dncML homozygous and dncM14/dncML heterozygous mutants exhibit an increase in the number of naturally occurring synaptopods at the type II endings. This increase is eliminated in a Dp(1;2)51b background.
Mutant flies show a significant reduction in the number of rest hours per 24 hours compared to controls.
Some dncML flies are arrhythmic with respect to locomotor activity (only 63.16% are rhythmic).
Flies have a low learning score (0.15) in an olfactory classical conditioning paradigm. Homozygous females are sterile. Fertility is restored to approximately 50% if dnchs.PD is expressed using heat shock.
The point of maximal phase delay of the light induced phase response curve (PRC) differs significantly from that of wild type and the circadian period is significantly shorter.
Flies transformed with the P{dnchs.P} or P{rat\dnchs.P} construct display increased learning scores. Reduced learning and memory.
dncML has abnormal learning | recessive phenotype, suppressible by Rat\RD1hs.PD
dncML/dnc[+] is a suppressor of decreased body size | pupal stage phenotype of Nf1E2
dncML/dnc[+] is a suppressor of abnormal learning | dominant | adult stage phenotype of Fmr13
dncML is a suppressor of abnormal size phenotype of Nf1unspecified
dncML is a non-suppressor of abnormal locomotor rhythm phenotype of Nf1P1
dncML is a non-suppressor of abnormal locomotor rhythm phenotype of Nf1P2
dncML is a non-suppressor of abnormal locomotor rhythm phenotype of Nf1unspecified
Nf1unspecified, dncML has partially lethal phenotype
The low learning score phenotype is completely rescued by Rat\RD1hs.PD expressed using heat shock.
dncML/dncM14 is rescued by dncUAS.cCa/Scer\GAL4Tdc2.PC
dncML is rescued by dncUAS.cCa/Scer\GAL4Tdc2.PC
Expression of dncScer\UAS.cCa exclusively in octopaminergic neurons under the control of Scer\GAL4Tdc2.PC rescues the increase in synaptopods at neuromuscular junction type II endings seen in dncML homozygous and dncM14/dncML heterozygous mutants.
A partial rescue is seen after induction of dnchs.P. A significant increase in the initial learning score is seen. Heat shock pulses four days after hatching partly rescues female fertility.
Enzyme activity is 3% of wild-type.