A ubiquitous promoter (snRNA:U6:96Ac) drives expression of four sgRNAs, two of which target orion (target sequences are GAAGGGCAACTACACCCAGG and CATGTTTCGTCGGATCACAG) and two of which target drpr (target sequences are CCATGCCGTAGAATCCAGGT and ACGGACAAGGATGCGCCCAG).
A ubiquitous promoter (snRNA:U6:96Ac) drives expression of four sgRNAs, two of which target orion (target sequences are GAAGGGCAACTACACCCAGG and CATGTTTCGTCGGATCACAG) and two of which target drpr (target sequences are CCATGCCGTAGAATCCAGGT and ACGGACAAGGATGCGCCCAG).