Df(3L)DocA/+ ; Df(3R)Exel6157/+ and Df(3L)DocA/+ ; Df(1)CHES-1-like¹/+ double heterozygous embryos show defects in the symmetric cell divisions that give rise to the tin-expressing cardial cells that are significantly more severe than the additive effects of each of the two single heterozygotes. Asymmetric cell division defects in "svp" cardiac progenitor cells are not significantly different from the additive effects of each of the two single heterozygotes.

The pacing-induced heart arrest/fibrillation rate in Df(2L)Exel6012/+; Df(3L)DocA/+ flies is dramatically elevated compared to the single heterozygous controls.

Df(3L)29A6/Df(3L)DocA embryos exhibit mild defects in cardioblast specification.

Df(3L)DocA/Df(3L)EP584MR2 animals are fully viable. A small number of viable Df(3L)Scf-R11/Df(3L)DocA adult escapers are seen. Df(3L)DocA/Df(3L)29A6 animals show pupal lethality.

Df(3L)DocA embryos have significantly reduced neuroblast and daughter proliferation in the brain and significantly reduced numbers of neuroblasts, including type II neuroblasts, compared to controls.

The boundary between the maxillary and mandibular segments is unaffected in Df(3L)DocA embryos.

Df(3L)DocA embryos in which germ band retraction has been rescued by Scer\GAL4^c381-driven Doc2^UAS.cRa expression, are missing odd-expressing pericardial cells and odd-positive cells that originate from the thoracic dorsal mesoderm that normally form lymph glands. In contrast, eve-expressing pericardial cells are unaffected.

Homozygous embryonic lethal. Mutant embryos show defects in germ band retraction, resulting in a u-shaped phenotype. The head appears reduced in size from stage 12 onwards, resulting in reduced head structures and a frequent failure of head involution in later stage embryos. The amnioserosa does not show a properly folded morphology during stages 8-10 and the posterior germ band is forced to bend towards the inside of the embryo. Some abnormally small nuclei become detectable within the amnioserosa during this stage. By late stage 12, almost all amnioserosa cells have small nuclei that are difficult to distinguish from dorsal epidermal cells. Stage 11 mutant embryos show significant incorporation of BrdU in amnioserosa nuclei, whereas no incorporation is seen in wild-type embryos. Mitotic spindles are present in the amnioserosa of mutant stage 11 embryos. Late stage 12 mutant embryos show significant apoptosis in the amnioserosa, which is not seen in wild-type embryos.