FB2024_03 , released June 25, 2024
Allele: Dmel\Egfrf7
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General Information
Symbol
Dmel\Egfrf7
Species
D. melanogaster
Name
FlyBase ID
FBal0003535
Feature type
allele
Associated gene
Dmel\Egfr
Associated Insertion(s)
Carried in Construct
Also Known As
flb2W74, top2W74
Key Links
Genomic Maps

Allele class

heat sensitive loss of function allele

Mutagen
Nature of the Allele
Allele class

heat sensitive loss of function allele

(Clifford and Schupbach, 1994)
Mutagen
ethyl methanesulfonate
(Clifford and Schupbach, 1994, Clifford and Schupbach, 1989, Tearle and Nusslein-Volhard, 1987)
Progenitor genotype
Cytology
Description

Nucleotide substitution: C3763T. Amino acid replacement: T1086I.

(Clifford and Schupbach, 1994)

Single amino acid substitution in the kinase domain.

(Baumann and Skaer, 1993)

Nucleotide substitution: C3253T. Amino acid replacement: T1087I. This threonine residue in the tyrosine kinase domain is conserved in all members of the EGF receptor class.

(Raz et al., 1991)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2R:21,558,205..21,558,205 [+]
Nucleotide change:

C21558205T

Reported nucleotide change:

C3763T

Amino acid change:

T1086I | Egfr-PA; T1135I | Egfr-PB

Reported amino acid change:

T1086I

(Clifford and Schupbach, 1994, Raz et al., 1991)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
X78918
CAA55521
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      adult cuticle

      (Madhavan and Madhavan, 1995)

      adult spiracle

      (Madhavan and Madhavan, 1995)

      anterior dorsal histoblast nest abdominal 1

      (Madhavan and Madhavan, 1995)

      anterior dorsal histoblast nest abdominal 2

      (Madhavan and Madhavan, 1995)

      anterior dorsal histoblast nest abdominal 3

      (Madhavan and Madhavan, 1995)

      anterior dorsal histoblast nest abdominal 4

      (Madhavan and Madhavan, 1995)

      anterior dorsal histoblast nest abdominal 5

      (Madhavan and Madhavan, 1995)

      anterior dorsal histoblast nest abdominal 6

      (Madhavan and Madhavan, 1995)

      anterior dorsal histoblast nest abdominal 7

      (Madhavan and Madhavan, 1995)

      chaeta

      (Madhavan and Madhavan, 1995)

      embryonic/first instar larval cuticle

      (Lim et al., 1997)

      embryonic/larval tracheal dorsal trunk

      (Wappner et al., 1997)

      embryonic/larval visceral tracheal branch

      (Wappner et al., 1997)

      embryonic ventral epidermis

      (Kim and Crews, 1993)

      head

      (Lim et al., 1997)

      larval RP2 motor neuron

      (Skeath, 1998)

      microchaeta & abdominal sternite

      (Madhavan and Madhavan, 1995)

      microchaeta & abdominal tergite

      (Madhavan and Madhavan, 1995)

      mitotic domain 1 | extended germ band stage

      (Lim et al., 1997)

      posterior dorsal histoblast nest abdominal 1

      (Madhavan and Madhavan, 1995)

      posterior dorsal histoblast nest abdominal 2

      (Madhavan and Madhavan, 1995)

      posterior dorsal histoblast nest abdominal 3

      (Madhavan and Madhavan, 1995)

      posterior dorsal histoblast nest abdominal 4

      (Madhavan and Madhavan, 1995)

      posterior dorsal histoblast nest abdominal 5

      (Madhavan and Madhavan, 1995)

      posterior dorsal histoblast nest abdominal 6

      (Madhavan and Madhavan, 1995)

      posterior dorsal histoblast nest abdominal 7

      (Madhavan and Madhavan, 1995)

      presumptive embryonic/larval central nervous system

      (Lim et al., 1997)

      RP2sib neuron

      (Skeath, 1998)

      spiracular histoblast nest abdominal 1

      (Madhavan and Madhavan, 1995)

      spiracular histoblast nest abdominal 2

      (Madhavan and Madhavan, 1995)

      spiracular histoblast nest abdominal 3

      (Madhavan and Madhavan, 1995)

      spiracular histoblast nest abdominal 4

      (Madhavan and Madhavan, 1995)

      spiracular histoblast nest abdominal 5

      (Madhavan and Madhavan, 1995)

      spiracular histoblast nest abdominal 6

      (Madhavan and Madhavan, 1995)

      spiracular histoblast nest abdominal 7

      (Madhavan and Madhavan, 1995)

      sternite

      (Kopp et al., 1999)

      tergite

      (Kopp et al., 1999)

      ventral histoblast nest abdominal 1

      (Madhavan and Madhavan, 1995)

      ventral histoblast nest abdominal 2

      (Madhavan and Madhavan, 1995)

      ventral histoblast nest abdominal 3

      (Madhavan and Madhavan, 1995)

      ventral histoblast nest abdominal 4

      (Madhavan and Madhavan, 1995)

      ventral histoblast nest abdominal 5

      (Madhavan and Madhavan, 1995)

      ventral histoblast nest abdominal 6

      (Madhavan and Madhavan, 1995)

      ventral histoblast nest abdominal 7

      (Madhavan and Madhavan, 1995)
      Detailed Description
      Statement
      Reference

      In the temperature sensitive combination Egfrtop-CA/Egfrf7 the pleura expands at the expense of the sternites and tergites.

