A αTub84B promoter drives expression of full-length wts that is tagged at the N-terminal end with Citrine and at the C-terminal end with mTFP1. mTFP1 and Citrine can act as a fluorescence resonance energy transfer (FRET) pair (donor and acceptor respectively), and changes in the quenching ratio (acceptor channel emissions/donor channel emissions) can be used to monitor conformational changes in the wts protein in vivo. Generated by excision of the FRT cassette in wtsαTub84B.FRT.CWT.