FlyBase Allele Report: Dmel\peb[hnt-1]

General Information

Symbol

Dmel\peb^hnt-1

Species

D. melanogaster

Name

FlyBase ID

FBal0005509

Feature type

allele

Associated gene

Dmel\peb

Associated Insertion(s)

Carried in Construct

Also Known As

hnt^XE81, hnt^XE

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Wilk et al., 2000)

Mutagen

ethyl methanesulfonate

(Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die during embryonic stage | recessive

(Yip et al., 1997, Goldman-Levi et al., 1996, Drysdale et al., 1993, Tearle and Nusslein-Volhard, 1987)

some die during embryonic stage | recessive

(Yip et al., 1997, Goldman-Levi et al., 1996, Drysdale et al., 1993, Tearle and Nusslein-Volhard, 1987)

visible (with peb¹)

(Pickup et al., 2002)

visible | somatic clone

(Pickup et al., 2002)

Phenotype Manifest In

amnioserosa

(Goldman-Levi et al., 1996)

amnioserosa | somatic clone

(Lamka and Lipshitz, 1999)

egg chamber | oogenesis stage S14 (with peb¹)

(Deady et al., 2017)

embryo | dorsal closure stage

(Lamka and Lipshitz, 1999)

embryo | dorsal closure stage | somatic clone

(Lamka and Lipshitz, 1999)

embryo | extended germ band stage | somatic clone

(Lamka and Lipshitz, 1999)

embryonic/larval tracheal dorsal trunk

(Wilk et al., 2000)

embryonic/larval tracheal system

(Wilk et al., 2000)

embryonic/larval tracheal system | posterior

(Wilk et al., 2000)

eye (with peb¹)

(Pickup et al., 2002)

eye | somatic clone

(Pickup et al., 2002)

eye photoreceptor cell | somatic clone

(Pickup et al., 2002)

five-cell ommatidial precursor cluster | somatic clone

(Pickup et al., 2002)

germ band

(Wilk et al., 2000)

mitotic domain 1 | extended germ band stage

(Lamka and Lipshitz, 1999, Goldman-Levi et al., 1996, Tearle and Nusslein-Volhard, 1987)

ommatidial precursor cluster | somatic clone

(Pickup et al., 2002)

oocyte associated follicular epithelium | oogenesis stage S14 (with peb¹)

(Deady et al., 2017)

taenidial fold

(Wilk et al., 2000)

taenidium

(Wilk et al., 2000)

Detailed Description

Statement

Reference

peb¹/peb^hnt-1 and peb¹/peb^EH704a transheterozygous egg chambers show a significant decrease in follicle rupture (ex vivo, octopamine-treated egg chambers), as compared to peb¹ heterozygous controls.

(Deady et al., 2017)

There is a delay in neuronal determination of photoreceptor cell precursor clusters in homozygous clones in the eye disc compared to the adjacent wild-type clusters. The developing photoreceptor cell preclusters show defects in cell arrangement and occasionally cell number, with defects first seem at the five cell precluster stage. The cluster sometimes does not rotate properly. R3/R3 type symmetrical ommatidia (where the R3 or R4 cell has not taken up the correct position) are found at the borders of homozygous clones in the eye. In these ommatidia, either the R3 or R4 cell, or both, is mutant and the chirality of these ommatidia is sometimes also incorrect. Homozygous clones in the eye result in a scar. The scar contains no photoreceptor cells but pigment cells are still present. The photoreceptor cells have developed, but have fallen out of the retinal epithelium onto the lamina of the optic lobe of the brain and the basal fenestrated membrane of the retina. Mutant cells in larval eye discs show depletion or more diffuse localisation of F-actin, especially in the more posterior part of the mutant clone. Mosaic ommatidia have a lower concentration of F-actin in their photoreceptor cells than normal and it is found more basally than in the wild-type neighbouring cells.

(Pickup et al., 2002)

Mutant embryos do not retract their germband during stage 13. In mutants embryos the early aspects of tracheal cell fate specification and morphogenesis are indistinguishable from wild-type. During stages 14 and 15, mutant embryos exhibit several tracheal defects, foremost of which is the constriction and expansion of the dorsal trunk to form sac-like structures. Similar defects are seen in the smaller tracheal branches which collapse and become misshapen. By stage 16 many sacs have pinched off and tubes no longer connect these sacs. the lumen is no longer continuous and secondary branching does not occur. Tracheal defects are most severe in the posterior region of the embryo. The tracheal epithelium in peb^hnt-1 mutant embryos has ultrastructurally normal zonula adherentes that are present apicolaterally as in wild-type. Regions of the trachea are either collapsed or expanded compared with wild-type and in places the lumen is severely reduced in size. There are no obvious defects in either the polarity or the general cytological organisation of the surviving tracheal cells in peb^hnt-1 mutants either prior to or during loss of normal epithelial architecture. In peb^hnt-1 mutant trachae the epicuticle is secreted but there are no taenidial folds in either the collapsed or the expanded regions.

