FlyBase Allele Report: Dmel\comt[1]

General Information

Symbol

Dmel\comt¹

Species

D. melanogaster

Name

FlyBase ID

FBal0001755

Feature type

allele

Associated gene

Dmel\comt

Associated Insertion(s)

Carried in Construct

Also Known As

comt^ST53, comatose^ST53, com^ST53

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Pallanck et al., 1995)

Progenitor genotype

Cytology

Description

Amino acid replacement: S483L.

(Littleton et al., 2001)

Single missense mutation due to at GC to AT transition.

(Pallanck et al., 1995)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:13,260,925..13,260,925 [+]

Nucleotide change:

C13260925T

Amino acid change:

S483L | comt-PA; S483L | comt-PB

Reported amino acid change:

S483L

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Littleton et al., 2001, Pallanck et al., 1995)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Parkinson's disease

CEA

(Babcock et al., 2015)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior

(Chan et al., 2002)

abnormal locomotor behavior | adult stage | progressive | heat sensitive

(Babcock et al., 2015)

abnormal neuroanatomy | adult stage | heat sensitive

(Babcock et al., 2015)

abnormal neurophysiology | heat sensitive

(Littleton et al., 2001, Kawasaki et al., 1998)

abnormal starvation stress response | adult stage

(Babcock et al., 2015)

abnormal starvation stress response | larval stage

(Babcock et al., 2015)

abnormal visual behavior | heat sensitive

(Littleton et al., 1998)

bang sensitive

(Chan et al., 2002)

paralytic | heat sensitive

(Littleton et al., 2001, Littleton et al., 1998, Pallanck et al., 1995)

paralytic | heat sensitive (with comt^clp)

(Sanyal and Krishnan, 2001)

paralytic | recessive | heat sensitive

(Dellinger et al., 2000)

short lived | heat sensitive

(Babcock et al., 2015)

Phenotype Manifest In

Detailed Description

Statement

Reference

comt¹/comt¹ mutants exhibit severely reduced lifespans when reared at 29[o]C, a milder reduced lifespan phenotype when reared at 22[o]C; display normal climbing ability when reared at 22[o]C, but progressive loss of climbing ability after shifting to 29[o]C; loss of dopaminergic neurons in the PPL1 cluster after 48h at 29[o]C; decreased survival during prolonged starvation during adulthood at 22[o]C; normal initiation but defective maintenance of starvation-induced autophagy in the larval fat body, with fewer and larger lysosomes and loss of autophagic flux after 12 hours of starvation; loss of autophagic activity in a subset of PPL1 dopaminergic neurons after adults are placed at 29[o]C for 36 hrs, and impaired lysosome trafficking in the brain after adults are placed at 29[o]C for 48 hrs, as compared with wild type flies.

(Babcock et al., 2015)

Duration of copulation in mutant males is significantly shorter than in wild-type males. Mutant flies show a severe locomotor impairment. Mutant flies take longer to recover from mechanical shock than normal.

(Chan et al., 2002)

Electroretinograms taken from the retina at 38^oC reveal losses of on/off transients in mutants.

(Littleton et al., 2001)

50% of homozygotes become paralysed in 1.53 +/- 0.05 minutes at 36^oC. comt¹/comt^Su1 transheterozygotes show a suppressed paralytic phenotype (the time for 50% paralysis is increased to 3.01 +/- 0.13 minutes at 36^oC) compared to that of comt¹ homozygotes.

(Dellinger et al., 2000)

The action potentials of the dorsal longitudinal flight muscle (DLM) are indistinguishable from wild-type at the permissive temperature. At the restrictive temperature an activity-dependent reduction in the DLM action potential and postsynaptic potential is seen. The number of docked vesicles per active zone is higher than wild-type in the neuromuscular synapses of the coxal muscles (at the restrictive temperature).

(Kawasaki et al., 1998)

In ERG assay, mutants lose the on/off transients at 38^oC, though over a slower time course than for Syx1A^3-69. Recovery of transients at 20^oC is slower than for Syx1A^3-69. ERG phenotypes correlate with paralytic phenotypes: time course of paralysis and recovery for Syx1A mutants is more rapid than for comt mutants.

(Littleton et al., 1998)

Paralysis normally occurs within 1-2 minutes of exposure to 38^oC. comt¹ flies bearing P{hsp70-NSF} exposed to heat shock, followed by one day recovery, are highly resistant to exposure to 38^oC.

(Pallanck et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

comt¹ has short lived | heat sensitive phenotype, enhanceable by alphaSnap[+]/αSnap^l65

(Babcock et al., 2015)

comt¹ has paralytic | recessive | heat sensitive phenotype, enhanceable by ovr¹

(Dellinger et al., 2000)

comt¹ has paralytic | recessive | heat sensitive phenotype, enhanceable by cac^TS2

(Dellinger et al., 2000)

NOT suppressed by

Statement

Reference

comt¹ has short lived phenotype, non-suppressible by sei²/sei²

(Babcock et al., 2015)

comt¹ has abnormal locomotor behavior | adult stage | progressive | heat sensitive phenotype, non-suppressible by sei²/sei²

(Babcock et al., 2015)

comt¹ has abnormal neuroanatomy | adult stage | heat sensitive phenotype, non-suppressible by sei²/sei²

(Babcock et al., 2015)

Enhancer of

Statement

Reference

comt¹ is an enhancer of paralytic | recessive | heat sensitive phenotype of cac^TS2

(Dellinger et al., 2000)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

comt¹ has dopaminergic PPL1 neuron | heat sensitive phenotype, non-suppressible by sei²/sei²

(Babcock et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

sei²/sei² does not significantly change the severity of the shortened lifespan, locomotor defects, or loss of dopaminergic neuron phenotypes of comt¹/comt¹ mutants at 29[o]C.

αSnap^l65/+ significantly enhances the shortened lifespan phenotype of comt¹/comt¹ mutants.

(Babcock et al., 2015)

ovr¹ accelerates the paralysis observed in comt¹ animals at 36^oC (the time for 50% paralysis is reduced). At 36^oC, cac^TS2 comt¹ double mutants show faster paralysis than comt¹ single mutants at this temperature. At 38^oC, cac^TS2 comt¹ double mutants show faster rapid paralysis than in cac^TS2 single mutants at this temperature.

(Dellinger et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

comt¹ is rescued by comt^hs.PP

(Kawasaki et al., 1998)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

92841

comt¹

Notes on Origin

Discoverer

Siddiqi and Benzer.

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

ST53

(Tolar and Pallanck, 1998)

com^ST53

(Littleton et al., 2001, Ganetzky, 1984)

comatose^ST53

(Kawasaki et al., 2002, Lilly et al., 1994)

comt¹

comt^ST53

(Babcock et al., 2015, Chan et al., 2002, Dellinger et al., 2000, Kawasaki et al., 1998, Littleton et al., 1998)

comt^st53

(Sanyal and Krishnan, 2001, Pallanck et al., 1995)

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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