FB2024_03 , released June 25, 2024
Allele: Dmel\Adhn1
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General Information
Symbol
Dmel\Adhn1
Species
D. melanogaster
Name
negative
FlyBase ID
FBal0000332
Feature type
allele
Associated gene
Dmel\Adh
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

loss of function allele

amorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - genetic evidence

(Bijlsma and Bijlsma-Meeles, 1991, Hollocher and Place, 1987)

loss of function allele

(Oudman and Kamping, 1991)
Mutagen
ethyl methanesulfonate
Progenitor genotype
AdhS
(Chenevert et al., 1995, Hollocher and Place, 1987)
Cytology
Description

Amino acid replacement: G93E. Nucleotide substitution: G1120A.

(Chenevert et al., 1995)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2L:14,616,681..14,616,681 [+]
Nucleotide change:

G14616681A

Reported nucleotide change:

G1120A

Amino acid change:

G93E | Adh-PC; G93E | Adh-PE; G93E | Adh-PF; G93E | Adh-PH; G93E | Adh-PI

Reported amino acid change:

G92E

Comment:

Induced on an AdhS chromosome.

(Chenevert et al., 1995)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Flies of the transheterozygous mutant genotypes Adhn1/Adhfn6 and Adhn1/Adhfn23 both show the same defective phenotypic response to ethanol. These flies experience a hyperactive phase that is of a much lower magnitude than in wild type. The proportion of akinetic flies is substantially higher amongst the Adh mutants than in wild-type flies and the mutants accumulate higher amounts of ethanol. However, the mutants have a normal startle response to ethanol.

      (Wolf et al., 2002)

      Sensitive to ethanol.

      (Bijlsma and Bijlsma-Meeles, 1991)

      Adhn1 larvae have the same resistance to anoxia as wild-type larvae.

      (Oudman and Kamping, 1991)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (6)
      Notes on Origin
      Discoverer

      E.H. Grell.

      Comments
      Comments

      Enzyme activity: 20% of wild type progenitor. CRM positive. Forms active hybrid enzyme. Polypeptide slightly larger than wild type on SDS gels and electrophoretic mobility altered on non-denaturing gels.

      Ethanol concentration of 3.5% and lower does not cause any additional mortality of Adh- eggs compared to wild type. If 2 hour eggs are subjected to 3.5% ethanol their mortality is higher than for 9 and 18 hour eggs. It is postulated that the membrane is not fully developed at this stage so the eggs are more vulnerable. Survival is less than 5% on ethanol concentrations of 4.3% and 4.5%. Eggs brought into contact with this concentration of ethanol show a higher viability than eggs at a later stage due to a phenotypic increase in ethanol tolerance.

      (Bijlsma and Bijlsma-Meeles, 1991)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Adhn1
      (Hollis et al., 2009)
      Name Synonyms
      negative
      Secondary FlyBase IDs
        References (15)