A 419bp l(2)gd1 promoter fragment drives expression of a full-length l(2)gd1 cDNA (from the LD23056 clone), in which 5 of the 14 consensus Cdk1 phosphorylation sites have been mutated to a phosphomimetic Glu residue (amino acid substitutions S225E, T239E, T242E, S254E and S474E). The coding sequence is tagged at the N-terminal end with EGFP.