A 419bp l(2)gd1 promoter fragment drives expression of a full-length l(2)gd1 cDNA (from the LD23056 clone), in which 5 of the 14 consensus Cdk1 phosphorylation sites have been mutated to a non-phosphorylatable Ala residue (amino acid substitutions S225A, T239A, T242A, S254A and S474A). The coding sequence is tagged at the N-terminal end with EGFP.