(Wilk et al., 2000)

Genetic mosaic embryos show phenotypes that reveal a correlation between the genotype of the amnioserosa and the success or failure of germ band retraction. 100% of mosaics where germband retraction was successful had peb⁺ tissue in the amnioserosa. 100% of embryos with a fully mutant amnioserosa failed to undergo germ band retraction. The genotype of the anterior or posterior midgut is not correlated with the success or failure of germ band retraction. Analysis of distribution of mutant cells in mosaic amnioserosa suggests that peb function is important in those amnioserosa cells that abut the epidermis.

(Lamka and Lipshitz, 1999)

79% of embryos have abdominal segment 5.0 +/- 0.5 at the posterior tip, indicating a failure of germband retraction.

(Yip et al., 1997)

Homozygous embryos exhibit weak germ band retraction defects. Kr staining reveals abnormalities in the amnioserosa.

(Goldman-Levi et al., 1996)

Germband retraction and dorsal closure fail to occur in homozygous embryos.

(Strecker et al., 1995)

Nondefective in gonad assembly.

(Warrior, 1994)

Within constraints of the embryo being folded due to the hnt mutation, the muscle pattern is normal and movement is vigorous and well coordinated.

(Drysdale et al., 1993)

The germ band does not retract in peb^hnt-1 embryos. Embryos are U-shaped with the head facing posteriorly.

(Tearle and Nusslein-Volhard, 1987)

embryonic lethal no germ band retraction; embryo U-shaped with head facing posteriorly. Germ line clones obtained; no maternal effect in homozygous germ-line clones (Perrimon, Engstrom, and Mahowald, 1989).

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

peb^hnt-1 has phenotype, enhanceable by srp^neo45

(Goldman-Levi et al., 1996)

peb^hnt-1 has phenotype, enhanceable by ush²

(Goldman-Levi et al., 1996)

Suppressed by

Statement

Reference

peb^hnt-1 has mitotic domain 1 | extended germ band stage phenotype, suppressible by InR^hs.PF

(Lamka and Lipshitz, 1999)

peb^hnt-1 has embryo | dorsal closure stage phenotype, suppressible by InR^hs.PF

(Lamka and Lipshitz, 1999)

NOT suppressed by

Statement

Reference

peb^hnt-1 has amnioserosa phenotype, non-suppressible by InR^hs.PF

(Lamka and Lipshitz, 1999)

Other

Statement

Reference

Df(3L)H99, peb^hnt-1 has embryonic/larval tracheal system phenotype

(Wilk et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

The tracheal system in peb^hnt-1/Df(3L)H99 mutant embryos, loses its integrity despite the absence of programmed cell death.

(Wilk et al., 2000)

Blocking amnioserosal cell death by making the mutant embryos also mutant for Df(3L)H99 does not alter the effect of mutant clones. InR^hs.PF partially suppresses the germ band retraction defect of peb^hnt-1 embryos. The amnioserosa apoptosis defect is not suppressed.

(Lamka and Lipshitz, 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

peb¹

(Pickup et al., 2002)

peb¹

(Yip et al., 1997)

Rescued by

peb^hnt-1 is rescued by peb^hs.PY

(Yip et al., 1997)

Comments

The failure of germband retraction can be rescued by peb^hs.PY.

(Yip et al., 1997)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

l(1)EH275a is not allelic to peb; l(1)EH275a¹ complements peb^hnt-1, peb^X001, peb^EH704a and peb¹.

(Yip et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

hnt^XE81

(Kim et al., 2020, Ng et al., 2020, Deady et al., 2017, Wilk et al., 2004, Beller et al., 2002, Beller et al., 2002, Pickup et al., 2002, Reed et al., 2001, Wilk et al., 2000, Lamka and Lipshitz, 1999, Yip et al., 1997, Strecker et al., 1995)

hnt^XE

(Lammel and Saumweber, 2000, Sigrist and Lehner, 1997, Goldman-Levi et al., 1996, Warrior, 1994, Drysdale et al., 1993, Tearle and Nusslein-Volhard, 1987)

peb^hnt-1

Name Synonyms

Secondary FlyBase IDs

References (18)

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