FlyBase Allele Report: Dmel\amn[1]

General Information

Symbol

Dmel\amn¹

Species

D. melanogaster

Name

FlyBase ID

FBal0000500

Feature type

allele

Associated gene

Dmel\amn

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - genetic evidence

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Feany and Quinn, 1995)

loss of function allele

(Keene et al., 2006)

Mutagen

ethyl methanesulfonate

(Moore et al., 1998)

Progenitor genotype

Cytology

Description

The open reading frame of the mutant is identical to wild type. The amn mRNA is strongly reduced in the mutant compared to wild type.

(Moore et al., 1998)

FlyBase curator comment: FBrf0081985 suggests that the amn¹ mutation is due to a single base pair mutation which results in a frameshift. However, FBrf0102808 shows that the open reading frame in the amn¹ mutant is identical to wild type, and that the level of mRNA produced by the mutant is reduced compared to wild-type levels.

(Feany and Quinn, 1995)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of alcohol use disorder

CEA

(Larnerd et al., 2023, Moore et al., 1998)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

axon | third instar larval stage

(Guan et al., 2011)

neuromuscular junction | third instar larval stage

(Guan et al., 2011)

Detailed Description

Statement

Reference

As in wild type, amn¹ mutant males exhibit inhibition of the female contact-induced male-male aggression seen in wild type males that have previously been exposed to females.

(Yuan et al., 2014)

Compared with wild-type, amn¹ show a decrease in axonal branch number without affecting synaptic varicosity number or innervation length.

(Guan et al., 2011)

amn¹ mutant larvae show a significant delay in response to noxious heat compared to control flies. These flies also exhibit a delay in their jump response to noxious heat of 9 seconds.

(Aldrich et al., 2010)

amn¹ mutants make choices according to their recent experience and completely ignore the past experience in a 60-60V 1hr delay choice, similar to wild-type. No significant difference is observed between the 1hr Anesthesia-resistant memory (ARM) of a single conditioning event and that followed by a second conditioning event in amn¹ mutants.

The choice PI in the 60-30 V 1hr delay protocol is not significantly different from the 30 V immediate memory PI in amn¹ mutants, compared to wild-type where it is significantly lower.

(Yin et al., 2009)

amn¹ and amn^X8/amn¹ flies are able to learn to associate an odor with a sugar reward (although they have a small but significant initial performance defect), but they forget this association within 60 minutes of training.

(Keene et al., 2006)

Muscles of third instar amn¹ larvae show a reduction in the L-type Ca²⁺ current compared to control larvae.

(Bhattacharya et al., 2004)

Mutant animals, unlike wild-type, do not show significant age-related memory impairment, 1 hour memory in aged flies was not significantly different from young mutant flies.

(Tamura et al., 2003)

In amn¹ homozygotes no effect is seen on the amplitude of the synchronous oscillation of intracellular calcium concentration seen in wild type Kenyon cells.

(Rosay et al., 2001)

Shows defects in memory retention both immediately (initial learning) or 180 minutes (3 hour memory) after training.

(DeZazzo et al., 1999)

Hemizygous males and homozygous females show increased sensitivity to ethanol in an inebriometer assay.

(Moore et al., 1998)

After presentation of electric shock with a first odour or with fresh air, amn¹ flies show a strongly reduced avoidance of a second, different odour compared to wild-type flies.

(Preat, 1998)

amn¹ flies kept in constant darkness have a smaller lamina volume than amn¹ flies kept in constant light, as is also seen for wild-type flies.

(Barth et al., 1997)

In experiments involving 'operant' conditioning, with heat as the aversive unconditioned stimulus, amn¹ exhibits a small decrement in learning per se and subsequently has no detectable memory.

(Mariath, 1985)

It appears that short-term memory is defective in the mutant (in shock-odor tests), with long-term memory being normal.

(Tully and Quinn, 1985)

Groups of amn¹ flies exhibit apparently abnormal acquisition of learning in tests using visual cues.

(Folkers, 1982)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

amn¹ is a suppressor of visible phenotype of H^UAS.cMa, Scer\GAL4^GMR.PF

(Müller et al., 2005)

amn¹ is a suppressor of female sterile phenotype of dnc^M11

(Feany and Quinn, 1995)

Phenotype Manifest In

Suppressor of

Statement

Reference

amn¹ is a suppressor of eye phenotype of H^UAS.cMa, Scer\GAL4^GMR.PF

(Müller et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

amn^28A

(DeZazzo et al., 1999)

amn^X8

(DeZazzo et al., 1999)

amn^chpd

(Moore et al., 1998)

Rescued by

amn^X8/amn¹ is rescued by amn^UAS.cWa/Scer\GAL4^c316

(Keene et al., 2006)

amn¹ is rescued by amn^UAS.cWa/Scer\GAL4^c316

(Keene et al., 2006)

amn¹ is rescued by Scer\GAL4^c316/amn^UAS.cDa

(Tamura et al., 2003)

Comments

amn¹, amn^X8 and amn^28A all fail to complement each other for memory retention either immediately (initial learning) or 180 minutes (3 hour memory) after training.

(DeZazzo et al., 1999)

Images (0)

Mutant

Wild-type

Stocks (2)

5954

108499

Notes on Origin

Discoverer

Comments

Memory decay of rad¹ flies is faster than in amn¹ flies. Cold-induced anaesthesia treatment has little effect on memory.

(Folkers et al., 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

amn¹

(Larnerd et al., 2023, Guan et al., 2011, Aldrich et al., 2010, Yin et al., 2009, van Swinderen, 2007, Keene et al., 2006, Keene et al., 2004, Moore et al., 1998, Feany and Quinn, 1995)

amn^PS801

(Quinn, 2001.4.27)

Name Synonyms

Secondary FlyBase IDs

References (32)

Report Sections

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