Mutation alters the consensus 3' splice site of the second intron from TAG to TAA. If no splicing occurs, then because of stop codons in the intron, the protein product would terminate after 2001 amino acids and would be missing the C terminal 143 amino acids.
Nucleotide substitution: G7184A.
G19591759A
G7184A
ash111/Df(3L)kto2 is lethal at all temperatures, with lethality occurring during late pupal or pharate stages.
Range of lethality for transheterozygotes extends from the third larval instar for the most severe alleles to the pharate adult stage for the least severe alleles.