[15F9-15F9];[16E1-16E1];
A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
15F9;16E1
Breakpoint from FlyBase's release 5 sequence location of progenitor insertion.
The presence of P+PBac{XP5.RB3}BSC641 was verified using the PCR methods and primers described in FBrf0175003, with the substitution of the primer 5'-GCTTCTAAACGCTTACGCATAAACGATG-3' for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in the Supplementary Methods.
The cytological breakpoints of Df(1)BSC641 predicted from the Release 5 genomic coordinates of the insertions sites of the progenitor transposable elements P{XP}Fimd03334 and PBac{RB}CG8142e04583 are 15F9;16E1. The presence of a deletion was confirmed cytologically, though the breakpoints were not analyzed in detail.