Amino acid replacement: Q791term.
C19170516T
Q791term | brat-PA; Q791term | brat-PB; Q791term | brat-PC; Q791term | brat-PE; Q791term | brat-PF
Q791term
Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
When brain fragments from 10 day old brat18/brat14 mutant larvae are transplanted into adult hosts and incubated for 10 days the cells proliferate extensively and fill the host abdomen. brat18/brat14 tumours are able to pass through the muscle layer and form micrometastases. Cells are also able to pass through the basement membranes that surround the muscle layer of epithelial sheath, indicating that the tumour cells are able to completely pass through all three layers of the epithelial sheath. Each micrometastasis develops in the haemolymph filled space between the epithelial sheath and the basement membrane surrounding the follicular epithelium.
When transplanted into wild-type, brat18/brat14 tumor cells form micrometastases in 15% of ovarioles after 10 days of proliferation. brat18/brat14 brain fragments transplanted into ovoD1 hosts for 7 days then wild-type hosts for 10 days form micrometastases in 20.5% of the ovarioles examined. This is not considered a significant increase. brat18/brat14 tissue serially transplanted in ovoD1 hosts for two 7 day periods then into wild-type hosts for 10 days form micrometastases in 20% of the ovarioles examined (i.e. not a significant change with extended passaging). brat18/brat14 tumor cells that are grown in multiple hosts do not show any change in depth of penetration into the ovariole. All of the micrometastases exhibit a similar size and are found between the epithelial sheath and the follicular epithelium.
The median survival of adult hosts transplanted with brat11/brat14 brain fragments is reduced compared to adult hosts transplanted with wild-type brain fragments. Cells from transplanted brat11/brat14 brain fragments form at least one secondary tumour in the wild-type host in 84% of cases. Imaginal discs from brat11/brat14 larvae form secondary tumours in 53% of hosts.
Hemizygous larval brain tissue transplanted into the abdomens of adult female hosts shows unrestrained and invasive growth.
Few adult escapers.
Wright.