      (Kopp et al., 1999)

      All RP2 and RP2sib neurons are missing.

      (Skeath, 1998)

      At 25oC, approximately 90% of embryos fail to undergo head involution. Development of the cuticle and central nervous system and germband retraction are also abnormal. At 28oC, homozygous embryos have a "faint little ball" phenotype with poor cuticle formation.

      (Lim et al., 1997)

      Mutant embryos show absence of dorsal trunk and reduced visceral branches. Dorsal branch and lateral trunks are normal. The dorsal trunk is rescued in a put135 mutant background.

      (Wappner et al., 1997)

      25-49% of Egfrtop-CA/Egfrf7 flies survive to adulthood (FBrf0049928). The anterior dorsal, posterior dorsal, ventral and spiracular histoblast nests contain fewer cells than in wild-type pupae 6 hours after pupariation. The dorsal histoblast nests remain separate 24 hours after pupariation, in contrast to wild-type where they are fused. The histoblast nests in the anterior segments are less abnormal than those in posterior segments. Adults sometimes have holes in the abdominal cuticle and lack spiracles in the abdominal segments. In less severely affected adults chaetae appear abnormal and the distribution of hairs on the tergite, sternite and pleura is irregular and sparse. The thickness of the cuticle is reduced and spiracles are abnormal.

      (Madhavan and Madhavan, 1995)

      Homozygotes show a severe embryonic lethal phenotype at 29oC and a weak embryonic lethal phenotype at 18oC. Enhances the female sterility and adult morphological defects of Egfrt1. Rarely survives as transheterozygote with the semi-viable Egfrtop-CA allele.

      (Clifford and Schupbach, 1994)

      At 29oC Malpighian tubules in homozygous embryos exhibit a very reduced number of cells. Numbers are close to those of wild type when raised at 18oC. Temperature shift experiments reveal Egfr is required for normal tubule development from 3.25 hours AEL until after 8 hours AEL.

      (Baumann and Skaer, 1993)

      Homozygotes and hemizygotes display a weak 'flb' phenotype. Embryos produced from heteroallelic combination with Egfrt1 have a severe ventralised phenotype, reduction in size of their dorsal appendage.

      (Clifford and Schupbach, 1989)

      Weak lethal phenotype: embryos lack some head and telson structures and have some development of the ventral cuticle.

      (Schejter and Shilo, 1989)

      weak allele

      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      Egfrf7 has embryonic/first instar larval cuticle phenotype, suppressible by Dsor1Su1

      (Lim et al., 1997)

      Egfrf7 has head phenotype, suppressible by Dsor1Su1

      (Lim et al., 1997)

      Egfrf7 has head phenotype, suppressible by Dsor1Su4

      (Lim et al., 1997)
      Suppressor of
      Statement
      Reference

      Egfrf7 is a suppressor of phenotype of rhohs.sev

      (Freeman, 1994)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Failure to undergo head involution at 25oC is partly suppressed if both parents are hemi- or homozygous for either Dsor1Su1 or Dsor1Su4. Lack of cuticle formation at 28oC is partly suppressed by maternal expression of Dsor1Su1.

      (Lim et al., 1997)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      Selected as: Embryonic lethal.

      Comments
      Comments

      Weak allele.

      (Schejter and Shilo, 1989)

      Mutation of Egfr that coordinately affects all gene activities, a class I lesion. The allelic series for class I lesions: Egfrt2 = Egfrt1 < Egfrtop-EC20 < Egfrf7 = Egfrf1 = Egfrflb-2E07 < Egfrtop-EE39 = Egfrtop-ED26 = Egfrf5 < Egfrf9 = Egfrf10 = Egfrf2 = Egfrtop-EE42 = Egfrf11 = Egfrf24 = Egfrf3 = Df(2R)Egfr3.

      (Clifford and Schupbach, 1989)

      Less than 10% of wild type number of ventral epidermal cells expressing P{lacZ}BP28 are evident in mutant embryos. oc expression is greatly reduced along the midline.

      (Kim and Crews, 1993)

      Weak mutation.

      (Baumann and Skaer, 1993)

      Class I allele.

      (Clifford and Schupbach, 1994)
      External Crossreferences and Linkouts ( 2 )
      Crossreferences
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      Synonyms and Secondary IDs (9)
      References (17)