FlyBase Allele Report: Dmel\Egfr[f4]

General Information

Symbol

Dmel\Egfr^f4

Species

D. melanogaster

Name

FlyBase ID

FBal0003532

Feature type

allele

Associated gene

Dmel\Egfr

Associated Insertion(s)

Carried in Construct

Also Known As

flb^2C82, 2C82, top^2C82

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Clifford and Schupbach, 1989)

Mutagen

ethyl methanesulfonate

(Clifford and Schupbach, 1994, Clifford and Schupbach, 1989, Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Polytene chromosomes normal

(Baker and Rubin, 1989)

Description

Nucleotide substitution: G3825A. Amino acid replacement: G1107S.

(Clifford and Schupbach, 1994)

Nucleotide substitution: G3316A. Amino acid replacement: G1106S. This glycine residue in the C-terminal end of the kinase domain is conserved in most tyrosine kinases.

(Raz et al., 1991)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:21,558,267..21,558,267 [+]

Nucleotide change:

G21558267A

Reported nucleotide change:

G3825A

Amino acid change:

G1107S | Egfr-PA; G1156S | Egfr-PB

Reported amino acid change:

G1107S

(Clifford and Schupbach, 1994, Raz et al., 1991)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal wound healing

(Shen et al., 2013)

lethal - all die during embryonic stage

(Baker and Rubin, 1992, Clifford and Schupbach, 1992)

lethal - all die during embryonic stage | recessive

(Clifford and Schupbach, 1994, Clifford and Schupbach, 1989, Schejter and Shilo, 1989)

some die during embryonic stage

(Baker and Rubin, 1992, Clifford and Schupbach, 1992)

some die during embryonic stage | recessive

(Clifford and Schupbach, 1994, Clifford and Schupbach, 1989, Schejter and Shilo, 1989)

Phenotype Manifest In

Detailed Description

Statement

Reference

Egfr^f4 heterozygous embryos exhibit severe defects in morphogenesis. More than 95% of embryos exhibit a 'faint little ball' phenotype, with the posterior end of the embryo in close proximity to the head, indicating a defect in germband retraction. In less severely 'curled' embryos, holes are visible in the dorsal surface, that typically extend anteriorly into the head.

(Shen et al., 2013)

All RP2 and RP2sib neurons are missing.

(Skeath, 1998)

Mutant embryos show absence of dorsal trunk and reduced visceral branches. Dorsal branch and lateral trunks are normal.

(Wappner et al., 1997)

Malpighian tubules in homozygous embryos are four small outpushings of the posterior hindgut, posterior tubules are more strongly affected than the anterior. Reduction in size of the tubules is due to reduction in cell number, not cell death.

(Baumann and Skaer, 1993)

Suppresses the "Ellipse" phenotype.

(Baker and Rubin, 1992)

Homozygotes show a moderate Egfr phenotype: they undergo partial germ band retraction, secrete more cuticle and produce more ventral denticles than amorphic Egfr alleles.

(Clifford and Schupbach, 1992)

Homozygous embryos have head and germ band retraction defects, and denticles are missing or severely reduced. Complements Egfr^f8; a milder embryonic phenotype (in both the cuticle and CNS) than either homozygote is seen in combination with this allele.

(Raz et al., 1991)

Suppresses the dominant "Ellipse" phenotype.

(Baker and Rubin, 1989)

Homozygotes and hemizygotes display an intermediate 'flb' phenotype. Embryos produced from heteroallelic combination with Egfr^t1 have a severe ventralised phenotype, reduction in size of their dorsal appendage. Individuals are fully viable over the pupal lethals Egfr^top-CA and Egfr^top-EC20 and show a reduction in viability over Egfr^top-EB.

(Clifford and Schupbach, 1989)

Intermediate lethal phenotype: some deterioration of anterior structures, ventral denticle bands are thin and shorter.

(Schejter and Shilo, 1989)

intermediate allele

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressor of

Statement

Reference

Egfr^f4 is a suppressor of phenotype of rho^hs.sev

(Freeman, 1994)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Egfr^f4 is rescued by Scer\GAL4^btl.PS/Egfr^II.UAS

(Wappner et al., 1997)

Comments

Scer\GAL4^btl.PS-mediated expression of Egfr^Scer\UAS.cBa rescues the Egfr^f4 tracheal phenotype. Other aspects of the Egfr phenotype (germ band retraction) are not rescued.

(Wappner et al., 1997)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Selected as: Embryonic lethal.

Comments

Mutation of Egfr that affects the gene function required for embryogenesis, a class II lesion. The allelic series for class II lesions: Egfr^top-101 < Egfr^top-JE14 = Egfr^f4 < Egfr^f6.

(Clifford and Schupbach, 1989)

Intermediate Egfr allele.

(Raz and Shilo, 1993)

Intermediate mutation.

(Baumann and Skaer, 1993)

Class IIA allele. Class II alleles fully or partially complement the developmental defects of Egfr^t1 and Egfr^top-CA. Substantially complements the postembryonic lethality of Egfr^top-EC20. Several combinations of Egfr alleles involving Egfr^top-101, Egfr^f4, Egfr^f8 and Egfr^f6 demonstrate interallelic complementation.

(Clifford and Schupbach, 1994)

External Crossreferences and Linkouts ( 2 )

Crossreferences

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

X78920

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

CAA55523

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

2C82

(Shamloula et al., 2002, Zak et al., 1990)

Egfr^2C82

(Shen et al., 2013)

Egfr^f4

flb

flb^2C82

(Therrien et al., 2000, Chen et al., 1998, Skeath, 1998, Wappner et al., 1997, Raz and Shilo, 1993, Baker and Rubin, 1992, Raz et al., 1991, Baker and Rubin, 1989, Schejter and Shilo, 1989)

flb^2c82

(Baumann and Skaer, 1993)

flb^IIC

(Tearle and Nusslein-Volhard, 1987)

top^2C82

(Clifford and Schupbach, 1994, Clifford and Schupbach, 1989)

top^IIC82

(Clifford and Schupbach, 1992)

Name Synonyms

faint little ball

Secondary FlyBase IDs

References (19)

Report Sections

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