FB2024_03 , released June 25, 2024
Recombinant Construct: P{αTub84B(FRT.y+CD2)wts.TWC}
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General Information
Symbol
P{αTub84B(FRT.y+CD2)wts.TWC}
FlyBase ID
FBtp0160762
Feature type
transgenic_transposable_element
Component Allele(s)
wtsαTub84B.FRT.TWC
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Construct components
Component allele wtsαTub84B.FRT.TWC
Transgenic product class
wild_type
(Vrabioiu and Struhl, 2015)
Component
Use(s)
Regulatory region(s)
αTub84B
Encoded product / tool
wts
Tagged with
Citrine
mTFP1
Also carries
FRT
Description

An FRT cassette separates the αTub84B promoter from sequence encoding full-length wts that is tagged at the N-terminal end with mTFP1 and at the C-terminal end with Citrine. mTFP1 and Citrine can act as a fluorescence resonance energy transfer (FRET) pair (donor and acceptor respectively), and this protein can act as a permuted wts FRET sensor: changes in the quenching ratio (acceptor channel emissions/donor channel emissions) can be used to monitor conformational changes in the wts protein in vivo.

(Vrabioiu and Struhl, 2015)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
P{αTub84B(FRT.y+CD2)wts.TWC}
(FlyBase, 1992-)
Secondary FlyBase IDs
    References (2)