T to A transversion in the final drd intron. This is predicted to create a strong ectopic splice acceptor site that results in the inclusion of an additional 10 nucleotides in the spliced transcript (this aberrant splicing has been confirmed by PCR). The mutant transcript is expected to result in the final 76 amino acids of the wild-type drd protein being replaced with a novel sequence of 45 amino acid residues.
T15136550A
The T to A transversion in the final drd intron creates a strong ectopic splice acceptor site that results in the inclusion of an additional 10 nucleotides in the spliced transcript. This results in 45 novel amino acids replacing the final 76aa of the transcript.
female sterile (with drdlwf)
short lived (with drdlwf)
drd[1] adults exhibit crop distension but decreased fecal spots.
In drd1 homozygous females and hemizygous males the midgut and cardia are severely distended and lack a peritrophic membrane.
drd1 homozygotes exhibit an increase in crop volume compared to wild-type.
drd1 homozygous mutants are smaller than wild-type flies.
drd1 homozygotes exhibit a smaller number of vitellogenic egg chambers compared to wild-type.
drdlwf/drd1 transheterozygotes exhibit an increase in crop volume compared to wild-type.
drdlwf/drd1 transheterozygous females are short-lived, sterile, have a smaller body mass, and fewer vitellogenic egg chambers than drd1/+ heterozygotes.
Most adults die within the first week of adulthood. Newly eclosed flies behave normally but with time exhibit sluggish movements or inability to walk or fly. Death typically occurs within a few hours after initial manifestation of these defects. Sections of brains of males that exhibit locomotor defects show lesions in the central brain and optic lobes exhibit degeneration. Brain glia are abnormal.
Hotta and Benzer.
