit a reduction in mushroom body (MB) calyx (dendritic) volume over the first week of adult life. They also exhibit reduction in the vertical α/α' and medial β/β' lobes, and abnormal axonal projection termination and often a fusion of the β-lobes across the midline.

Pen^c05212/Pen^mbmB, Pen^D14/Pen^mbmB or Df(2L)BSC50/Pen^mbmB adults exhibit a reduction in mushroom body volume.

Pen^mbmB homozygous adults exhibit lower (single trial) olfactory learning and memory performance indexes; anesthesia resistant protein synthesis independent memory deficits at both time intervals post-massed-training; and no memory at either post-spaced-training time point.

Pen^mbmB homozygous adults exhibit a reduction in antennal lobe volume (only in females), CCX (ellipsoid body + fan-shaped body) volume (only in males) and wing area (in both sexes).

(Serway et al., 2020)

Mushroom body calyces in mbmB¹ mutants are reduced to approximately 20-50% volume of controls. Gross brain morphology appears relatively normal.

mbmB¹ heterozygous flies demonstrate comparable patterns of landmark orientation, indicating similar responses to visual stimulation in Buridan's paradigm as control flies.

Despite developing with a 50% reduction in mushroom body volume, mbmB¹ males exhibit normal activity levels.

mbmB¹ flies exhibit wild-type walking velocity.

(Serway et al., 2009)

Flies have a number of brain defects, the exact phenotype depending on the genetic background. In the original genetic background in which it was induced, mbmB¹ produces the following phenotype; the calyx is 1/4 to 1/2 normal size, the peduncle and lobes are thin, the ellipsoid body is open ventrally and the noduli are often misshapen. When placed in a Canton S background, the mushroom body phenotype is similar to that in the original genetic background, but the central complex defects are less penetrant.

(de Belle and Heisenberg, 1996)

Calyces, pedunculi and lobes are small. Weak but positive olfactory learning.

(Heisenberg et al., 1985)

Phenotype similar to that of mbm, but less extreme and mushroom body defect occurs in both males and females; learning is weak in tests using olfactory stimuli.

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Pen^mbmB is partially rescued by Scer\GAL4^ey-OK107/Pen^UASp.cGa

(Serway et al., 2020)

Pen^mbmB is partially rescued by Scer\GAL4^Mef2.247/Pen^UASp.cGa

(Serway et al., 2020)

Pen^mbmB is partially rescued by Scer\GAL4^c747/Pen^UASp.cGa

(Serway et al., 2020)

Not rescued by

Pen^mbmB is not rescued by Scer\GAL4^c747/Pen^ΔIBB.UASp

(Serway et al., 2020)

Pen^mbmB is not rescued by Scer\GAL4^c747/Pen^3xSA.UASp

(Serway et al., 2020)

Pen^mbmB is not rescued by Scer\GAL4^c747/Pen^CASB-.UASp

(Serway et al., 2020)

Pen^mbmB is not rescued by Scer\GAL4^c747/Pen^DIM-.UASp

(Serway et al., 2020)

Pen^mbmB is not rescued by Scer\GAL4^c747/Pen^NLSB-.UASp

(Serway et al., 2020)

Pen^mbmB is not rescued by Scer\GAL4^c747/Pen^S37A.UASp

(Serway et al., 2020)

Pen^mbmB is not rescued by Scer\GAL4^c747/Pen^{S56A.UASp.cGa}

(Serway et al., 2020)

Pen^mbmB is not rescued by Scer\GAL4^c747/Pen^S98A.UASp

(Serway et al., 2020)

Pen^mbmB is not rescued by Scer\GAL4^c747/Pen^SNLSB-.UASp

(Serway et al., 2020)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Pen^mbmB

imp-α2^mbmB

(Serway et al., 2020)

mbmB¹

(Serway et al., 2020, Serway et al., 2009)

mbmB^N806

(Heisenberg et al., 1985)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections