FB2024_03 , released June 25, 2024
Allele: Dmel\Egfrf6
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General Information
Symbol
Dmel\Egfrf6
Species
D. melanogaster
Name
FlyBase ID
FBal0003534
Feature type
allele
Associated gene
Dmel\Egfr
Associated Insertion(s)
Carried in Construct
Also Known As
flb2L65, top2L65
Key Links
Genomic Maps

Allele class

amorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - genetic evidence

(Verheyen et al., 1996, Clifford and Schupbach, 1989, Schejter and Shilo, 1989)
Mutagen
ethyl methanesulfonate
(Clifford and Schupbach, 1994, Clifford and Schupbach, 1989, Tearle and Nusslein-Volhard, 1987)
Progenitor genotype
Cytology
Description

Nucleotide substitution: C3856T. Amino acid replacement: S1117L.

(Clifford and Schupbach, 1994)

Nucleotide substitution: C3347T. Amino acid replacement: S1116L. This amino acid residue is in the C-terminal end of the kinase domain.

(Raz et al., 1991)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2R:21,558,298..21,558,298 [+]
Nucleotide change:

C21558298T

Reported nucleotide change:

C3856T

Amino acid change:

S1117L | Egfr-PA; S1166L | Egfr-PB

Reported amino acid change:

S1117L

(Clifford and Schupbach, 1994, Raz et al., 1991)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
X78920
CAA55523
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The number of limb primordial cells is increased in stage 11 embryos, but the final number of leg disc cells is greatly reduced in stage 15 embryos.

      (Kubota et al., 2000)

      Homozygous embryos show a loss of 3 chordotonal organs per abdominal hemisegment (VChA and two in LCh5).

      (Okabe and Okano, 1997)

      Malpighian tubules in homozygous embryos are four tiny outpushings of the posterior hindgut. Reduction in size of the tubules is due to reduction in cell number, not cell death.

      (Baumann and Skaer, 1993)

      All or nearly all of the ventral epidermal cells are absent in mutant embryos.

      (Kim and Crews, 1993)

      Homozygotes have a severe embryonic phenotype; denticles are missing or severely reduced, and there are head and germ band retraction defects. Complements Egfrf8; a milder embryonic phenotype (in both the cuticle and CNS) than either homozygote is seen in combination with this allele. Shows a rough eye phenotype in combination with EgfrE1.

      (Raz et al., 1991)

      Homozygotes and hemizygotes display a severe 'flb' phenotype. Embryos produced from heteroallelic combination with Egfrt1 have a severe ventralised phenotype, reduction in size of their dorsal appendage. Individuals are fully viable over the pupal lethals Egfrtop-CA and Egfrtop-EC20 and show a reduction in viability over Egfrtop-EB.

      (Clifford and Schupbach, 1989)

      severe allele

      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhancer of
      Statement
      Reference

      Egfr[+]/Egfrf6 is an enhancer of eye phenotype of S1

      (Kolodkin et al., 1994)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The disc phenotype of tkv8 Egfrf6 double mutant embryos is a simple addition of each single mutant phenotype, in which wing discs are lost completely and leg discs are severely reduced.

      (Kubota et al., 2000)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      Selected as: Embryonic lethal.

      Comments
      Comments

      Mutation of Egfr that affects the gene function required for embryogenesis, a class II lesion. The allelic series for class II lesions: Egfrtop-101 < Egfrtop-JE14 = Egfrf4 < Egfrf6.

      (Clifford and Schupbach, 1989)

      Less than 10% of wild type number of ventral epidermal cells expressing P{lacZ}BP28 are evident in mutant embryos. oc expression is greatly reduced along the midline.

      (Kim and Crews, 1993)

      Severe Egfr allele.

      (Raz and Shilo, 1993)

      Severe mutation.

      (Baumann and Skaer, 1993)

      Class IIA allele. Class II alleles fully or partially complement the developmental defects of Egfrt1 and Egfrtop-CA. Substantially complements the postembryonic lethality of Egfrtop-EC20. Several combinations of Egfr alleles involving Egfrtop-101, Egfrf4, Egfrf8 and Egfrf6 demonstrate interallelic complementation.

      (Clifford and Schupbach, 1994)
      External Crossreferences and Linkouts ( 2 )
      Crossreferences
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      Synonyms and Secondary IDs (10)
      References (18)